Zobrazeno 1 - 10
of 22
pro vyhledávání: '"V D, Vacquier"'
Publikováno v:
Glycoconjugate journal. 17(3 -4)
The low density, detergent-insoluble membrane fraction (LD-DIM), where gangliosides are likely to be highly enriched, was prepared from sperm of two sea urchin species, Hemicentrotus pulcherrimus and Strongylocentrotus purpuratus. Immunoblotting show
Publikováno v:
Zygote (Cambridge, England). 8
Publikováno v:
BioEssays : news and reviews in molecular, cellular and developmental biology. 23(1)
Abalone sperm lysin is a non-enzymatic protein that creates a hole for sperm passage in the envelope surrounding the egg. Lysin exhibits species-specificity in making the hole and it evolves rapidly by positive selection. Our studies have focused on
Publikováno v:
Journal of molecular biology. 296(5)
Abalone sperm lysin is a 16 kDa acrosomal protein used by sperm to create a hole in the egg vitelline envelope. Lysins from seven California abalone exhibit species-specificity in binding to their egg receptor, and range in sequence identity from 63
Publikováno v:
Molecular marine biology and biotechnology. 7(1)
A tandemly repeated satellite DNA of 290-291 base pairs (bp) was identified by SalI digestion of genomic DNA of five species of Eastern Pacific (California) abalone (genus, Haliotis). Following cloning and sequencing of one repeat unit from one speci
Autor:
Y H, Lee, V D, Vacquier
Publikováno v:
BioTechniques. 14(2)
Autor:
V D, Vacquier, G W, Moy
Publikováno v:
Methods in enzymology. 201
Publikováno v:
The Journal of biological chemistry. 265(9)
Single crystals of the protein lysin (Mr = 16,070) from the spermatozoa of the red abalone (Haliotis rufescens) have been obtained by the vapor diffusion technique, using as precipitants a 32.5% saturated solution of (NH4)2SO4 (incubation at 18 degre
Autor:
S B, Podell, V D, Vacquier
Publikováno v:
Experimental cell research. 155(2)
The egg jelly-induced acrosome reaction is inhibited by polyclonal antibodies raised against either of two S. purpuratus sperm-membrane proteins, of Mr 80 and 210 kD. Although the two antigens used have dissimilar CNBr peptide maps, antisera produced
Publikováno v:
Advances in experimental medicine and biology. 207
Exposure of Arbacia punctulata spermatozoa to solubilized egg jelly results in the immediate dephosphorylation (within 3 sec) of an abundant 160,000 dalton (160 kDa) sperm membrane protein, and a simultaneous increase in its electrophoretic mobility