Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Tuomas P.J. Knowles"'
Autor:
Marc Emmenegger, Roland Worth, Sebastian Fiedler, Sean R.A. Devenish, Tuomas P.J. Knowles, Adriano Aguzzi
Publikováno v:
STAR Protocols, Vol 4, Iss 1, Pp 102095- (2023)
Summary: Conventional methods of measuring affinity are limited by artificial immobilization, large sample volumes, and homogeneous solutions. This protocol describes microfluidic antibody affinity profiling on complex human samples in solution to ob
Externí odkaz:
https://doaj.org/article/a1a74bf054e44e15908074e6ad2294c6
Autor:
Marc Emmenegger, Elena De Cecco, David Lamparter, Raphaël P.B. Jacquat, Julien Riou, Dominik Menges, Tala Ballouz, Daniel Ebner, Matthias M. Schneider, Itzel Condado Morales, Berre Doğançay, Jingjing Guo, Anne Wiedmer, Julie Domange, Marigona Imeri, Rita Moos, Chryssa Zografou, Leyla Batkitar, Lidia Madrigal, Dezirae Schneider, Chiara Trevisan, Andres Gonzalez-Guerra, Alessandra Carrella, Irina L. Dubach, Catherine K. Xu, Georg Meisl, Vasilis Kosmoliaptsis, Tomas Malinauskas, Nicola Burgess-Brown, Ray Owens, Stephanie Hatch, Juthathip Mongkolsapaya, Gavin R. Screaton, Katharina Schubert, John D. Huck, Feimei Liu, Florence Pojer, Kelvin Lau, David Hacker, Elsbeth Probst-Müller, Carlo Cervia, Jakob Nilsson, Onur Boyman, Lanja Saleh, Katharina Spanaus, Arnold von Eckardstein, Dominik J. Schaer, Nenad Ban, Ching-Ju Tsai, Jacopo Marino, Gebhard F.X. Schertler, Nadine Ebert, Volker Thiel, Jochen Gottschalk, Beat M. Frey, Regina R. Reimann, Simone Hornemann, Aaron M. Ring, Tuomas P.J. Knowles, Milo A. Puhan, Christian L. Althaus, Ioannis Xenarios, David I. Stuart, Adriano Aguzzi
Publikováno v:
iScience, Vol 26, Iss 2, Pp 105928- (2023)
Summary: Effective public health measures against SARS-CoV-2 require granular knowledge of population-level immune responses. We developed a Tripartite Automated Blood Immunoassay (TRABI) to assess the IgG response against three SARS-CoV-2 proteins.
Externí odkaz:
https://doaj.org/article/bea10cd3141c4742a1c82d8153f04eb2
Autor:
Hongjia Zhu, Lianne W.Y. Roode, Aled J. Parry, Nadia A. Erkamp, Marc Rodriguez-Garcia, Masako Narita, Yi Shen, Yangteng Ou, Zenon Toprakcioglu, Masashi Narita, Tuomas P.J. Knowles
Publikováno v:
Advanced NanoBiomed Research, Vol 2, Iss 12, Pp n/a-n/a (2022)
Multicellular cancer spheroids (MCSs) have emerged as a promising in vitro model that recaptures many features of solid tumours in vivo. To generate cancer spheroids, cells are encapsulated in microgels with high throughput. While the biophysical pro
Externí odkaz:
https://doaj.org/article/369ea35e844a417f8d8542434b7c0f1a
Autor:
Marc Emmenegger, Sebastian Fiedler, Silvio D. Brugger, Sean R.A. Devenish, Alexey S. Morgunov, Alison Ilsley, Francesco Ricci, Anisa Y. Malik, Thomas Scheier, Leyla Batkitar, Lidia Madrigal, Marco Rossi, Georg Meisl, Andrew K. Lynn, Lanja Saleh, Arnold von Eckardstein, Tuomas P.J. Knowles, Adriano Aguzzi
Publikováno v:
iScience, Vol 25, Iss 8, Pp 104766- (2022)
Summary: The B.1.1.529 (omicron) variant has rapidly supplanted most other SARS-CoV-2 variants. Using microfluidics-based antibody affinity profiling (MAAP), we have characterized affinity and IgG concentration in the plasma of 39 individuals with mu
Externí odkaz:
https://doaj.org/article/fde6b01a90544d528df0a5aabe263227
Autor:
Alyssa Miller, Sean Chia, Ewa Klimont, Tuomas P.J. Knowles, Michele Vendruscolo, Francesco Simone Ruggeri
Many proteins self-assemble to form amyloid fibrils, which are highly organized structures stabilized by a characteristic cross-β network of hydrogen bonds. This process underlies a variety of human diseases, and can be exploited to develop versatil
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6ad7686460c130849e79a1e43ed324d2
https://doi.org/10.1101/2023.04.11.536374
https://doi.org/10.1101/2023.04.11.536374
Autor:
Nadia A. Erkamp, Marc Oeller, Tomas Sneideris, Hannes Ausserwoger, Aviad Levin, Timothy J. Welsh, Runzhang Qi, Daoyuan Qian, Nikolai Lorenzen, Hongjia Zhu, Pietro Sormanni, Michele Vendruscolo, Tuomas P.J. Knowles
Funder: Novo Nordisk
Funder: Frances and Augustus Newman Foundation
Funder: China Scholarship Council
Funder: University of Cambridge
Funder: AstraZeneca
Protein-based biologics are highly suitable for drug development as the
Funder: Frances and Augustus Newman Foundation
Funder: China Scholarship Council
Funder: University of Cambridge
Funder: AstraZeneca
Protein-based biologics are highly suitable for drug development as the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b83a26aa4e5781f0f1f528c8a53e9c65
https://www.repository.cam.ac.uk/handle/1810/348848
https://www.repository.cam.ac.uk/handle/1810/348848
Autor:
Jonathon Nixon-Abell, Francesco S. Ruggeri, Seema Qamar, Therese W. Herling, Magdalena A. Czekalska, Yi Shen, Guozhen Wang, Christopher King, Michael S. Fernandopulle, Tomas Sneideris, Joseph L. Watson, Visakh V.S. Pillai, William Meadows, James W. Henderson, Joseph E. Chambers, Jane L. Wagstaff, Sioned H. Williams, Helena Coyle, Yuqian Lu, Shuyuan Zhang, Stefan J. Marciniak, Stefan M.V. Freund, Emmanuel Derivery, Michael E. Ward, Michele Vendruscolo, Tuomas P.J. Knowles, Peter St George-Hyslop
Publikováno v:
bioRxiv
SUMMARYPhase transitions of cellular proteins and lipids play a key role in governing the organisation and coordination of intracellular biology. The frequent juxtaposition of proteinaceous biomolecular condensates to cellular membranes raises the in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33defe860df5665cc889d6dd86e39881
https://europepmc.org/articles/PMC10055329/
https://europepmc.org/articles/PMC10055329/
Autor:
Danny D. Sahtoe, Ewa A. Andrzejewska, Hannah L. Han, Enrico Rennella, Matthias M. Schneider, Georg Meisl, Maggie Ahlrichs, Justin Decarreau, Hannah Nguyen, Alex Kang, Paul Levine, Mila Lamb, Xinting Li, Asim K. Bera, Lewis E. Kay, Tuomas P.J. Knowles, David Baker
Segments of proteins with β-strand propensity can self associate to form amyloid fibrils associated with many diseases. These regions often adopt alternative structures in their folded states, or are intrinsically disordered in solution, making it d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2e438a9b349f9863535f67448f91f321
https://doi.org/10.1101/2023.01.13.523785
https://doi.org/10.1101/2023.01.13.523785
Autor:
Nadia A. Erkamp, Marc Oeller, Tomas Sneideris, Hannes Ausserwӧger, Aviad Levin, Timothy Welsh, Runzhang Qi, Daoyuan Qian, Hongjia Zhu, Pietro Sormanni, Michele Vendruscolo, Tuomas P.J. Knowles
Protein-based biologics are highly suitable for drug development, as they exhibit low toxicity and high specificity for their targets. However, for therapeutic applications, biologics must often be formulated to very high concentrations, making insuf
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::47198071525333691b212eb3ceeaebda
https://doi.org/10.1101/2022.10.21.513267
https://doi.org/10.1101/2022.10.21.513267
Autor:
Matthias M. Schneider, Tom Scheidt, Ashley J. Priddey, Catherine K. Xu, Mengsha Hu, Georg Meisl, Sean R.A. Devenish, Christopher M. Dobson, Vasilis Kosmoliaptsis, Tuomas P.J. Knowles
Publikováno v:
Biosensors and Bioelectronics. 228:115196
Antibody profiling is a fundamental component of understanding the humoral response in a wide range of disease areas. Most currently used approaches operate by capturing antibodies onto functionalised surfaces. Such measurements of surface binding ar