β-arrestin1 and 2 exhibit distinct phosphorylation-dependent conformations when coupling to the same GPCR in living cells

Autor: Raphael S. Haider, Edda S. F. Matthees, Julia Drube, Mona Reichel, Ulrike Zabel, Asuka Inoue, Andy Chevigné, Cornelius Krasel, Xavier Deupi, Carsten Hoffmann

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-17 (2022)

Here the authors present improved intramolecular sensors for β-arrestin2 and 1, which enable assessment of conformational changes of both isoforms in living cells. These reveal that the same GPCR induces differential conformational rearrangements th

Externí odkaz: https://doaj.org/article/c788b6ac74db47fe8176035c251fe6d0

Development of a Conformational Histamine H3 Receptor Biosensor for the Synchronous Screening of Agonists and Inverse Agonists

Autor: Henry F. Vischer, Steffen Pockes, Martin J. Lohse, Gunnar Schulte, Hannes Schihada, Ulrike Zabel, Rob Leurs, Xiaoyuan Ma

Publikováno v: ACS Sensors
ACS Sensors, 5(6), 1734-1742. American Chemical Society
Schihada, H, Ma, X, Zabel, U, Vischer, H F, Schulte, G, Leurs, R, Pockes, S & Lohse, M J 2020, ' Development of a Conformational Histamine H 3 Receptor Biosensor for the Synchronous Screening of Agonists and Inverse Agonists ', ACS Sensors, vol. 5, no. 6, pp. 1734-1742 . https://doi.org/10.1021/acssensors.0c00397

The histamine H(3) receptor (H(3)R) represents a highly attractive drug target for the treatment of various central nervous system disorders, but the discovery of novel H(3)R targeting compounds relies on the assessment of highly amplified intracellu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6fde1e44567b8801b7f7e0bb4897675
http://europepmc.org/articles/PMC7325232

Stepwise activation of a class C GPCR begins with millisecond dimer rearrangement

Autor: Ralf Schmauder, Martin J. Lohse, Tina Schwabe, Ulrike Zabel, Eugene O. Grushevskyi, Klaus Benndorf, Taulant Kukaj, Andreas Bock

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America

Significance G protein-coupled receptors (GPCRs) form a family of signaling molecules in the membrane of cells that plays a key role in transduction of cellular responses. Little is known about how rapidly GPCRs can be activated. While the “light r

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f26cac7a508c9a0c81ee12809ce038a
https://doi.org/10.1073/pnas.1900261116

Determination of G-protein-coupled receptor oligomerization by molecular brightness analyses in single cells

Autor: Romy Thomas, Ulrike Zabel, Jan Möller, Irene Coin, Paolo Annibale, Marco Scarselli, Annette G. Beck-Sickinger, Chiara De Faveri, Ali Işbilir, Martin J. Lohse, Andreas Bock, Robert Serfling

Publikováno v: Nature protocols. 16(3)

Oligomerization of membrane proteins has received intense research interest because of their importance in cellular signaling and the large pharmacological and clinical potential this offers. Fluorescence imaging methods are emerging as a valid tool

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cee5b66bf400ff15a024dc7c3f76ddef
https://pubmed.ncbi.nlm.nih.gov/33514946

Optical Mapping of cAMP Signaling at the Nanometer Scale

Autor: Charlotte Konrad, Paolo Annibale, Andreas Bock, Ulrike Zabel, Martin J. Lohse, Isabella Maiellaro, Martin Falcke, Sivaraj Sivaramakrishnan, Annette Hannawacker, Selma E. Anton

Publikováno v: Cell

Cells relay a plethora of extracellular signals to specific cellular responses using only a few second messengers, such as cAMP. To explain signaling specificity, cAMP-degrading phosphodiesterases (PDEs) have been suggested to confine cAMP to distinc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d32b728442a5c9c84b148c7384809fd0
https://pubmed.ncbi.nlm.nih.gov/33989549