Zobrazeno 1 - 10
of 41
pro vyhledávání: '"Uwe Theobald"'
Autor:
Michael Baltes, Uwe Theobald, Matthias Reuss, Manfred Rizzi, Erich Querfurth, Thilo Rohrhirsch
Publikováno v:
Biotechnology and Bioengineering. 49:316-327
In the present study, the glucose transport into the yeast Saccharomyces cerevisiae has been investigated. The approach suggested is based on a rapid sampling technique for studying the dynamic response of the yeast to rapid changes in extracellular
Publikováno v:
Antonie van Leeuwenhoek. 78:307-313
Streptomyces antibioticus Tu 6040 is the producer of simocyclinones, which belong to a novel family of angucyclinone antibiotics some of which show antitumor activities. Growth and antibiotic production is dependent on the medium composition, especia
Publikováno v:
Biotechnology Letters. 22:123-128
A scale-up strategy into 200 l pilot-scale for the production of the antibiotic gallidermin by Staphylococcus gallinarum Tu 3928 was developed. Large-scale fermentations were simulated by consecutive liquid cultures of smaller scale. Afterwards, opti
Publikováno v:
Biotechnology Letters. 21:959-963
The correlation between the consumption of amino acids and the production of the polypeptide antibiotic gallidermin by Staphylococcus gallinarum Tu 3928 was investigated by on-line determination of amino acids and pulse experiments. A prolonged produ
Publikováno v:
Biotechnology and Bioengineering. 55:592-608
A mathematical model of glycolysis in Saccharomyces cerevisiae is presented. The model is based on rate equations for the individual reactions and aims to predict changes in the levels of intra- and extracellular metabolites after a glucose pulse, as
Publikováno v:
Biotechnology and Bioengineering. 55:305-316
The goal of this work was to obtain rapid sampling technique to measure transient metabolites in vivo. First, a pulse of glucose was added to a culture of the yeast Saccharomyces cerevisiae growing aerobically under glucose limitation. Next, samples
Publikováno v:
Journal of Biotechnology. 49:19-27
The glycerol-3-phosphate dehydrogenase has been purified from Saccharomyces cerevisiae 140-fold to electrophoretic homogeneity by a simple procedure involving affinity and ion exchange chromatography. The purified enzyme was most active at pH 6.8 and
Publikováno v:
Biotechnology Letters. 18:461-466
Orthophosphate concentration of baker's yeast Saccharomyces cerevisiae was investigated during dynamic conditions. As an example for those dynamics in cell metabolism the transition from glucose limitation to glucose excess (Crabtree-effect) was choo
Publikováno v:
IFAC Proceedings Volumes. 28:124-129
A mathematical model has been developed to simulate the dynamics of key reactions of the catabolism in growing cells of Saccharomyces cerevisiae. The structured model considers the reactions of glycolysis, tricarbon acid cycle, pentose phosphate shun
Publikováno v:
Biotechnology Techniques. 8:723-728
To investigate dynamic phenomena in yeast cytoplasm a method was developed that guarantees immediate inactivation of cell activity. Differential extraction procedure gains porosity of the outer cell membrane exclusively. Application of the method dur