NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals.

Autor: Mougios N; Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, Göttingen, Germany.; Center for Biostructural Imaging of Neurodegeneration (BIN), University of Göttingen Medical Center, Göttingen, Germany., Cotroneo ER; Institute for Organic and Biomolecular Chemistry, University of Göttingen, Göttingen, Germany., Imse N; Institute for Organic and Biomolecular Chemistry, University of Göttingen, Göttingen, Germany., Setzke J; Center for Biostructural Imaging of Neurodegeneration (BIN), University of Göttingen Medical Center, Göttingen, Germany., Rizzoli SO; Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, Göttingen, Germany.; Cluster of Excellence 'Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells' (MBExC), University of Göttingen, Göttingen, Germany., Simeth NA; Institute for Organic and Biomolecular Chemistry, University of Göttingen, Göttingen, Germany.; Cluster of Excellence 'Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells' (MBExC), University of Göttingen, Göttingen, Germany., Tsukanov R; III. Institute of Physics - Biophysics, Georg August University, Göttingen, Germany., Opazo F; Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, Göttingen, Germany. fopazo@gwdg.de.; Center for Biostructural Imaging of Neurodegeneration (BIN), University of Göttingen Medical Center, Göttingen, Germany. fopazo@gwdg.de.; NanoTag Biotechnologies GmbH, Göttingen, Germany. fopazo@gwdg.de.

Publikováno v: Nature communications [Nat Commun] 2024 Oct 10; Vol. 15 (1), pp. 8771. Date of Electronic Publication: 2024 Oct 10.

A Versatile Synaptotagmin-1 Nanobody Provides Perturbation-Free Live Synaptic Imaging And Low Linkage-Error in Super-Resolution Microscopy.

Autor: Queiroz Zetune Villa Real K; Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073, Göttingen, Germany.; Center for Biostructural Imaging of Neurodegeneration (BIN), University Medical Center Göttingen, 37075, Göttingen, Germany., Mougios N; Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073, Göttingen, Germany.; Center for Biostructural Imaging of Neurodegeneration (BIN), University Medical Center Göttingen, 37075, Göttingen, Germany., Rehm R; Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073, Göttingen, Germany., Sograte-Idrissi S; Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073, Göttingen, Germany.; Center for Biostructural Imaging of Neurodegeneration (BIN), University Medical Center Göttingen, 37075, Göttingen, Germany., Albert L; Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073, Göttingen, Germany.; Center for Biostructural Imaging of Neurodegeneration (BIN), University Medical Center Göttingen, 37075, Göttingen, Germany., Rahimi AM; Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073, Göttingen, Germany.; Center for Biostructural Imaging of Neurodegeneration (BIN), University Medical Center Göttingen, 37075, Göttingen, Germany., Maidorn M; Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073, Göttingen, Germany.; Center for Biostructural Imaging of Neurodegeneration (BIN), University Medical Center Göttingen, 37075, Göttingen, Germany., Hentze J; Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073, Göttingen, Germany.; Center for Biostructural Imaging of Neurodegeneration (BIN), University Medical Center Göttingen, 37075, Göttingen, Germany., Martínez-Carranza M; Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Stockholm, SE-10691, Sweden., Hosseini H; Research Group Neuromodulatory Networks, Leibniz Institute for Neurobiology, 39118, Magdeburg, Germany., Saal KA; Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073, Göttingen, Germany., Oleksiievets N; III. Institute of Physics - Biophysics, Georg August University, 37077, Göttingen, Germany., Prigge M; Research Group Neuromodulatory Networks, Leibniz Institute for Neurobiology, 39118, Magdeburg, Germany.; Center for Behavioral Brain Sciences, 39118, Magdeburg, Germany., Tsukanov R; III. Institute of Physics - Biophysics, Georg August University, 37077, Göttingen, Germany., Stenmark P; Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Stockholm, SE-10691, Sweden., Fornasiero EF; Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073, Göttingen, Germany., Opazo F; Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073, Göttingen, Germany.; Center for Biostructural Imaging of Neurodegeneration (BIN), University Medical Center Göttingen, 37075, Göttingen, Germany.; NanoTag Biotechnologies GmbH, 37079, Göttingen, Germany.

Publikováno v: Small methods [Small Methods] 2023 Oct; Vol. 7 (10), pp. e2300218. Date of Electronic Publication: 2023 Jul 08.

Single-Molecule Fluorescence Lifetime Imaging Using Wide-Field and Confocal-Laser Scanning Microscopy: A Comparative Analysis.

Autor: Oleksiievets N; III. Institute of Physics - Biophysics, Georg August University, 37077 Göttingen, Germany., Mathew C; Laboratory of Supramolecular Chemistry, EPFL SB ISIC LCS, BCH 3307, CH-1015 Lausanne, Switzerland., Thiele JC; III. Institute of Physics - Biophysics, Georg August University, 37077 Göttingen, Germany., Gallea JI; III. Institute of Physics - Biophysics, Georg August University, 37077 Göttingen, Germany., Nevskyi O; III. Institute of Physics - Biophysics, Georg August University, 37077 Göttingen, Germany., Gregor I; III. Institute of Physics - Biophysics, Georg August University, 37077 Göttingen, Germany., Weber A; Combinatorial NeuroImaging Core Facility, Leibniz Institute for Neurobiology, Brenneckestraße 6, 39118 Magdeburg, Germany., Tsukanov R; III. Institute of Physics - Biophysics, Georg August University, 37077 Göttingen, Germany., Enderlein J; III. Institute of Physics - Biophysics, Georg August University, 37077 Göttingen, Germany.; Cluster of Excellence 'Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells' (MBExC), Georg August University, 37077 Göttingen, Germany.

Publikováno v: Nano letters [Nano Lett] 2022 Aug 10; Vol. 22 (15), pp. 6454-6461. Date of Electronic Publication: 2022 Jul 06.

Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells.

Autor: Oleksiievets N; III. Institute of Physics - Biophysics, Georg August University, 37077, Göttingen, Germany., Sargsyan Y; Department of Child and Adolescent Health, University Medical Center Göttingen, 37073, Göttingen, Germany., Thiele JC; III. Institute of Physics - Biophysics, Georg August University, 37077, Göttingen, Germany., Mougios N; Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073, Göttingen, Germany.; Center for Biostructural Imaging of Neurodegeneration (BIN), University Medical Center Göttingen, 37075, Göttingen, Germany., Sograte-Idrissi S; Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073, Göttingen, Germany.; Center for Biostructural Imaging of Neurodegeneration (BIN), University Medical Center Göttingen, 37075, Göttingen, Germany., Nevskyi O; III. Institute of Physics - Biophysics, Georg August University, 37077, Göttingen, Germany., Gregor I; III. Institute of Physics - Biophysics, Georg August University, 37077, Göttingen, Germany., Opazo F; Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073, Göttingen, Germany.; Center for Biostructural Imaging of Neurodegeneration (BIN), University Medical Center Göttingen, 37075, Göttingen, Germany.; NanoTag Biotechnologies GmbH, 37079, Göttingen, Germany., Thoms S; Department of Child and Adolescent Health, University Medical Center Göttingen, 37073, Göttingen, Germany.; Biochemistry and Molecular Medicine, Medical School, Bielefeld University, 33615, Bielefeld, Germany., Enderlein J; III. Institute of Physics - Biophysics, Georg August University, 37077, Göttingen, Germany. jenderl@gwdg.de.; Cluster of Excellence 'Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells' (MBExC), Georg August University, Göttingen, Germany. jenderl@gwdg.de., Tsukanov R; III. Institute of Physics - Biophysics, Georg August University, 37077, Göttingen, Germany. rtsukan@gwdg.de.

Publikováno v: Communications biology [Commun Biol] 2022 Jan 11; Vol. 5 (1), pp. 38. Date of Electronic Publication: 2022 Jan 11.

Photophysical properties and fluorescence lifetime imaging of exfoliated near-infrared fluorescent silicate nanosheets.

Autor: Selvaggio G; Physical Chemistry II, Bochum University Bochum 44801 Germany Sebastian.Kruss@rub.de.; Institute of Physical Chemistry, University of Göttingen Göttingen 37077 Germany., Weitzel M; Institute of Physical Chemistry, University of Göttingen Göttingen 37077 Germany., Oleksiievets N; Third Institute of Physics, University of Göttingen Göttingen 37077 Germany., Oswald TA; Institute of Organic and Biomolecular Chemistry, University of Göttingen Göttingen 37077 Germany., Nißler R; Physical Chemistry II, Bochum University Bochum 44801 Germany Sebastian.Kruss@rub.de.; Institute of Physical Chemistry, University of Göttingen Göttingen 37077 Germany., Mey I; Institute of Organic and Biomolecular Chemistry, University of Göttingen Göttingen 37077 Germany., Karius V; Department of Sedimentology and Environmental Geology, Geoscience Center, University of Göttingen Göttingen 37077 Germany., Enderlein J; Third Institute of Physics, University of Göttingen Göttingen 37077 Germany.; Cluster of Excellence 'Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells' (MBExC), University of Göttingen Germany., Tsukanov R; Third Institute of Physics, University of Göttingen Göttingen 37077 Germany., Kruss S; Physical Chemistry II, Bochum University Bochum 44801 Germany Sebastian.Kruss@rub.de.; Institute of Physical Chemistry, University of Göttingen Göttingen 37077 Germany.; Fraunhofer Institute for Microelectronic Circuits and Systems Duisburg 47057 Germany.

Publikováno v: Nanoscale advances [Nanoscale Adv] 2021 Jun 24; Vol. 3 (15), pp. 4541-4553. Date of Electronic Publication: 2021 Jun 24 (Print Publication: 2021).