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pro vyhledávání: '"Tsugio Kisou"'
Autor:
Kouichi Kamogawa, Remi Gloeckler, Kazuko Kodama, Denis Speck, Ikuroh Ohsawa, Tsugio Kisou, Ikuhiro Yoneda, Yves Lemoine
Publikováno v:
Journal of Fermentation and Bioengineering. 73:121-124
Bacillus sphaericus was treated with N-acetylmuramidase and transformed with different bioB expression vectors by protoplast transformation, of which only three could be introduced into B. sphaericus. After cultivating the transformants for 24 h at 2