Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Tsuguharu Takahashi"'
Autor:
Tsuguharu Takahashi, Toru Ogasawara, Junji Kishimoto, Guangyao Liu, Hirotaka Asato, Takashi Nakatsuka, Eijyu Uchinuma, Kozo Nakamura, Hiroshi Kawaguchi, Tsuyoshi Takato, Kazuto Hoshi M.D., Ph.D.
Publikováno v:
Cell Transplantation, Vol 14 (2005)
Chondrocyte preparation with the safety and efficiency is the first step in cartilage regenerative medicine. To prepare a chondrocyte proliferation medium that does not contain fetal bovine serum (FBS) and that provides more than a 1000-fold increase
Externí odkaz:
https://doaj.org/article/10d06afd59244d80a4aa35b765c7f79c
Autor:
Tsuguharu Takahashi, Yoshiyuki Mori, Satoru Nishizawa, Tsuyoshi Takato, Ko Matsudaira, Toru Ogasawara, Yukiyo Asawa, Hisayo Yamaoka, Kazuto Hoshi
Publikováno v:
Tissue Engineering Part A. 15:1109-1118
To elucidate the characterizations of chondrocytes originating from auricular cartilage (donors: 10-15 years) and nasoseptal one (20-23 years), we evaluated proliferation or matrix synthesis of both cells cultured under monolayer and collagen type I
Autor:
Hideyuki Suenaga, Shinsuke Ohba, Yuko Fujihara, Toru Ogasawara, Yoshiyuki Mori, Tetsuya Yoda, Takafumi Susami, Kazuto Hoshi, Daichi Chikazu, Ung-il Chung, Guangyao Liu, Tsuyoshi Takato, Tsuguharu Takahashi
Publikováno v:
Asian Journal of Oral and Maxillofacial Surgery. 21:18-26
Objective: To investigate the effects of transforming growth factor-β1 (TGF-β1) in combination with fibroblast growth factor-2 (FGF-2) and insulin-like growth factor-I (IGF-I), on the properties of chondrocytes after proliferation culture. Material
Autor:
Yoshiyuki Mori, Yukiyo Asawa, Tsuyoshi Takato, Kazuto Hoshi, Tsuguharu Takahashi, Toru Ogasawara, Eiju Uchinuma
Publikováno v:
Tissue Engineering. 13:1583-1592
Although autologous chondrocyte implantation has already been in clinical use, chondrocyte dedifferentiation is problematic during proliferation culture. We attempted a three-dimensional (3D) collagen gel culture under chondrocyte proliferation with
Autor:
Toru Ogasawara, Kazuto Hoshi, Tsuyoshi Takato, Eijyu Uchinuma, Junji Kishimoto, Hirotaka Asato, Takashi Nakatsuka, Kozo Nakamura, Tsuguharu Takahashi, Guangyao Liu, Hiroshi Kawaguchi
Publikováno v:
Cell Transplantation, Vol 14 (2005)
Chondrocyte preparation with the safety and efficiency is the first step in cartilage regenerative medicine. To prepare a chondrocyte proliferation medium that does not contain fetal bovine serum (FBS) and that provides more than a 1000-fold increase
Autor:
Koiti Tanaka, Koji Okamoto, Katsumi Suzuki, Masanori Hisaoka, Hideo Ohnishi, Tsuguharu Takahashi, Yousuke Oishi
Publikováno v:
Orthopedics & Traumatology. 45:910-913
Autor:
T Kasama, Yuji Taketani, Yoko Kobayashi, T. Shimizu, Takashi Izumi, F Sato, Takehiko Yokomizo, Tsuguharu Takahashi
Publikováno v:
Journal of Biological Chemistry. 268:18128-18135
Leukotriene B4 (LTB4) 12-hydroxydehydrogenase was purified to apparent homogeneity from the cytosol fraction of the porcine kidney. The N-terminal amino acid sequence analysis revealed that this enzyme is a novel protein with a molecular weight of 35
Autor:
Hirofumi Arakawa, Atsushi Ikai, Yasuko Sakurai, Masaaki Nishigai, T Uchino, Kazunobu Takahashi, Tsuguharu Takahashi
Publikováno v:
Journal of Biological Chemistry. 268:527-533
Porcine stomach mucosa was found to contain a 740-kDa protein having endopeptidase activity toward peptide 4-methylcoumaryl-7-amide substrates and low molecular mass peptides. This protein was purified to an apparent homogeneity by a series of chroma
Autor:
Kozo Nakamura, Yukiyo Asawa, Tsuguharu Takahashi, Hirotaka Asato, Hiroshi Kawaguchi, Jun-ichi Kishimoto, Hisayo Yamaoka, Toru Ogasawara, Tsuyoshi Takato, Guangyao Liu, Ung-il Chung, Kazuto Hoshi
Publikováno v:
The Journal of biological chemistry. 282(28)
Since permanent cartilage has poor self-regenerative capacity, its regeneration from autologous human chondrocytes using a tissue engineering technique may greatly benefit the treatment of various skeletal disorders. However, the conventional autolog
Autor:
Isao Koshima, Tsuguharu Takahashi, Hirotaka Asato, Toru Ogasawara, Kozo Nakamura, Tsuyoshi Takato, Hiroshi Kawaguchi, Satoru Nishizawa, Hisayo Yamaoka, Kazuto Hoshi, Ung-il Chung, Takashi Nakatsuka
Publikováno v:
Journal of biomedical materials research. Part A. 78(1)
To seek a suitable scaffold for cartilage tissue engineering, we compared various hydrogel materials orig- inating from animals, plants, or synthetic peptides. Human auricular chondrocytes were embedded in atelopeptide col- lagen, alginate, or PuraMa