Zobrazeno 1 - 10
of 60
pro vyhledávání: '"Urban J. Lewis"'
Publikováno v:
Endocrine Journal. 47:S1-S8
This review will summarize the properties of five variant forms of human growth hormone: a disulfide dimer, a glycosylated form, 20 kD-hGH, and two peptides made up of portions of 22 kD-hGH. The two pituitary peptides (hGH(1-43) and hGH(44-191)) have
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d32103fdb997e39ec46bb341b6dc5f23
https://doi.org/10.1507/endocrj.47.supplmarch_s1
https://doi.org/10.1507/endocrj.47.supplmarch_s1
Autor:
Andrew O. Martinez, Urban J. Lewis, N.R. Staten, Gwen G. Krivi, Luis S. Haro, R. N. P. Singh, S.S. Galosy
Publikováno v:
Biochemical and Biophysical Research Communications. 222:421-426
The carboxyl-terminal hGH fragment, hGH 44–191 , displays diabetogenic activity. To understand whether this biological activity is mediated through somatogenic or lactogenic receptors, we investigated the ability of hGH 44–191 to bind both recept
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b88103e036db5709a44927acf4c2fdb8
https://doi.org/10.1006/bbrc.1996.0760
https://doi.org/10.1006/bbrc.1996.0760
Autor:
Urban J. Lewis
Publikováno v:
Trends in Endocrinology & Metabolism. 3:117-121
The evidence is now irrefutable that growth hormone (GH), long thought to be a single substance, is actually a mixture of several different forms. These multiple forms must be a consideration in any physiologic study if an accurate evaluation of the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::79002b9db44eb9857ec03d235925ba93
https://doi.org/10.1016/1043-2760(92)90099-m
https://doi.org/10.1016/1043-2760(92)90099-m
Publikováno v:
Molecular and Cellular Endocrinology. 78:45-54
A modified human growth hormone (hGH) that lacks the first 43 residues of the intact hormone was prepared by recombinant-DNA technology. For preparative purposes an additional alanine was made the amino terminal residue. Sequence analysis and tryptic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b24b2413e2068092629df134d6d2a6c3
https://doi.org/10.1016/0303-7207(91)90184-t
https://doi.org/10.1016/0303-7207(91)90184-t
Autor:
R. N. P. Singh, K.E. Scott, L.V. DePaolo, B.P. Jacobsen, Y. N. Sinha, Urban J. Lewis, L. S. Haro
Publikováno v:
Molecular and cellular endocrinology. 80(1-3)
Four isoforms of glycosylated prolactin (G-pPRL) were isolated from porcine pituitary glands by affinity chromatography and concanavalin A-Sepharose, based upon differences in their affinity for the lectin. Structural analysis indicated differences i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8e59c5c5152cf5da071c6d26b3fbcfd
https://pubmed.ncbi.nlm.nih.gov/1955078
https://pubmed.ncbi.nlm.nih.gov/1955078
Publikováno v:
The Journal of clinical endocrinology and metabolism. 71(2)
The receptor-binding properties of monomeric nonglycosylated human PRL (hPRL), glycosylated hPRL that does not bind to Concanavalin-A-Sepharose (G1-hPRL) and glycosylated hPRL that binds to Concanavalin-A-Sepharose (G2-hPRL) were tested in the lactat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cdd11d2e18e995e1e79a361711555d12
https://pubmed.ncbi.nlm.nih.gov/2380335
https://pubmed.ncbi.nlm.nih.gov/2380335
Publikováno v:
Pancreas. 5(1)
In order to study the possible differential effects of the nonglycosylated and glycosylated forms of prolactin on insulin content and secretion in pancreatic islets, neonatal rat pancreatic islets were exposed for 6 days in vitro to 2 micrograms/ml o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9cd191ba0295911de8d2e11b2489df85
https://pubmed.ncbi.nlm.nih.gov/2403453
https://pubmed.ncbi.nlm.nih.gov/2403453
Publikováno v:
Journal of Protein Chemistry. 2:425-436
A procedure is described for isolation from human pituitary glands of a peptide with the amino acid sequence of the first 43 residues of human growth hormone, hGH (1–43). The peptide was recovered in a yield of 12 mg per 1000 pituitary glands. In a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::84de1cd3e43ff1af8e54e42976fc1417
https://doi.org/10.1007/bf01025416
https://doi.org/10.1007/bf01025416
Publikováno v:
Journal of Protein Chemistry. 2:341-346
A radioimmunoassay for the 15-amino acid fragment comprising residues 32–46 of the 22,000-dalton human growth hormone has been devised. The radioimmunoassay detects from 1 to 150 fmol of the synthetic peptide. The 12-amino acid peptide hGH (35–46
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f7145278d128d1dd6c8f8c92dc531c85
https://doi.org/10.1007/bf01025599
https://doi.org/10.1007/bf01025599
Publikováno v:
Endocrinology. 94:883-891
A proteolytically modified form of human growth hormone, isolated from a pituitary extract, had a growth promoting potency of 6.9 IU/mg in the tibial line assay. The prolactin activity of the material, measured by the crop sac assay, was 14.5 IU/mg.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::94ffac9d561aa47decb4577fb06232ab
https://doi.org/10.1210/endo-94-3-883
https://doi.org/10.1210/endo-94-3-883