Zobrazeno 1 - 10
of 26
pro vyhledávání: '"Tyler L, Dangerfield"'
Autor:
Grace N. Hibshman, Jack P. K. Bravo, Matthew M. Hooper, Tyler L. Dangerfield, Hongshan Zhang, Ilya J. Finkelstein, Kenneth A. Johnson, David W. Taylor
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-15 (2024)
Abstract CRISPR-Cas9 is a powerful tool for genome editing, but the strict requirement for an NGG protospacer-adjacent motif (PAM) sequence immediately next to the DNA target limits the number of editable genes. Recently developed Cas9 variants have
Externí odkaz:
https://doaj.org/article/bcc8918303d84f72875bedfc0ceaf526
Autor:
Evan A. Schwartz, Jack P. K. Bravo, Mohd Ahsan, Luis A. Macias, Caitlyn L. McCafferty, Tyler L. Dangerfield, Jada N. Walker, Jennifer S. Brodbelt, Giulia Palermo, Peter C. Fineran, Robert D. Fagerlund, David W. Taylor
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-14 (2024)
Abstract CRISPR-Cas are adaptive immune systems in bacteria and archaea that utilize CRISPR RNA-guided surveillance complexes to target complementary RNA or DNA for destruction1–5. Target RNA cleavage at regular intervals is characteristic of type
Externí odkaz:
https://doaj.org/article/df1f8e1485e54ad7bc5ecc11d0bc3d0e
Autor:
Simon d’Oelsnitz, Daniel J. Diaz, Wantae Kim, Daniel J. Acosta, Tyler L. Dangerfield, Mason W. Schechter, Matthew B. Minus, James R. Howard, Hannah Do, James M. Loy, Hal S. Alper, Y. Jessie Zhang, Andrew D. Ellington
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-14 (2024)
Abstract A major challenge to achieving industry-scale biomanufacturing of therapeutic alkaloids is the slow process of biocatalyst engineering. Amaryllidaceae alkaloids, such as the Alzheimer’s medication galantamine, are complex plant secondary m
Externí odkaz:
https://doaj.org/article/a590be639471443d8f68f7c532e9fc11
Publikováno v:
STAR Protocols, Vol 2, Iss 1, Pp 100357- (2021)
Summary: The RNA-dependent-RNA polymerase (RdRp) from SARS-CoV-2 is an important drug target because it is responsible for viral RNA genome replication. Efficient production of recombinant RdRp is important in screening antivirals to treat COVID-19.
Externí odkaz:
https://doaj.org/article/06e39987b45e4d21bbcf9316a380e0c8
Publikováno v:
iScience, Vol 23, Iss 12, Pp 101849- (2020)
Summary: COVID-19 is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and is currently being treated using Remdesivir, a nucleoside analog that inhibits the RNA-dependent-RNA polymerase (RdRp). However, the enzymatic mechani
Externí odkaz:
https://doaj.org/article/5d772ca53527407892309ffbeea7aab5
Publikováno v:
Nucleic Acids Research. 51:488-499
Loop-mediated isothermal amplification (LAMP) has proven to be easier to implement than PCR for point-of-care diagnostic tests. However, the underlying mechanism of LAMP is complicated and the kinetics of the major steps in LAMP have not been fully e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0bd790ce348cc216da9ab296dcccb670
https://doi.org/10.1093/nar/gkac1221
https://doi.org/10.1093/nar/gkac1221
Autor:
Jack P. K. Bravo, Mu-Sen Liu, Grace N. Hibshman, Tyler L. Dangerfield, Kyungseok Jung, Ryan S. McCool, Kenneth A. Johnson, David W. Taylor
Publikováno v:
Nature. 603:343-347
CRISPR–Cas9 as a programmable genome editing tool is hindered by off-target DNA cleavage1–4, and the underlying mechanisms by which Cas9 recognizes mismatches are poorly understood5–7. Although Cas9 variants with greater discrimination against
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::984fc4cf77a551b3116aa60d4788ab46
https://doi.org/10.1038/s41586-022-04470-1
https://doi.org/10.1038/s41586-022-04470-1
Publikováno v:
Methods in Enzymology ISBN: 9780443152764
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6a0421a04dba507d8ec73d6c43692e64
https://doi.org/10.1016/bs.mie.2023.03.018
https://doi.org/10.1016/bs.mie.2023.03.018
Autor:
Evan A. Schwartz, Jack P.K. Bravo, Luis A. Macias, Caitlyn L. McCafferty, Tyler L. Dangerfield, Jada N. Walker, Jennifer S. Brodbelt, Peter C. Fineran, Robert D. Fagerlund, David W. Taylor
CRISPR (Clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) systems are a type of adaptive immune response in bacteria and archaea that utilize crRNA (CRISPR RNA)-guided effector complexes to target complementary RNA or
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::669a5e2728c751135c36862a03e760eb
https://doi.org/10.1101/2022.06.13.496011
https://doi.org/10.1101/2022.06.13.496011
Publikováno v:
J Biol Chem
DNA polymerase from bacteriophage T7 undergoes large, substrate-induced conformational changes that are thought to account for high replication fidelity, but prior studies were adversely affected by mutations required to construct a Cys-lite variant
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aba5e269f906f3e5448697641e2cf35e
https://doi.org/10.1074/jbc.ra120.015557
https://doi.org/10.1074/jbc.ra120.015557