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pro vyhledávání: '"Tsuyoshi Yamanoue"'
Autor:
Naoki Eisaki, Takaharu Sakiyama, Tsuyoshi Yamanoue, Koshiki Mino, Katsuhiro Ohno, Asahi Matsuyama, Kazuhiro Nakanishi
Publikováno v:
Biotechnology Letters. 23:2051-2055
β-(Pyrazol-1-yl)-l-alanine (β-PA) was produced from l-serine and pyrazol using recombinant Escherichia coli cells expressing serine acetyltransferase and O-acetylserine sulfhydrylase-A. The amount of β-PA increased with increasing l-serine concent
Autor:
Naoki Eisaki, Koshiki Mino, Tsuyoshi Yamanoue, Asahi Matsuyama, Takaharu Sakiyama, Kazuhiro Nakanishi
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 64:1628-1640
Some properties and kinetics of the free and bound serine acetyltransferases (SATs) and O-acetylserine sulfhydrylase-As (OASS-As) from Escherichia coli were investigated. In some cases, SATdeltaC20, deleting 20 amino acid residues from the C-terminus
Autor:
Kazuhiro Nakanishi, Tsuyoshi Yamanoue, Naoki Eisaki, Koshiki Mino, Takaharu Sakiyama, Asahi Matsuyama
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 63:168-179
Incubation of serine acetyltransferase (SAT) from Escherichia coli at 25 degrees C in the absence of protease inhibitors yielded a truncated SAT. The truncated SAT was much less sensitive to feedback inhibition than the wild-type SAT. Analyses of the