Rapid and specific degradation of endogenous proteins in mouse models using auxin-inducible degrons

Autor: Gillian C Taylor, Lewis Macdonald, Jennifer Margaret Brisbane, Ersi Christodoulou, Lucy Scott, Alex von Kriegsheim, Janet Rossant, Bin Gu, Andrew J Wood

Publikováno v: MacDonald, L, Taylor, G, Brisbane, J, Christodoulou, E, Scott, L, von Kriegsheim, A, Rossant, J, Gu, B & Wood, A J 2022, ' Rapid and specific degradation of endogenous proteins in mouse models using auxin-inducible degrons ', eLIFE, vol. 11 . https://doi.org/10.7554/eLife.77987

Auxin-inducible degrons are a chemical genetic tool for targeted protein degradation and are widely used to study protein function in cultured mammalian cells. Here, we develop CRISPR-engineered mouse lines that enable rapid and highly specific degra

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd043f0d620aca83bc9390be51da816d
https://www.pure.ed.ac.uk/ws/files/285851556/elife_77987_v2.pdf

Cbx4 regulates the proliferation of thymic epithelial cells and thymus function

Autor: Liu, B., Liu, Y. F., Du, Y. R., Mardaryev, A. N., Yang, W., Chen, H., Xu, Z. M., Xu, C. Q., Zhang, X. R., Botchkarev, V. A., Zhang, Y., Xu, G. L.

Thymic epithelial cells (TECs) are the main component of the thymic stroma, which supports T-cell proliferation and repertoire selection. Here, we demonstrate that Cbx4, a Polycomb protein that is highly expressed in the thymic epithelium, has an ess

Externí odkaz: http://hdl.handle.net/10454/6068

UVR8-mediated inhibition of shade avoidance involves HFR1 stabilization in Arabidopsis

Autor: Tavridou, Eleni, Schmid-Siegert, Emanuel, Fankhauser, Christian, Ulm, Roman

Publikováno v: PLoS Genetics, Vol 16, Iss 5, p e1008797 (2020)
PLoS genetics, vol. 16, no. 5, pp. e1008797
PLoS Genetics
PLOS Genetics, Vol. 16, No 5 (2020) P. e1008797

Sun-loving plants perceive the proximity of potential light-competing neighboring plants as a reduction in the red:far-red ratio (R:FR), which elicits a suite of responses called the “shade avoidance syndrome” (SAS). Changes in R:FR are primarily

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::0545b92567db39a9ed60551075590f7f
https://doaj.org/article/0e9d7e2ea96b4a92a1377c80f65a3f8f

WWP2 regulates pathological cardiac fibrosis by modulating SMAD2 signaling

Autor: Cristina Beltrami, Owen J. L. Rackham, Costanza Emanueli, Nithya Devapragash, Wei-Ping Yu, Xi-Ming Sun, Stuart A. Cook, Norbert Hubner, Francesco Pesce, Ee Ling Heng, Elisabeth Tan, Aida Moreno-Moral, Enrique Lara-Pezzi, Gianni D Angelini, Enrico Petretto, Huimei Chen, Paul J.R. Barton, Nathan Harmston, Michal Pravenec, Sebastian Schafer, Maxime Rotival, Nicole Tee, Massimilano Mancini, Leanne E. Felkin, Leonardo Bottolo, Prashant K. Srivastava, Kirill Shkura

Publikováno v: Nature Communications
Nature Communications, 2019, 10 (1), pp.3616. ⟨10.1038/s41467-019-11551-9⟩
Nature Communications, Vol 10, Iss 1, Pp 1-19 (2019)
Chen, H, Moreno-Moral, A, Pesce, F, Devapragash, N, Mancini, M, Heng, E L, Rotival, M, Srivastava, P K, Harmston, N, Shkura, K, Rackham, O J L, Yu, W P, Sun, X M, Tee, N G Z, Tan, E L S, Barton, P J R, Felkin, L E, Lara-Pezzi, E, Angelini, G, Beltrami, C, Pravenec, M, Schafer, S, Bottolo, L, Hubner, N, Emanueli, C, Cook, S A & Petretto, E 2019, ' WWP2 regulates pathological cardiac fibrosis by modulating SMAD2 signalling ', Nature Communications, vol. 10, 3616 . https://doi.org/10.1038/s41467-019-11551-9
Nature Communications, Nature Publishing Group, 2019, 10 (1), pp.3616. ⟨10.1038/s41467-019-11551-9⟩
Repisalud
Instituto de Salud Carlos III (ISCIII)

Cardiac fibrosis is a final common pathology in inherited and acquired heart diseases that causes cardiac electrical and pump failure. Here, we use systems genetics to identify a pro-fibrotic gene network in the diseased heart and show that this netw

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b9a8eae768aaa77cac0482318b75243
https://doi.org/10.1038/s41467-019-11551-9

System-Wide Modulation of HECT E3 Ligases with Selective Ubiquitin Variant Probes

Autor: Emmanuel Lemichez, Brenda A. Schulman, Zhenyue Hao, Sachdev S. Sidhu, Nan Li, Ryan Murchie, Wei Zhang, Maria A. Sartori, Kuen-Phon Wu, Jason Moffat, John R. Walker, Avinash Persaud, Yi Sheng, Alban Ordureau, Jicheng Hu, Chong Jiang, Manjeet Mukherjee, Kevin R. Brown, Yufeng Tong, Yanjun Li, Junjie Chen, Anne Doye, Peter Y. Mercredi, Daniela Rotin, Hari B. Kamadurai, J. Wade Harper

Publikováno v: Molecular Cell
Molecular Cell, Elsevier, 2016, 62 (1), pp.121-136. ⟨10.1016/j.molcel.2016.02.005⟩

Comment in : A Billion Ubiquitin Variants to Probe and Modulate the UPS. [Mol Cell. 2016]; International audience; HECT-family E3 ligases ubiquitinate protein substrates to control virtually every eukaryotic process and are misregulated in numerous d

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c0423280b3710b384d45c649c83c073
https://doi.org/10.1016/j.molcel.2016.02.005