Zobrazeno 1 - 10
of 23
pro vyhledávání: '"Ulla Plappert-Helbig"'
Autor:
Myriam Lemmens, Benoit Fischer, Michael Zogg, Lindsey Rodrigues, Grainne Kerr, Alberto del Rio-Espinola, Fanny Schaeffer, Danilo Maddalo, Valerie Dubost, Alessandro Piaia, Arne Mueller, Ulla Plappert-Helbig, Ulrike Naumann, Jasmin Haegele, Alex Odermatt, Hans-Jörg Martus, Silvana Libertini
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 23, Iss , Pp 241-253 (2021)
Off-target editing is one of the main safety concerns for the use of CRISPR-Cas9 genome editing in gene therapy. These unwanted modifications could lead to malignant transformation, which renders tumorigenicity assessment of gene therapy products ind
Externí odkaz:
https://doaj.org/article/290bd942646a411a99a58b2d82349198
Autor:
Lindsey Rodrigues, Arne Mueller, Jasmin Haegele, Alberto Del Rio-Espinola, Silvana Libertini, Danilo Maddalo, Alex Odermatt, Benoit Fischer, Valerie Dubost, Alessandro Piaia, Myriam Lemmens, Ulla Plappert-Helbig, Grainne Kerr, Fanny Schaeffer, Ulrike Naumann, Hans-Jörg Martus, Michael Zogg
Publikováno v:
Molecular Therapy. Methods & Clinical Development
Molecular Therapy: Methods & Clinical Development, Vol 23, Iss, Pp 241-253 (2021)
Molecular Therapy: Methods & Clinical Development, Vol 23, Iss, Pp 241-253 (2021)
Off-target editing is one of the main safety concerns for the use of CRISPR-Cas9 genome editing in gene therapy. These unwanted modifications could lead to malignant transformation, which renders tumorigenicity assessment of gene therapy products ind
Autor:
Diethilde Theil, Wilfried Frieauff, Silvana Libertini, Hans-Jörg Martus, Ulla Plappert-Helbig
Publikováno v:
Environmental and Molecular Mutagenesis. 60:4-16
The phosphorylation of histone H2AX in Serine 139 (gamma-H2AX) marks regions of DNA double strand breaks and contributes to the recruitment of DNA repair factors to the site of DNA damage. Gamma-H2AX is used widely as DNA damage marker in vitro, but
Autor:
Ulla, Plappert-Helbig, Silvana, Libertini, Wilfried, Frieauff, Diethilde, Theil, Hans-Jörg, Martus
Publikováno v:
Environmental and molecular mutagenesis. 60(1)
The phosphorylation of histone H2AX in Serine 139 (gamma-H2AX) marks regions of DNA double strand breaks and contributes to the recruitment of DNA repair factors to the site of DNA damage. Gamma-H2AX is used widely as DNA damage marker in vitro, but
Autor:
Melanie Guérard, Ulla Plappert-Helbig
Publikováno v:
Environmental and Molecular Mutagenesis. 56:788-793
To compare the extent of potential inter-laboratory variability and the influence of different comet image analysis systems, in vivo comet experiments were conducted using the genotoxicants ethyl methanesulfonate and methyl methanesulfonate. Tissue s
Autor:
Marlies De Boeck, Dan D. Levy, Marie Vasquez, Kamala Pant, Patricia A. Escobar, Andrew Collins, Günter Speit, Peter Kasper, Carol Beevers, Yoshifumi Uno, Hajime Kojima, Stefan Pfuhler, Jin Tanaka, Ulla Plappert-Helbig, Sachiko Kitamoto, Brian Burlinson
Publikováno v:
Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 783:6-12
As a part of the 6th IWGT, an expert working group on the comet assay evaluated critical topics related to the use of the in vivo comet assay in regulatory genotoxicity testing. The areas covered were: identification of the domain of applicability an
Autor:
Ulla Plappert-Helbig, M.R. O'Donovan, Bas-jan van der Leede, Jonathan Howe, Ann T. Doherty, Véronique Thybaud, Melanie Guérard
Publikováno v:
Mutation Research/Genetic Toxicology and Environmental Mutagenesis. :81-88
In genotoxicity testing of pharmaceuticals the rodent alkaline comet assay is being increasingly used as a second in vivo assay in addition to the in vivo micronucleus assay to mitigate in vitro positive results as recommended by the ICH S2(R1) guide
Publikováno v:
Environmental and Molecular Mutagenesis. 55:114-121
The comet assay has increasingly been used for regulatory genotoxicity testing in recent years. While the technique of the comet assay per se on a first glance seems to be relatively “simple”, the experimental execution of the assay such as organ
Publikováno v:
Toxicological Sciences. 137:259-267
Vemurafenib is a first-in-class, small molecule B-Raf kinase inhibitor for the treatment of patients with unresectable or metastatic melanoma carrying the BRAFV600E mutation, commer cially available since 2011. A general phototoxic potential was iden
Autor:
Richard D. Storer, Andreas Rothfuss, Satin Sawant, Ulla Plappert-Helbig, Sheila M. Galloway, Shuichi Hamada, Jonathan Howe, Kazuhiko Saigo, Andreas Czich, Dominique Brault, Andrew R. Kraynak, Michael R. O’Donovan, Bas-jan van der Leede, Melanie Struwe, Makoto Hayashi, Véronique Thybaud, Madoka Nakajima, Marlies De Boeck, Esther Vock, Laura Custer, Catherine C. Priestley, Jing Shi
Publikováno v:
Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 702:40-69
A collaborative trial was conducted to evaluate the possibility of integrating the rat-liver Comet assay into repeat-dose toxicity studies. Fourteen laboratories from Europe, Japan and the USA tested fifteen chemicals. Two chemicals had been previous