Feasibility of electroextraction as versatile sample preconcentration for fast and sensitive analysis of urine metabolites, demonstrated on acylcarnitines

Autor: Thomas Hankemeier, Jan van der Greef, U.R. Tjaden, Petrus W. Lindenburg

Publikováno v: ELECTROPHORESIS. 33:2987-2995

In this work, we demonstrate the applicability of electroextraction (EE) to urine metabolites. To investigate which urine metabolite classes are susceptible to EE, off-line EE experiments were carried out with a prototype device, in which urine metab

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::aae9ab86551d4c06964222a8343b0891
https://doi.org/10.1002/elps.201200276

Feasibility of electrodialysis as a fast and selective sample preparation method for the profiling of low-abundant peptides in biofluids

Autor: Robert van der Heijden, U.R. Tjaden, Jan van der Greef, Jeroen DeGroot, Jurre J. Kamphorst, Thomas Hankemeier

Publikováno v: Electrophoresis, 13, 30, 2284-2292

Considerable interest exists in endogenous peptides as potential biomarkers, since they act as signaling molecules and are formed by degradation of proteins. A crucial step in the profiling of these peptides is the sample preparation, which aims to e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ba140775006c49f96c1c9594a01723f
https://doi.org/10.1002/elps.200800500

Considerations for proteolytic labeling–optimization of18O incorporation and prohibition of back-exchange

Autor: U.R. Tjaden, Henricus F. Storms, Jan van der Greef, Robert van der Heijden

Publikováno v: Rapid Communications in Mass Spectrometry. 20:3491-3497

Proteolytic 18O labeling is a very powerful tool for differential analysis applied to proteome studies. However, it is a relatively new technique and the optimization of the labeling process still needs some attention. We found that the two-step post

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad2823e613b612d21ea6369470fb7417
https://doi.org/10.1002/rcm.2738

A generic assay for phosphate-consuming or -releasing enzymes coupled on-line to liquid chromatography for lead finding in natural products

Autor: J. van der Greef, T Schenk, Hubertus Irth, U.R. Tjaden, D.A. van Elswijk, Natalie M. G. M. Appels

Publikováno v: Schenk, T, Appels, N, van Elswijk, D A, Irth, H, Tjaden, U R & de Greef, J 2003, ' A generic assay for phosphate-consuming or-releasing enzymes coupled
to liquid chromatography for lead finding in natural products ', Analytical Biochemistry, vol. 316, no. 1, pp. 118-26 . https://doi.org/10.1016/S0003-2697(03)00038-1
Analytical Biochemistry, 316(1), 118-26. Academic Press Inc.

A generic continuous-flow assay for phosphate-consuming or -releasing enzymes coupled on-line to liquid chromatography (LC) has been developed. Operating the LC-biochemical assay in combination with mass spectrometry allows the fast detection and ide

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::733c370f696d0265a47aa4bf1c1b133a
https://doi.org/10.1016/s0003-2697(03)00038-1

Screening of natural products extracts for the presence of phosphodiesterase inhibitors using liquid chromatography coupled online to parallel biochemical detection and chemical characterization

Autor: G. J. Breel, Thomas Schenk, A. C. Hogenboom, S. van Den Berg, P. Koevoets, Hubertus Irth, J. van der Greef, U.R. Tjaden

Publikováno v: Schenk, T, Breel, G J, Koevoets, P, van den Berg, S A, Hogenboom, A C, Irth, H, Tjaden, U R & de Greef, J 2003, ' Screening of natural products extracts for the presence of phosphodiesterase inhibitors using liquid chromatography coupled online to parallel biochemical detection and chemical characterization ', Journal of Biomolecular Screening, vol. 8, no. 4, pp. 421-9 . https://doi.org/10.1177/1087057103255973
Journal of Biomolecular Screening, 8(4), 421-9. SAGE Publications Inc.

The ability to rapidly identify active compounds in a complex mixture (e.g., natural products extract) is still one of the major problems in natural products screening programs. An elegant way to overcome this problem is to separate the complex mixtu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b8946362835a28d1a4890bd8987f0e0
https://hdl.handle.net/1871/27694