Zobrazeno 1 - 10
of 196
pro vyhledávání: '"Tsuneo Okuyama"'
Publikováno v:
PLoS ONE, Vol 14, Iss 9, p e0222199 (2019)
Infectious virus purification techniques are important for vaccine development and gene therapy applications. However, the standardized one-step purification technique using ceramic hydroxyapatite (CHAp) has proven unsuitable for poliovirus. Therefor
Externí odkaz:
https://doaj.org/article/c678c725bb00461697aa51c32c00a9fc
Publikováno v:
PLoS ONE, Vol 8, Iss 1, p e53893 (2013)
Although ceramic hydroxyapatite (HAp) chromatography has been used as an alternative method ultracentrifugation for the production of vaccines, the mechanism of virus separation is still obscure. In order to begin to understand the mechanisms of viru
Externí odkaz:
https://doaj.org/article/6b3066d8c3694d5baa8979e1f5c840c4
Autor:
Ken Sugo, Tsuneo Okuyama
Publikováno v:
Separation Science and Technology. 53:1572-1579
We studied the one-step purification of endotoxin by hydroxyapatite chromatography using total lipids extracted from Escherichia coli by the Bligh and Dyer method. High endotoxic activity was detec...
Publikováno v:
PLoS ONE
PLoS ONE, Vol 14, Iss 9, p e0222199 (2019)
PLoS ONE, Vol 14, Iss 9, p e0222199 (2019)
Infectious virus purification techniques are important for vaccine development and gene therapy applications. However, the standardized one-step purification technique using ceramic hydroxyapatite (CHAp) has proven unsuitable for poliovirus. Therefor
Publikováno v:
Separation and Purification Technology. 103:161-166
The preparation method for the surface zinc-modified hydroxyapatite particles that we developed as immobilized metal ion affinity chromatography medium, required decantation process to remove the excess zinc ion. Therefore, large-scale production was
Publikováno v:
Hybridoma. 31:68-71
Antibody purification using proteins A and G has been a standard method for research and industrial processes. The conventional method, however, includes a three-step process, including buffer exchange, before chromatography. In addition, proteins A
Publikováno v:
World Journal of Vaccines. :155-160
Dengue virus type 2 ThNH7/93 retained infectious activity after purification by ceramic hydroxyapatite chromatogra-phy. Dengue virus type 2 culture fluid was loaded onto the ceramic hydroxyapatite column and eluted with a linear gradient of sodium ph
Publikováno v:
Separation Science and Technology. 46:1306-1312
Previously, we developed polyethylenimine-coated hydroxyapatite as a chromatography medium. This report describes a simple, safe, productive, and improved preparation method and reports further chromatographic properties. The improved polyethylenimin
Autor:
Ken Sugo, Shintaro Kobayashi, Katsumi Kawamura, Masahiro Tomita, Yae Kurosawa, Tsuneo Okuyama, Tomohiko Yoshitake
Publikováno v:
Separation and Purification Technology. 76:432-435
Histidine-tagged proteins have been purified with immobilized nickel affinity chromatography. This method, however, bears some serious disadvantages, including carcinogenicity and potential leakage of the nickel and the handling and disposal costs. W
Autor:
Shingo Rikimra, Katsumi Kawamura, Yukiko Murakami, Tsuneo Okuyama, Tetsuro Ogawa, Ken Sugo, Hirano Masahiro
Publikováno v:
Journal of Liquid Chromatography & Related Technologies. 32:407-417
Hydroxyapatite (HAp-CHT TypeII 40 µm) was coated with a stable layer of polyethylenimine (PEI). The resulting material was useful in column chromatography of acidic materials. A mixture of AMP, ADP, ATP, and adenosine could be resolved readily. The