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Publikováno v:
Das Gesundheitswesen. 66:1-6
In recent years, Germany and Switzerland have changed national policies to recommend vaccination with IPV (inactivated polio vaccine) instead of OPV (oral polio vaccine) for protection against poliomyelitis. An all IPV-schedule in routine childhood p
Publikováno v:
Research in Microbiology. 149:145-154
Pathogenic strains of Escherichia coli producing verotoxins (VTs) have been recognized as a cause of human disease, and rapid and sensitive detection tests are urgently needed to ensure the safety of food, especially ground beef. We applied two neste
Autor:
U. Candrian, Rolf Meyer
Publikováno v:
LWT - Food Science and Technology. 29:1-9
Analysis of specific nucleic acids in food allows control laboratories to determine the presence or absence of certain ingredients in complex products or the identification of specific characteristics of single food components. These analyses are bas
Publikováno v:
Journal of AOAC INTERNATIONAL. 78:1531-1536
A polymerase chain reaction (PCR) method for direct detection of Salmonella spp. in whole-shell eggs is described. The method does not require isolating strains. Preenrichment, rapid DNA isolation, and a nested PCR system targeting the invA gene enab
Publikováno v:
Journal of AOAC INTERNATIONAL. 78:1542-1551
The polymerase chain reaction (PCR) technique was applied to meat species identification in marinated and heat-treated or fermented products and to the differentiation of closely related species. DNA was isolated from meat samples by using a DNA-bind
Autor:
U. Candrian
Publikováno v:
Journal of Microbiological Methods. 23:89-103
Many methods for the in vitro amplification of nucleic acids have been developed in recent years. Most of these methods are still at an experimental phase and only the polymerase chain reaction (PCR) is currently applied in food microbiology. The PCR
Autor:
C Höfelein, Jürg Lüthy, B Wegmüller, U. Candrian, E Köppel, C Niederhauser, Rolf Meyer, M. Allmann
Publikováno v:
Research in Microbiology. 146:85-97
The presence of pathogenic bacteria poses a serious problem in sustaining the safety of dairy products. Microbiological routine controls of these products make use of selective culture techniques. To detect pathogenic species, isolated colonies are c
Publikováno v:
Journal of Applied Bacteriology. 77:574-582
The polymerase chain reaction was used to obtain randomly-amplified polymorphic DNA (RAPD) profiles from Listeria spp. and enterobacteria. Eleven different oligonucleotides were evaluated. Only one, HR4 (19mer), generated reproducible and specific pr
Publikováno v:
Genome Research. 4:117-123
A major problem in the application of PCR is contamination with material amplified previously. Repeated PCRs result in the accumulation of intact and degraded amplicons and primer artifacts that can contaminate following amplification reactions. Post