Zobrazeno 1 - 10
of 96
pro vyhledávání: '"Tsuyoshi Muramatsu"'
Publikováno v:
Bioscience, biotechnology, and biochemistry. 57(7)
Poly(α-L-guluronate)lyase, which depolymerizes polyguluronate of alginate, was purified from the culture medium of a marine bacterium isolated from the intestine contents of a red sea bream, Pagrus major. The enzyme was homogeneous as judged by poly
Autor:
Yoko Takahashi, Nobue Takamatsu, Hiroshi Fujisaki, Tsuyoshi Muramatsu, Yasuhiro Ishibashi, Takuji Nakashima, Nakamichi Takahiro
Publikováno v:
Environmental Engineering Science. 27:993-999
Solubilization of sewage sludge by thermophilic bacteria isolated from a hot spring was investigated as a pretreatment for the methane fermentation process. The bacterial strain, designated strain MU3, was grown at an optimum pH of 7.5–8.0 and opti
Publikováno v:
Journal of Molecular Biology. 345:1111-1118
The crystal structure of alginate (poly alpha-l-guluronate) lyase from Corynebacterium sp. (ALY-1) was determined at 1.2A resolution using the MAD method and bromide ions. The structure of ALY-1 is abundant in beta-strands and has a deep cleft, simil
Autor:
Maki Kurachi, Takuji Nakashima, Kenichi Yamaguchi, Tatsuya Oda, Chihiro Miyajima, Yoshiko Iwamoto, Tsuyoshi Muramatsu
Publikováno v:
Journal of Infection and Chemotherapy. 11:199-203
We compared the abilities of alginate polymers having different molecular sizes and compositions to induce the secretion of tumor necrosis factor (TNF)-alpha in RAW264.7 cells. The molecular sizes and alpha-L-guluronate/beta-D-mannuronate (M/G) ratio
Publikováno v:
Journal of Protein Chemistry. 21:455-463
The entire amino acid sequence of bifunctional alginate lyase from Pseudoalteromonas sp. strain No. 272 were determined by two approaches, Edman degradation of the peptides obtained from protease digestion of the enzyme protein and analysis of PCR pr
Publikováno v:
Journal of Biochemistry. 132:441-449
We have found that a brefeldin A (BFA)-resistant mutant cell line derived from Vero cells (BER-40) is highly resistant to ricin-induced apoptosis as compared with parental Vero cells. In BER-40 cells, all apoptotic events caused by ricin including cy
Publikováno v:
Toxicology Letters. 132:141-151
We found that treatment of U937 cells with ZnCl 2 resulted in marked inhibition of ricin-induced DNA fragmentation and nuclear morphological change. Zn 2+ also completely inhibited the activation of caspase-3-, caspase-6-, and caspase-9-like protease
Publikováno v:
Aquatic Toxicology. 56:191-196
Red tides of Heterocapsa circularisquama (H. circularisquama), recently identified as a novel species of dinoflagellate, have frequently caused mass mortality of several species of bivalves in Japan, while no harmful effects of this flagellate on fis
Comparison of the Apoptosis-inducing Abilities of Various Protein Synthesis Inhibitors in U937 Cells
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 66:835-839
We compared the abilities of ricin, diphtheria toxin, cycloheximide, and anisomycin to induce apoptosis, using human myeloid leukemia U937 cells at the concentration of each toxin at which almost complete protein synthesis inhibition was attained wit
Publikováno v:
Fisheries Science. 67:956-964
Poly(α-L-guluronate)lyase, as one of alginate lyases, was purified from the culture medium of a marine bacterium, Pseudomonas sp. strain F6, to an electrophoretically homogeneous state. The enzyme was shown to have a molecular mass of 36 kDa by sodi