Analyses of pre-steady-state kinetics and isotope effects of the γ-elimination reaction catalyzed by Citrobacter freundii methionine γ-lyase.

Autor: Kuznetsova AA; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk, 630090, Russia., Faleev NG; Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Moscow, 119991, Russia., Morozova EA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia., Anufrieva NV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia., Gogoleva OI; Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Moscow, 119991, Russia., Tsvetikova MA; Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Moscow, 119991, Russia., Fedorova OS; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk, 630090, Russia., Demidkina TV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia. Electronic address: tvdemidkina@yandex.ru., Kuznetsov NA; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk, 630090, Russia. Electronic address: nikita.kuznetsov@niboch.nsc.ru.

Publikováno v: Biochimie [Biochimie] 2022 Oct; Vol. 201, pp. 157-167. Date of Electronic Publication: 2022 Jun 09.

Serine 51 residue of Citrobacter freundii tyrosine phenol-lyase assists in C-α-proton abstraction and transfer in the reaction with substrate.

Autor: Barbolina MV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Vavilov Street 32, Russia., Kulikova VV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Vavilov Street 32, Russia., Tsvetikova MA; Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Moscow 119991, Vavilov Street 28, Russia., Anufrieva NV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Vavilov Street 32, Russia., Revtovich SV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Vavilov Street 32, Russia., Phillips RS; Department of Chemistry and Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA., Gollnick PD; Department of Biological Sciences, State University of New York at Buffalo, Buffalo, NY 14260, USA., Demidkina TV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Vavilov Street 32, Russia. Electronic address: tvd@eimb.ru., Faleev NG; Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Moscow 119991, Vavilov Street 28, Russia. Electronic address: ngfal@ineos.ac.ru.

Publikováno v: Biochimie [Biochimie] 2018 Apr; Vol. 147, pp. 63-69. Date of Electronic Publication: 2017 Nov 26.

A straightforward kinetic evidence for coexistence of "induced fit" and "selected fit" in the reaction mechanism of a mutant tryptophan indole lyase Y72F from Proteus vulgaris.

Autor: Faleev NG; Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Moscow 119991, Russia. Electronic address: ngfal@ineos.ac.ru., Zakomirdina LN; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia., Vorob'ev MM; Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Moscow 119991, Russia., Tsvetikova MA; Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Moscow 119991, Russia., Gogoleva OI; Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Moscow 119991, Russia., Demidkina TV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia., Phillips RS; Department of Chemistry, University of Georgia, Athens, GA 30602, USA; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA.

Publikováno v: Biochimica et biophysica acta [Biochim Biophys Acta] 2014 Oct; Vol. 1844 (10), pp. 1860-7. Date of Electronic Publication: 2014 Jul 30.

Methionine gamma-lyase: mechanistic deductions from the kinetic pH-effects. The role of the ionic state of a substrate in the enzymatic activity.

Autor: Faleev NG; Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Moscow 119991, Russian Federation. ngfal@ineos.ac.ru, Alferov KV, Tsvetikova MA, Morozova EA, Revtovich SV, Khurs EN, Vorob'ev MM, Phillips RS, Demidkina TV, Khomutov RM

Publikováno v: Biochimica et biophysica acta [Biochim Biophys Acta] 2009 Oct; Vol. 1794 (10), pp. 1414-20. Date of Electronic Publication: 2009 Jun 06.

Integrating enzyme evolution and high-throughput screening for efficient biosynthesis of l-DOPA.

Autor: Zeng, Weizhu^1,2 (AUTHOR), Xu, Bingbing^1,2,3 (AUTHOR), Du, Guocheng^1,4 (AUTHOR), Chen, Jian^1,2,3 (AUTHOR), Zhou, Jingwen^1,2,3 (AUTHOR) zhoujw1982@jiangnan.edu.cn

Publikováno v: Journal of Industrial Microbiology & Biotechnology. Dec2019, Vol. 46 Issue 12, p1631-1641. 11p. 1 Diagram, 3 Charts, 3 Graphs.