Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Unni Spaeren"'
Publikováno v:
Analytical Biochemistry. 96:378-383
Polyoma DNA was cleaved with restriction endonuclease HpaII, the fragments were separated by gel electrophoresis and transferred in good yield to separate nitrocellulose filters by a modification of the procedure of E. M. Southern (1975, J. Mol. Biol
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 395:413-421
Nuclei were isolated from synchronized HeLa cells in the S-phase by a modification of the non-aqueous method described by Kirsch et al. (Science (1970) 168, 1592–1595). The method involved lyophilization of the cells, homogenization in non-aqueous
Publikováno v:
Biochemical Journal. 102:586-592
1. An improved 11000g cell-free system for the incorporation of [(14)C]valine into gramicidin S has been obtained. The cell-free extract used was the supernatant obtained by treating Bacillus brevis with ultrasonics for 1min. followed by centrifugati
Publikováno v:
Scandinavian Journal of Clinical and Laboratory Investigation. 21:65-77
The activities of the following enzymes have been determined in the soluble fraction of liver from rats fed a special basal diet supplemented with the carcinogen 2-acetylaminof luorene for various lengths of time: aspartate carbamoyltransferase, deox
Publikováno v:
Journal of Biological Chemistry. 235:719-725
Publikováno v:
Journal of Biological Chemistry. 237:150-156
Publikováno v:
Biochemical Journal. 130:297-299
Publikováno v:
Journal of molecular biology. 135(3)
Viral nucleoprotein complexes containing radioactive form l DNA or replicative intermediates were extracted from nuclei isolated from polyoma-infected 3T6 fibroblasts, pulse labelled with [3H]thymidine. Such extracts incorporated labelled dGTP into v
Publikováno v:
European journal of cancer. 9(5)
The estrogen binding capacity of 251 human breast cancers has been determined. The cytosol of the tumours was incubated with labelled estradiol-17-β and subjected to sucrose-gradient-density centrifugation. The difference in the radioactivity in the
Publikováno v:
Biochimica et biophysica acta. 272(4)
Ribosomal subunits were isolated from rabbit reticulocyte polysomes after incubation under conditions of protein synthesis followed by treatment with 0.5 M KCl. When both the 40-S and 60-S subunits were incubated in a cell-free system, the heterologo