Výsledky vyhledávání - "Uitdehaag JCM"

The remote substrate binding subsite-6 in cyclodextrin-glycosyltransferase controls the transferase activity of the enzyme via an induced-fit mechanism

Autor: Leemhuis, H, Uitdehaag, JCM, Rozeboom, HJ, Dijkstra, BW, Dijkhuizen, L, Dijkstra, Bauke W.

Publikováno v: The Journal of Biological Chemistry, 277(2), 1113-1119. AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

Cyclodextrin-glycosyltransferase (CGTase) catalyzes the formation of alpha-, beta-, and gamma-cyclodextrins (cyclic alpha-(1,4)-linked oligosaccharides of 6, 7, or 8 glucose residues, respectively) from starch. Nine substrate binding subsites were ob

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4334e3ab6131a50e5a7eece454ebd26
https://doi.org/10.1074/jbc.M106667200

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Catalytic mechanism and product specificity of cyclodextrin glycosyltransferase, a prototypical transglycosylase from the alpha-amylase family

Autor: Uitdehaag, JCM, van der Veen, BA, Dijkhuizen, L, Dijkstra, BW

Publikováno v: Enzyme and Microbial Technology, 30(3):PII S0141-0229(01)00498-7, 295-304. ELSEVIER SCIENCE INC

The catalytic mechanism of cyclodextrin glycosyltransferase, a member of the a-amylase family, is reviewed. The focus is put on the bond cleavage mechanism, the nature of the transition state and of the covalent intermediate, and on the stereo-electr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=narcis______::0436a6ebe93df4c9983b0cfcf3337226
https://research.rug.nl/en/publications/06427b31-e23f-4e70-9153-8839ca7ab57f

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Cyclodextrin glycosyltransferase as a model enzyme to study the reaction mechanism of the alpha-amylase family

Autor: Uitdehaag, JCM, Dijkhuizen, L, Dijkstra, BW, Teeri, TT, Svensson, B, Gilbert, HJ, Feizi, T

Publikováno v: CARBOHYDRATE BIOENGINEERING: INTERDISCIPLINARY APPROACHES, 82-86
STARTPAGE=82;ENDPAGE=86;TITLE=CARBOHYDRATE BIOENGINEERING: INTERDISCIPLINARY APPROACHES

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=narcis______::86953379692847fa3051bc27bbcac654
https://research.rug.nl/en/publications/43c8eb9e-158a-4644-bb53-faa66a7cd54a

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Structures of maltohexaose and maltoheptaose bound at the donor sites of cyclodextrin glycosyltransferase give insight into the mechanisms of transglycosylation activity and cyclodextrin size specificity

Autor: Uitdehaag, JCM, van Alebeek, GJWM, Dijkhuizen, L, Dijkstra, BW, Alebeek, Gert-Jan W.M. van, Dijkstra, Bauke W.

Publikováno v: Biochemistry, 39(26), 7772-7780. AMER CHEMICAL SOC

The enzymes from the cl-amylase family all share a similar alpha-retaining catalytic mechanism but can have different reaction and product specificities. One family member, cyclodextrin glycosyltransferase (CGTase), has an uncommonly high transglycos

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::750ad7620d872414feeb9e6fcad8f6e7
https://pubmed.ncbi.nlm.nih.gov/10869182

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The cyclization mechanism of cyclodextrin glycosyltransferase (CGTase) as revealed by a gamma-cyclodextrin-CGTase complex at 1.8-angstrom resolution

Autor: Uitdehaag, JCM, Kalk, KH, Dijkhuizen, L, Dijkstra, BW

Publikováno v: The Journal of Biological Chemistry, 274(49), 34868-34876. AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

The enzyme cyclodextrin glycosyltransferase is closely related to alpha-amylases but has the unique ability to produce cyclodextrins (circular alpha(1-->4)-linked glucoses) from starch. To characterize this specificity we determined a 1.8-Angstrom st

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=narcis______::e04a0334ffb278fadfb9bfda271a4a74
https://research.rug.nl/en/publications/d4b4b5a0-5f36-4cc1-9282-3d25cd785153

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X-ray structures along the reaction pathway of cyclodextrin glycosyltransferase elucidate catalysis in the alpha-amylase family

Autor: Uitdehaag, JCM, Mosi, R, Kalk, KH, Dijkhuizen, L, Withers, SG, Dijkstra, BW

Publikováno v: Nature Structural Biology, 6(5), 432-436

Cyclodextrin glycosyltransferase (CGTase) is an enzyme of the alpha-amylase family, which uses a double displacement mechanism to process alpha-linked glucose polymers. We have determined two X-ray structures of CGTase complexes, one with an intact s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=narcis______::d41a5c7084fabf78d710ff67e0b2f689
https://research.rug.nl/en/publications/f95e3cae-fdaf-4322-b355-8eaf1e075070

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