Side-chain moieties from the N-terminal region of Aβ are Involved in an oligomer-stabilizing network of interactions.

Autor: Kaja Przygońska, Jarosław Poznański, Ulrik H Mistarz, Kasper D Rand, Michał Dadlez

Publikováno v: PLoS ONE, Vol 13, Iss 8, p e0201761 (2018)

Oligomeric forms of the Aβ peptide represent the most probable neurotoxic agent in Alzheimer's disease. The dynamic and heterogeneous character of these oligomers makes their structural characterization by classic methods difficult. Native mass spec

Externí odkaz: https://doaj.org/article/6c483f347d5e480bbe7084892c674c9b

Probing the dissociation of protein complexes by means of gas-phase H/D exchange mass spectrometry

Autor: Kasper D. Rand, Jeffery Mark Brown, Justin L. P. Benesch, Shane A. Chandler, Ulrik H. Mistarz

Publikováno v: Journal of the American Society for Mass Spectrometry. 30(1)

Gas-phase hydrogen/deuterium exchange measured by mass spectrometry (gas-phase HDX-MS) is a fast method to probe the conformation of protein ions. The use of gas-phase HDX-MS to investigate the structure and interactions of protein complexes is howev

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49f986ebf010b8d59d7625411217a250
http://ora.ox.ac.uk/objects/uuid:

Installation, validation, and application examples of two instrumental setups for gas-phase HDX-MS analysis of peptides and proteins

Autor: Kasper D. Rand, Ulrik H. Mistarz

Publikováno v: Methods (San Diego, Calif.). 144

Gas-phase hydrogen/deuterium exchange measured by mass spectrometry in a millisecond timeframe after ESI (gas-phase HDX-MS) is a fast and sensitive, yet unharnessed method to analyze the primary- and higher-order structure, intramolecular and intermo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::578b37661f869c56de6163c8f5d56a04
https://pubmed.ncbi.nlm.nih.gov/29753788

UV Photodissociation Mass Spectrometry Accurately Localize Sites of Backbone Deuteration in Peptides

Autor: Ulrik H, Mistarz, Bruno, Bellina, Pernille F, Jensen, Jeffery M, Brown, Perdita E, Barran, Kasper D, Rand

Publikováno v: Analytical chemistry. 90(2)

Hydrogen/deuterium exchange mass spectrometry (HDX-MS) is now a routinely used technique to inform on protein structure, dynamics, and interactions. Localizing the incorporated deuterium content on a single residue basis increases the spatial resolut

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::cf7cea89b5806bf64a8d6ba0f5f16928
https://pubmed.ncbi.nlm.nih.gov/29266933

Construct design, production, and characterization of Plasmodium falciparum 48/45 R0.6C subunit protein produced in Lactococcus lactis as candidate vaccine

Autor: Robert W. Sauerwein, Michael Theisen, Kasper D. Rand, Will Roeffen, Fen Yang, Ulrik H. Mistarz, Susheel K. Singh, Bishwanath Kumar Chourasia

Publikováno v: Microbial Cell Factories
Microbial Cell Factories, 16, 1, pp. 97
Microbial Cell Factories, 16, 97
Microbial Cell Factories, Vol 16, Iss 1, Pp 1-11 (2017)
Singh, S K, Roeffen, W, Mistarz, U H, Chourasia, B K, Yang, F, Rand, K D, Sauerwein, R W & Theisen, M 2017, ' Construct design, production, and characterization of Plasmodium falciparum 48/45 R0.6C subunit protein produced in Lactococcus lactis as candidate vaccine ', Microbial Cell Factories, vol. 16, no. 1, 97 . https://doi.org/10.1186/s12934-017-0710-0

Background The sexual stages of Plasmodium falciparum are responsible for the spread of the parasite in malaria endemic areas. The cysteine-rich Pfs48/45 protein, exposed on the surface of sexual stages, is one of the most advanced antigens for inclu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d7f6c3e5171334dff8bffbaf321fe11
https://pubmed.ncbi.nlm.nih.gov/28569168