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Autor:
Ruth Freitag, Oscar-Werner Reif, Uwe T. Bornscheuer, Ralf Dr. Lausch, Uldrich Menge, Thomas Scheper, Fragiskos N. Kolisis
Publikováno v:
Biochimica et Biophysica Acta (BBA) - General Subjects. 1201:55-60
Commercial lipase (triacylglycerol lipase, EC 3.1.1.3) of Pseudomonas cepacia (Amano) has been purified to homogeneity by a single chromatography on phenyl Sepharose. The eluted lipase crystallized spontaneously at 4 degrees C in the eluent, containi