Výsledky vyhledávání - "U. L. RajBhandary"

Expression and function of a human initiator tRNA gene in the yeast Saccharomyces cerevisiae

Publikováno v: Molecular and Cellular Biology. 10:4486-4494

We showed previously that the human initiator tRNA gene, in the context of its own 5'- and 3'-flanking sequences, was not expressed in Saccharomyces cerevisiae. Here we show that switching its 5'-flanking sequence with that of a yeast arginine tRNA g

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8d13ca91541cb53345957a3dfec44805
https://doi.org/10.1128/mcb.10.9.4486

Zobrazit plný text záznamu

Suppressor mutations in Escherichia coli methionyl-tRNA formyltransferase that compensate for the formylation defect of a mutant tRNA aminoacylated with lysine

Autor: Y, Li, V, Ramesh, D, Mangroo, C, Taneja, U L, RajBhandary

Publikováno v: Biochemistry. 39(27)

The specific formylation of initiator methionyl-tRNA by methionyl-tRNA formyltransferase (MTF) is important for the initiation of protein synthesis in eubacteria such as Escherichia coli. In addition to the determinants for formylation present in the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::26f9d1ecf4df10d538d7955ca2cada69
https://pubmed.ncbi.nlm.nih.gov/10891086

Zobrazit plný text záznamu

Intragenic suppression in tRNA: evidence for crosstalk between the D and the T stems

Autor: V, Ramesh, U, Varshney, U L, Rajbhandary

Publikováno v: RNA (New York, N.Y.). 3(11)

We showed previously that introduction of two of the three unique features of Escherichia coli initiator tRNA onto an elongator methionine tRNA conferred significant activity in initiation. Surprisingly, introduction also of the third unique feature,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::aacda7e151c787469457eddffe5a9d21
https://pubmed.ncbi.nlm.nih.gov/9409615

Zobrazit plný text záznamu

Role of the three consecutive G:C base pairs conserved in the anticodon stem of initiator tRNAs in initiation of protein synthesis in Escherichia coli

Autor: N, Mandal, D, Mangroo, J J, Dalluge, J A, McCloskey, U L, Rajbhandary

Publikováno v: RNA (New York, N.Y.). 2(5)

The three consecutive G:C base pairs, G29:C41, G30:C40, and G31:C39, are conserved in the anticodon stem of virtually all initiator tRNAs from eubacteria, eukaryotes, and archaebacteria. We show that these G:C base pairs are important for function of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::be3e2f0b95a0a6f8629f487025d83351
https://pubmed.ncbi.nlm.nih.gov/8665414

Zobrazit plný text záznamu

Mutants of Escherichia coli initiator tRNA defective in initiation. Effects of overproduction of methionyl-tRNA transformylase and the initiation factors IF2 and IF3

Autor: D, Mangroo, U L, RajBhandary

Publikováno v: The Journal of biological chemistry. 270(20)

We describe the effects of overproduction of methionyl-tRNA transformylase and initiation factors IF2 and IF3 on the activity, in vivo, of initiator tRNA mutants defective at specific steps of the initiation process in protein synthesis. The activity

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::3a865e88fc3a78be846676fa71688763
https://pubmed.ncbi.nlm.nih.gov/7538134

Zobrazit plný text záznamu

The role of nucleotides conserved in eukaryotic initiator methionine tRNAs in initiation of protein synthesis

Autor: H J, Drabkin, B, Helk, U L, RajBhandary

Publikováno v: The Journal of biological chemistry. 268(33)

Mutant human initiator tRNA genes carrying changes in each of the three features unique to eukaryotic initiator tRNAs have been constructed, and introduced into CV-1 monkey kidney cells using SV40 virus vectors. The mutant tRNA genes are expressed, a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::bd1e6e1a63ac6995728a6c6f0a5840bc
https://pubmed.ncbi.nlm.nih.gov/8227087

Zobrazit plný text záznamu

Saccharomyces cerevisiae cytoplasmic tyrosyl-tRNA synthetase gene. Isolation by complementation of a mutant Escherichia coli suppressor tRNA defective in aminoacylation and sequence analysis

Autor: C M, Chow, U L, RajBhandary

Publikováno v: The Journal of biological chemistry. 268(17)

Exploiting differences in tRNA recognition between prokaryotic and eukaryotic tyrosyl-tRNA synthetases (TyrRSs), we have isolated the gene for the cytoplasmic TyrRS of Saccharomyces cerevisiae by functional complementation in Escherichia coli of a mu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::c33e57cbb7be1b1c2917e4741e8f523d
https://pubmed.ncbi.nlm.nih.gov/8509419

Zobrazit plný text záznamu

Direct analysis of aminoacylation levels of tRNAs in vivo. Application to studying recognition of Escherichia coli initiator tRNA mutants by glutaminyl-tRNA synthetase

Autor: U, Varshney, C P, Lee, U L, RajBhandary

Publikováno v: The Journal of biological chemistry. 266(36)

We describe the use of a gel electrophoretic method for measuring the levels of aminoacylation in vivo of mutant Escherichia coli initiator tRNAs, which are substrates for E. coli glutaminyl-tRNA synthetase (GlnRS) due to an anticodon sequence change

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::5de86324052928dd736149bed16c612a
https://pubmed.ncbi.nlm.nih.gov/1761566

Zobrazit plný text záznamu

Structural and sequence elements important for recognition of Escherichia coli formylmethionine tRNA by methionyl-tRNA transformylase are clustered in the acceptor stem

Autor: C P, Lee, B L, Seong, U L, RajBhandary

Publikováno v: The Journal of biological chemistry. 266(27)

We show that the structure and/or sequence of the first three base pairs at the end of the amino acid acceptor stem of Escherichia coli initiator tRNA and the discriminator base 73 are important for its formylation by E. coli methionyl-tRNA transform

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::234d763101d9a821717f7da185fd4972
https://pubmed.ncbi.nlm.nih.gov/1917939

Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání