Výsledky vyhledávání - "Tsubasa Washio"

The effects of inbreeding depression and pollinator visitation on the maintenance of herkogamy in Oxalis corniculata , a species derived from a heterostylous ancestor

Autor: Yusuke Hoshino, Minori Hoshino, Kazuki Yoshioka, Tsubasa Washio, Makoto Nakamura, Masayuki Maki, Ikumi Dohzono

Publikováno v: Plant Species Biology. 37:349-360

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3c7d6a9dc92be35813b94c7a4844e463
https://doi.org/10.1111/1442-1984.12387

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Thermostable and highly specific l-aspartate oxidase from Thermococcus litoralis DSM 5473: cloning, overexpression, and enzymological properties

Autor: Tadao Oikawa, Tsubasa Washio

Publikováno v: Extremophiles. 22:59-71

We successfully expressed the l-aspartate oxidase homolog gene (accession no: OCC_06611) of Thermococcus litoralis DSM 5473 in the soluble fraction of Escherichia coli BL21 (DE3) using a pET21b vector with 6X His tag at its C-terminus. The gene produ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a578de1f680c7cf05e13c3db39545ee
https://doi.org/10.1007/s00792-017-0977-4

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Molecular cloning and enzymological characterization of pyridoxal 5'-phosphate independent aspartate racemase from hyperthermophilic archaeon Thermococcus litoralis DSM 5473

Autor: Tsubasa Washio, Tadao Oikawa, Shiro Kato

Publikováno v: Extremophiles : life under extreme conditions. 20(5)

We succeeded in expressing the aspartate racemase homolog gene from Thermococcus litoralis DSM 5473 in Escherichia coli Rosetta (DE3) and found that the gene encodes aspartate racemase. The aspartate racemase gene consisted of 687 bp and encoded 228

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1a01e9f9c72262372e6674347d5c2c73
https://pubmed.ncbi.nlm.nih.gov/27438592

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Cloning and characterization of a novel fold-type I branched-chain amino acid aminotransferase from the hyperthermophilic archaeon Thermococcus sp. CKU-1

Autor: Tadao Oikawa, Yuki Uchida, Ryo Yamasaki, Hideyuki Hayashi, Tsubasa Washio, Shiro Kato

Publikováno v: Extremophiles : life under extreme conditions. 18(3)

We successfully cloned a novel branched-chain amino acid aminotransferase (Ts-BcAT; EC 2.6.1.42) gene from the Thermococcus sp. CKU-1 genome and expressed it in the soluble fraction of Escherichia coli Rosetta (DE3) cells. Ts-BcAT is a homodimer with

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68ad656976640f57d0a56c1163fb722b
https://pubmed.ncbi.nlm.nih.gov/24687296

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