Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Tuula K. Torkkeli"'
Publikováno v:
Michel, P, Torkkeli, T, Karp, M & Oker-Blom, C 2001, ' Expression and purification of polyhistidine-tagged firefly luciferase in insect cells : A potential alternative for process scale-up ', Journal of Biotechnology, vol. 85, no. 1, pp. 49-56 . https://doi.org/10.1016/S0168-1656(00)00377-1
The coleopteran firefly, Photinus pyralis, luciferase was produced in lepidopteran Trichoplusia ni insect cells using a baculovirus expression vector. The recombinant protein was equipped with a polyhistidine affinity tag at the carboxyl terminus and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::40e2721ef0e5c57601b36d3a226b7fab
https://cris.vtt.fi/en/publications/b81a9102-3143-4a7e-8c0c-aff986d3bd52
https://cris.vtt.fi/en/publications/b81a9102-3143-4a7e-8c0c-aff986d3bd52
Publikováno v:
Biotechnology Letters. 15:277-282
The capacity to saccharify barley grain mash of Hormoconis resinae glucoamylase P produced by a heterologous host, Trichoderma reesei, was compared with that of Aspergillus niger glucoamylase. The results showed that the glucoamylase P secreted by T.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6f4a76f0d12ad167a39bc15a00f9d867
https://doi.org/10.1007/bf00128319
https://doi.org/10.1007/bf00128319
Autor:
Vesa Joutsjoki, Tuula K. Torkkeli
Publikováno v:
FEMS Microbiology Letters. 99:237-243
The glucoamylase P gene of the fungus Hormoconis resiane has been cloned and sequenced from a genomic library. The gene consists of a 2153-bp protein coding region including three introns. The usual number of introns in cloned fungal glucoamylase gen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4eb668e6e983eaa20fe61ebcd1a31823
https://doi.org/10.1111/j.1574-6968.1992.tb05574.x
https://doi.org/10.1111/j.1574-6968.1992.tb05574.x
Publikováno v:
Journal of biotechnology. 58(1)
An Aspergillus gene coding for a pH 2.5 acid phosphatase enzyme was successfully overexpressed in Trichoderma reesei under the strong main cellobiohydrolase I (cbh 1) promoter. The best transformants produced up to 240 times more of the acid phosphat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::424856d511f7cf48711f3ce47c9f01d9
https://pubmed.ncbi.nlm.nih.gov/9335175
https://pubmed.ncbi.nlm.nih.gov/9335175
Publikováno v:
FEMS microbiology letters. 112(3)
Secretion of the Hormoconis resinae glucoamylase P (GAMP) enzyme from Trichoderma reesei using either the natural N-terminal extension of the premature glucoamylase P or the cellobiohydrolase I (CBHI) signal peptide was examined. The expression condi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::761a39b5b15c26f0a74825f826bd9e62
https://pubmed.ncbi.nlm.nih.gov/8224791
https://pubmed.ncbi.nlm.nih.gov/8224791
Autor:
Tuula K. Torkkeli
Publikováno v:
Biochemical and Biophysical Research Communications. 97:559-565
Uterine cytosol and nuclear receptors were measured at various time intervals after one dose or two consecutive doses of progesterone and the receptor values compared with the course of induction of uteroglobin, a progesterone-regulated uterine prote
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e210b33a7d95dcd83044279b68c340f
https://doi.org/10.1016/0006-291x(80)90300-9
https://doi.org/10.1016/0006-291x(80)90300-9
Publikováno v:
International Journal of Andrology. 1:162-174
Progestational activity of natural and synthetic androgens was examined. All the androgens studied were able to induce uteroglobin synthesis in rabbit uterus. Some of the synthetic androgens were better inducers than progesterone itself. The data sug
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4d38f77f9c127615f58ecb163a8160fc
https://doi.org/10.1111/j.1365-2605.1978.tb00015.x
https://doi.org/10.1111/j.1365-2605.1978.tb00015.x
Autor:
Pirjo Lehtinen, Helena T. Kopu, Nisse Kalkkinen, Matti Korhola, Pirkko L. Liljestrom, Tuula K. Torkkeli, Hannele Ruohola
Publikováno v:
FEMS Microbiology Letters. 34:179-185
The Saccharomyces cerevisiae var. uvarum MEL1 gene, conferring production of α-galactosidase, has been cloned to study its expression. Increasing amounts of α-galactosidase protein and activity closely followed the induction of functional MEL1 tran
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::27d5e668a2b09b1222cd077bbd09e1a3
https://doi.org/10.1111/j.1574-6968.1986.tb01400.x
https://doi.org/10.1111/j.1574-6968.1986.tb01400.x
Publikováno v:
Molecular and Cellular Endocrinology. 9:101-118
A radioimmunoassay (RIA) for blastokinin from rabbit uterine fluid has been developed using antisera raised in male guinea pigs against purified blastokinin. [ 125 I]Iodoblastokinin was prepared with a chloramine-T method and the separation of bound
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::742cdef304ec8cbb3a09d6f9e841df9b
https://doi.org/10.1016/0303-7207(77)90050-8
https://doi.org/10.1016/0303-7207(77)90050-8
Publikováno v:
Biochemical Journal. 180:491-500
Investigations were conducted to quantify activity of uteroglobin mRNA and secretion of uteroglobin in rabbit uterus after administration of progesterone and 5alpha-dihydrotestosterone, either alone or concomitantly with oestradiol-17beta and tamoxif
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cab457f9bc66269fd62aea396964e999
https://doi.org/10.1042/bj1800491
https://doi.org/10.1042/bj1800491