Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Tricia A. Harvat"'
Autor:
Daniel H. Paushter, Meirigeng Qi, Kirstie K. Danielson, Tricia A. Harvat, Katie Kinzer, Barbara Barbaro, Sonny Patel, Sarah Z. Hassan, Jose Oberholzer, Yong Wang
Publikováno v:
Cell Transplantation, Vol 22 (2013)
We previously reported a small-scale study on the efficacy of histidine-tryptophan-ketoglutarate (HTK) solution versus University of Wisconsin (UW) solution on pancreas preservation for islet isolation. In this large-scale, retrospective analysis ( n
Externí odkaz:
https://doaj.org/article/bbf422f6ffd4453cbb722721bddb5eb9
Autor:
Yong Wang, Joshua E. Mendoza-Elias, Meirigeng Qi, Tricia A. Harvat, Sang Joon Ahn, Dongyoung Lee, Diana Gutierrez, Hyojin Jeon, Daniel Paushter, José Oberholzer
Publikováno v:
Biochemistry Research International, Vol 2012 (2012)
Islet transplantation is a promising therapy for type 1 diabetes mellitus; however, success rates in achieving both short- and long-term insulin independence are not consistent, due in part to inconsistent islet quality and quantity caused by the com
Externí odkaz:
https://doaj.org/article/cbed408980234a90b4c0d275ca3704d9
Publikováno v:
Journal of the American College of Surgeons. 233:e166
Autor:
Hyojin Jeon, Yong Wang, Sarah Z. Hassan, Jose Oberholzer, Joshua E. Mendoza-Elias, Amanda Zhou, Lisa Zhang, Tricia A. Harvat, Dongyoung Lee, David T. Eddington
Publikováno v:
Biomedical Microdevices. 14:419-426
Reliable long-term cell culture in microfluidic system is limited by air bubble formation and accumulation. In this study, we developed a bubble removal system capable of both trapping and discharging air bubbles in a consistent and reliable manner.
Autor:
Adeola F. Adewola, Jose Oberholzer, Meirigeng Qi, Diana Gutierrez, Katie Kinzer, Joshua E. Mendoza-Elias, Yong Wang, Tricia A. Harvat, David T. Eddington, Dongyoung Lee
Publikováno v:
Biomedical Microdevices. 14:7-16
In this study, a new class of duplex microfluidic device is described that utilizes a dual perifusion network to simultaneously perform live-cell optical imaging of physiological activities and study insulin release kinetics on two islet populations.
Autor:
Dongyoung Lee, Jose Oberholzer, David T. Eddington, Joe F. Lo, Meirigeng Qi, Adeola F. Adewola, Yong Wang, Tricia A. Harvat, Katie Kinzer, Joshua E. Mendoza-Elias
Publikováno v:
Bioanalysis. 2:1729-1744
β-cells respond to blood glucose by secreting insulin to maintain glucose homeostasis. Perifusion enables manipulation of biological and chemical cues in elucidating the mechanisms of β-cell physiology. Recently, microfluidic devices made of polydi
Autor:
Adeola F. Adewola, Dongyoung Lee, Shusen Wang, Jose Oberholzer, Tricia A. Harvat, Enrico Benedetti, Rafael A. Vega, Yong Wang, Merigeng Qi
Publikováno v:
Surgery. 148:858-866
Background Islet transplantation is an emerging therapy for type 1 diabetes mellitus with variable success. Molecular therapeutics is a promising approach to improve islet graft function and transplant outcomes. Traditional delivery vectors, however,
Autor:
Yong Wang, David T. Eddington, Javeed Shaikh Mohammed, Jose Oberholzer, Tricia A. Harvat, Dongyoung Lee, Adeola F. Adewola
Publikováno v:
Biomedical Microdevices. 12:409-417
A microfluidic islet perifusion device was developed for the assessment of dynamic insulin secretion of multiple pancreatic islets and simultaneous fluorescence imaging of calcium influx and mitochondrial potential changes. The fanned out design of t
Autor:
Yong Wang, Feng Feng, Katie Kinzer, Shusen Wang, Tricia A. Harvat, Jose Oberholzer, Meirigeng Qi, Lisa Zhang
Publikováno v:
Cell Transplantation, Vol 24 (2015)
Diazoxide (DZ) is a pharmacological opener of ATP-sensitive K+ channels that has been used for mimicking ischemic preconditioning and shows protection against ischemic damage. Here we investigated whether diazoxide supplementation to University of Wi
Autor:
Meirigeng Qi, Kirstie K. Danielson, Yong Wang, Jose Oberholzer, A. Ropski, Tricia A. Harvat, Barbara Barbaro, Daniel H. Paushter
Publikováno v:
Cell Transplantation, Vol 22 (2013)
Islet transplantation is a promising therapy for type I diabetes mellitus, with both islet yield and islet size playing important roles in transplant outcomes. Some key factors influencing islet yield have been identified, but with conflicting result