Protocol for Establishing Mouse Embryonic Stem Cells to Study Histone Inheritance Pattern at Single-Cell Resolution

Autor: Binbin Ma, Tung-Jui Trieu, Shukry J. Habib, Xin Chen

Publikováno v: STAR Protocols, Vol 1, Iss 3, Pp 100178- (2020)

Summary: Asymmetric histone inheritance can regulate cell-fate determination in Drosophila male germline stem cells. However, it remains elusive how this mechanism may be used in mammalian system. Recently, we show mouse embryonic stem cells (mESCs)

Externí odkaz: https://doaj.org/article/711ae6d721174f59b4bb762ef112dc07

Specialized cytonemes induce self-organization of stem cells

Autor: Clare L. Garcin, Tung-Jui Trieu, James L. A. Szczerkowski, Joshua Reeves, Shukry J. Habib, Sergi Junyent

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Junyent, S, Garcin, C L, Szczerkowski, J L A, Trieu, T-J, Reeves, J & Habib, S J 2020, ' Specialized cytonemes induce self-organization of stem cells ', Proceedings of National Academy of Sciences USA, vol. 117, no. 13, pp. 7236-7244 . https://doi.org/10.1073/pnas.1920837117

Significance Many questions about how stem cells communicate with neighboring cells and self-organize to initiate tissue formation remain unanswered. We uncovered mechanisms employed by embryonic stem cells (ESCs) and trophoblast stem cells (TSCs) to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ec937e62bbcfa1617272cda691135bc
http://europepmc.org/articles/PMC7132109

Protocol for Establishing Mouse Embryonic Stem Cells to Study Histone Inheritance Pattern at Single-Cell Resolution

Autor: Xin Chen, Binbin Ma, Shukry J. Habib, Tung-Jui Trieu

Publikováno v: STAR Protocols, Vol 1, Iss 3, Pp 100178-(2020)
STAR Protocols

Summary Asymmetric histone inheritance can regulate cell-fate determination in Drosophila male germline stem cells. However, it remains elusive how this mechanism may be used in mammalian system. Recently, we show mouse embryonic stem cells (mESCs) w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7372cd00ba90f00ce13ed29141f84c89
http://www.sciencedirect.com/science/article/pii/S2666166720301659

Differential Histone Distribution Patterns in Induced Asymmetrically Dividing Mouse Embryonic Stem Cells

Autor: Qingsong Tang, Ji-Long Liu, Ji Cheng, Shukry J. Habib, Binbin Ma, Tung-Jui Trieu, Keji Zhao, Shuang Zhou, Jing Xie, Xin Chen

Publikováno v: Cell Reports, Vol 32, Iss 6, Pp 108003-(2020)
Cell Rep

Summary: Wnt3a-coated beads can induce asymmetric divisions of mouse embryonic stem cells (mESCs), resulting in one self-renewed mESC and one differentiating epiblast stem cell. This provides an opportunity for studying histone inheritance pattern at

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd696e4abf8cd26ef9d6f3ea75016eab
http://www.sciencedirect.com/science/article/pii/S2211124720309888

Wnt- and Glutamate-receptors orchestrate stem cell dynamics and asymmetric cell division

Autor: Shukry J. Habib, Joshua Reeves, Sergi Junyent, Tung-Jui Trieu, Jethro Lundie-Brown, Matthew Wilson, James L. A. Szczerkowski, Clare L. Garcin

Publikováno v: eLife
eLife, Vol 10 (2021)

The Wnt-pathway is part of a signalling network that regulates many aspects of cell biology. Recently we discovered crosstalk between AMPA/Kainate-type ionotropic glutamate receptors (iGluRs) and the Wnt-pathway during the initial Wnt3a-interaction a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fcaee5103180d5a6570390069de10e38
https://doi.org/10.1101/2020.06.16.154609