An investigation of the structural requirements for ATP hydrolysis and DNA cleavage by the EcoKI Type I DNA restriction and modification enzyme

Autor: Gareth A. Roberts, Cowan Kennedy, Jakob T. Zipprich, Laurie P. Cooper, David T. F. Dryden, Tsueu-Ju Su, Paul Geary, John H. White

Publikováno v: Roberts, G A, Cooper, L P, White, J H, Su, T-J, Zipprich, J T, Geary, P, Kennedy, C & Dryden, D T F 2011, ' An investigation of the structural requirements for ATP hydrolysis and DNA cleavage by the EcoKI Type I DNA restriction and modification enzyme ', Nucleic Acids Research, vol. 39, no. 17, pp. 7667-7676 . https://doi.org/10.1093/nar/gkr480
Nucleic Acids Research

Type I DNA restriction/modification systems are oligomeric enzymes capable of switching between a methyltransferase function on hemimethylated host DNA and an endonuclease function on unmethylated foreign DNA. They have long been believed to not turn

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a9de3f0377fcb01413786605bf02b76
https://www.pure.ed.ac.uk/ws/files/8735313/An_investigation_of_the_structural_requirements.pdf

Alleviation of restriction by DNA condensation and non-specific DNA binding ligands

Autor: David T. F. Dryden, Steven A. Keatch, Tsueu-Ju Su

Publikováno v: Keatch, S A, Su, T J & Dryden, D T F 2004, ' Alleviation of restriction by DNA condensation and non-specific DNA binding ligands ', Nucleic Acids Research, vol. 32, no. 19, pp. 5841-5850 . https://doi.org/10.1093/nar/gkh918

During conditions of cell stress, the type I restriction and modification enzymes of bacteria show reduced, but not zero, levels of restriction of unmethylated foreign DNA. In such conditions, chemically identical unmethylated recognition sequences a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0adca407be9fd37ee7f466cab51a9b0a
https://pubmed.ncbi.nlm.nih.gov/15520467

Condensation of hydrodynamically stretched DNA using single-molecule fluorescence imaging and optical tweezers

Autor: David T. F. Dryden, Jochen Arlt, Tsueu Ju Su, Wilson C. K. Poon, Eirini Theofanidou, Jason Crain, William J. Hossack

Publikováno v: Optical Trapping and Optical Micromanipulation.

We have observed a latency time of the order 10 s in the spermidine-induced condensation of YOYO-1-labelled DNA in flow. Higher flow speeds, longer DNA and higher salt all led to an increase in the latency time. We propose that two effects may be rel

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b4b35df20814c737669be1f2088f8a6d
https://doi.org/10.1117/12.558896

Interaction of the ocr gene 0.3 protein of bacteriophage T7 with EcoKI restriction/modification enzyme

Autor: C. Atanasiu, David T. F. Dryden, Shane S. Sturrock, Tsueu-Ju Su

Publikováno v: Atanasiu, C, Su, T J, Sturrock, S S & Dryden, D T F 2002, ' Interaction of the ocr gene 0.3 protein of bacteriophage T7 with EcoKI restriction/modification enzyme ', Nucleic Acids Research, vol. 30, no. 18, pp. 3936-3944 . https://doi.org/10.1093/nar/gkf518

The ocr protein, the product of gene 0.3 of bacteriophage T7, is a structural mimic of the phosphate backbone of B-form DNA. In total it mimics 22 phosphate groups over similar to24 bp of DNA. This mimicry allows it to block DNA binding by type I DNA

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17497d0133baf89bdc718d2c6a8eff3c
https://europepmc.org/articles/PMC137103/