The Effect of Detergent, Temperature, and Lipid on the Oligomeric State of MscL Constructs: Insights from Mass Spectrometry

Autor: Idlir Liko, Troy A. Walton, Eamonn Reading, Carol V. Robinson, Arthur Laganowsky, Michael T. Marty, Douglas C. Rees

Publikováno v: Reading, E, Walton, T A, Liko, I, Marty, M T, Laganowsky, A, Rees, D C & Robinson, C V 2015, ' The Effect of Detergent, Temperature, and Lipid on the Oligomeric State of MscL Constructs : Insights from Mass Spectrometry ', Chemistry and Biology, vol. 22, no. 5, pp. 593-603 . https://doi.org/10.1016/j.chembiol.2015.04.016

The mechanosensitive channel of large conductance (MscL) acts as an emergency release valve for osmotic shock of bacteria preventing cell lysis. The large pore size, essential for function, requires the formation of oligomers with tetramers, pentamer

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5692c37a8af1e3448cffdb443ec58653

A reported archaeal mechanosensitive channel is a structural homolog of MarR-like transcriptional regulators

Autor: Troy A. Walton, Douglas C. Rees, Zhenfeng Liu

Publikováno v: Protein Science. 19:808-814

Several archaeal mechanosensitive (MS) channels have been reported, including one from Thermoplasma volcanium designated MscTV. Here, we report the crystal structure of MscTV at 1.6-A resolution. Unexpectedly, MscTV was found to be a water-soluble pr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0eb335c20e8346edb6b355612b62bd44
https://doi.org/10.1002/pro.360

Evaluation of New Linkers and Synthetic Methods for Internal Modified Oligonucleotides

Autor: Ronald M. Cook, Troy A. Walton, Daren J. Dick, Matthew H. Lyttle

Publikováno v: Bioconjugate Chemistry. 13:1155-1158

Several fluorescence resonance energy transfer (FRET) oligonucleotide probes were made with different internal linkages between the DNA and the quencher dye. In one example, a 5'-fluorescein beta-actin-based 26-mer DNA sequence was synthesized bearin

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https://doi.org/10.1021/bc0200125

Structure and stability of the C-terminal helical bundle of the E. coli mechanosensitive channel of large conductance

Autor: Troy A, Walton, Douglas C, Rees

Publikováno v: Protein science : a publication of the Protein Society. 22(11)

The crystal structure of the cytoplasmic domain (CTD) from the mechanosensitive channel of large conductance (MscL) in E. coli has been determined at 1.45 Å resolution. This domain forms a pentameric coiled coil similar to that observed in the struc

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https://pubmed.ncbi.nlm.nih.gov/24038743

A reported archaeal mechanosensitive channel is a structural homolog of MarR-like transcriptional regulators

Autor: Zhenfeng, Liu, Troy A, Walton, Douglas C, Rees

Publikováno v: Protein science : a publication of the Protein Society. 19(4)

Several archaeal mechanosensitive (MS) channels have been reported, including one from Thermoplasma volcanium designated MscTV. Here, we report the crystal structure of MscTV at 1.6-Å resolution. Unexpectedly, MscTV was found to be a water-soluble p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::3ef36a71e3f6162cbab095255df0c0a8
https://pubmed.ncbi.nlm.nih.gov/20162616