Zobrazeno 1 - 10
of 72
pro vyhledávání: '"V N, Iyer"'
Autor:
Hélène Labbé, Jiro Hattori, Brian Miki, Thérèse Ouellet, Pierre Fobert, V. N. Iyer, Elizabeth Foster, Lisa E. James
Publikováno v:
Plant Molecular Biology. 41:45-55
We have isolated a constitutive promoter sequence, tCUP, from tobacco by T-DNA tagging using a promoterless GUS-nos3′ reporter gene construct. The T-DNA integration event produced a translational fusion with the GUS gene that is expressed widely in
Publikováno v:
Plasmid. 35:58-61
The essential replicon region of plasmid pCU1 has, within 1.2 kb, two origins of replication that can function in the absence of Escherichia coli DNA polymerase I and one that requires this polymerase. To isolate mutants in the replicon pathway that
Autor:
Bin Huang, V. N. Iyer, Chris L. Baszczynski, María-Jesús Ginés, Janice M. DeMoor, Brian Miki, Kim Boutilier
Publikováno v:
Plant Molecular Biology. 26:1711-1723
Brassica napus cv. Topas microspores can be diverted from pollen development toward haploid embryo formation in culture by subjecting them to a heat stress treatment. We show that this switch in developmental pathways is accompanied by the induction
Autor:
Hélène Labbé, Brian Miki, Sylvia G. Gottlob-McHugh, V. N. Iyer, John Cosmopoulos, Thérèse Ouellet, Pierre Fobert, Jiro Hattori, Glen Sunohara
Publikováno v:
The Plant Journal. 6:567-577
T-DNA tagging with a promoterless beta-glucuronidase (GUS) gene generated a transgenic Nicotiana tabacum plant that expressed GUS activity only in developing seed coats. Cloning and deletion analysis of the GUS fusion revealed that the promoter respo
Publikováno v:
Applied and Environmental Microbiology. 56:2866-2869
A region of DNA which determined the production of the insecticidal toxin of Bacillus thuringiensis subsp. israelensis was cloned into a derivative of a broad-host-range group IncQ plasmid vector of gram-negative bacteria. The plasmid which we constr
Autor:
V. N. Iyer, S.-W. Ma
Publikováno v:
Applied and Environmental Microbiology. 56:2206-2212
A collection of 13 field isolates of Rhizobium leguminosarum bv. viciae that have the ability to nodulate the roots of current North American cultivars of peas as well as a “primitive” cultivar, Afghanistan, was examined. These isolates originate
Autor:
V. N. Iyer, B. Rajendra Krishnan
Publikováno v:
Journal of Molecular Biology. 213:777-788
A DNA segment of approximately 2000 base-pairs bounded by restriction enzyme sites for PvuII and containing the minimal replicon of an N group plasmid was characterized. A natural derivative of this miniplasmid was found to have undergone a deletion
Publikováno v:
Plant molecular biology. 41(1)
We have isolated a constitutive promoter sequence, tCUP, from tobacco by T-DNA tagging using a promoterless GUS-nos3' reporter gene construct. The T-DNA integration event produced a translational fusion with the GUS gene that is expressed widely in o
Autor:
Martin Holčiacute, V. N. Iyer
Publikováno v:
Plasmid. 35(3)
PRD1 infects bacteria carrying IncN plasmids by binding to their conjugative pili. Mutations in a plasmid locus kikA close to the pilus region result in PRD1 resistance and reduced conjugation proficiency to Klebsiella but not to Escherichia coli. On
Autor:
S K, Banerjee, V N, Iyer
Publikováno v:
BioTechniques. 18(6)