Protocol for engineering binding domains to recognize ligand-bound receptors by using yeast surface display.

Autor: Dobersberger M; Department of Chemistry, Institute of Biochemistry, BOKU University, Vienna 1190, Austria., Sumesgutner D; Department of Chemistry, Institute of Biochemistry, BOKU University, Vienna 1190, Austria; CD Laboratory for Next Generation CAR T Cells, Vienna 1090, Austria. Electronic address: delia.sumesgutner@boku.ac.at., Zajc CU; Department of Chemistry, Institute of Biochemistry, BOKU University, Vienna 1190, Austria; CD Laboratory for Next Generation CAR T Cells, Vienna 1090, Austria., Traxlmayr MW; Department of Chemistry, Institute of Biochemistry, BOKU University, Vienna 1190, Austria; CD Laboratory for Next Generation CAR T Cells, Vienna 1090, Austria. Electronic address: michael.traxlmayr@boku.ac.at.

Publikováno v: STAR protocols [STAR Protoc] 2024 Sep 24; Vol. 5 (4), pp. 103339. Date of Electronic Publication: 2024 Sep 24.

An engineering strategy to target activated EGFR with CAR T cells.

Autor: Dobersberger M; Department of Chemistry, Institute of Biochemistry, BOKU University, 1190 Vienna, Austria., Sumesgutner D; Department of Chemistry, Institute of Biochemistry, BOKU University, 1190 Vienna, Austria; CD Laboratory for Next Generation CAR T Cells, 1090 Vienna, Austria., Zajc CU; Department of Chemistry, Institute of Biochemistry, BOKU University, 1190 Vienna, Austria; CD Laboratory for Next Generation CAR T Cells, 1090 Vienna, Austria., Salzer B; CD Laboratory for Next Generation CAR T Cells, 1090 Vienna, Austria; St. Anna Children's Cancer Research Institute, CCRI, 1090 Vienna, Austria., Laurent E; BOKU Core Facility Biomolecular & Cellular Analysis, BOKU University, 1190 Vienna, Austria., Emminger D; CD Laboratory for Next Generation CAR T Cells, 1090 Vienna, Austria; St. Anna Children's Cancer Research Institute, CCRI, 1090 Vienna, Austria., Sylvander E; CD Laboratory for Next Generation CAR T Cells, 1090 Vienna, Austria; St. Anna Children's Cancer Research Institute, CCRI, 1090 Vienna, Austria., Lehner E; Department of Chemistry, Institute of Biochemistry, BOKU University, 1190 Vienna, Austria; CD Laboratory for Next Generation CAR T Cells, 1090 Vienna, Austria., Teufl M; Department of Chemistry, Institute of Biochemistry, BOKU University, 1190 Vienna, Austria; CD Laboratory for Next Generation CAR T Cells, 1090 Vienna, Austria., Seigner J; Department of Chemistry, Institute of Biochemistry, BOKU University, 1190 Vienna, Austria; Department of Biotechnology, Institute of Animal Cell Technology and Systems Biology, BOKU University, 1190 Vienna, Austria., Bobbili MR; Department of Biotechnology, Institute of Molecular Biotechnology, BOKU University, 1190 Vienna, Austria; Ludwig Boltzmann Institute for Traumatology, Research Center in Cooperation with AUVA, 1200 Vienna, Austria., Kunert R; Department of Biotechnology, Institute of Animal Cell Technology and Systems Biology, BOKU University, 1190 Vienna, Austria., Lehner M; CD Laboratory for Next Generation CAR T Cells, 1090 Vienna, Austria; St. Anna Children's Cancer Research Institute, CCRI, 1090 Vienna, Austria; St. Anna Children's Hospital, Department of Pediatrics, Medical University of Vienna, 1090 Vienna, Austria., Traxlmayr MW; Department of Chemistry, Institute of Biochemistry, BOKU University, 1190 Vienna, Austria; CD Laboratory for Next Generation CAR T Cells, 1090 Vienna, Austria. Electronic address: michael.traxlmayr@boku.ac.at.

Publikováno v: Cell reports methods [Cell Rep Methods] 2024 Apr 22; Vol. 4 (4), pp. 100728. Date of Electronic Publication: 2024 Mar 15.

Identification of Activating Mutations in the Transmembrane and Extracellular Domains of EGFR.

Autor: Wagner A; Department of Chemistry, Institute of Biochemistry, University of Natural Resources and Life Sciences, 1190 Vienna, Austria.; Department of Biotechnology, Institute of Molecular Biotechnology, University of Natural Resources and Life Sciences, 1190 Vienna, Austria., Galicia-Andrés E; Department of Material Sciences and Process Engineering, Institute of Molecular Modeling and Simulation, University of Natural Resources and Life Sciences, 1190 Vienna, Austria.; Department of Forest and Soil Sciences, Institute of Soil Research, University of Natural Resources and Life Sciences, 1190 Vienna, Austria., Teufl M; Department of Chemistry, Institute of Biochemistry, University of Natural Resources and Life Sciences, 1190 Vienna, Austria., Gold L; Department of Chemistry, Institute of Biochemistry, University of Natural Resources and Life Sciences, 1190 Vienna, Austria., Obinger C; Department of Chemistry, Institute of Biochemistry, University of Natural Resources and Life Sciences, 1190 Vienna, Austria., Sykacek P; Department of Biotechnology, Institute for Computational Biology, University of Natural Resources and Life Sciences, 1190 Vienna, Austria., Oostenbrink C; Department of Material Sciences and Process Engineering, Institute of Molecular Modeling and Simulation, University of Natural Resources and Life Sciences, 1190 Vienna, Austria., Traxlmayr MW; Department of Chemistry, Institute of Biochemistry, University of Natural Resources and Life Sciences, 1190 Vienna, Austria.

Publikováno v: Biochemistry [Biochemistry] 2022 Oct 04; Vol. 61 (19), pp. 2049-2062. Date of Electronic Publication: 2022 Sep 22.

Affinity and Stability Analysis of Yeast Displayed Proteins.

Autor: Zajc CU; Department of Chemistry, Institute of Biochemistry, University of Natural Resources and Life Sciences, Vienna, Austria.; CD Laboratory for Next Generation CAR T Cells, Vienna, Austria., Teufl M; Department of Chemistry, Institute of Biochemistry, University of Natural Resources and Life Sciences, Vienna, Austria.; CD Laboratory for Next Generation CAR T Cells, Vienna, Austria., Traxlmayr MW; Department of Chemistry, Institute of Biochemistry, University of Natural Resources and Life Sciences, Vienna, Austria. michael.traxlmayr@boku.ac.at.

Publikováno v: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2491, pp. 155-173.

Peroxidasin protein expression and enzymatic activity in metastatic melanoma cell lines are associated with invasive potential.

Autor: Paumann-Page M; Centre for Free Radical Research, Department of Pathology and Biomedical Science, University of Otago Christchurch, P.O. Box 4345, Christchurch, New Zealand. Electronic address: martina.paumann-page@otago.ac.nz., Kienzl NF; Department of Chemistry, Institute of Biochemistry, University of Natural Resources and Life Sciences, 1190, Vienna, Austria., Motwani J; Department of Pathology, Dunedin School of Medicine, University of Otago, 270 Great King Street, Dunedin, 9054, New Zealand., Bathish B; Centre for Free Radical Research, Department of Pathology and Biomedical Science, University of Otago Christchurch, P.O. Box 4345, Christchurch, New Zealand., Paton LN; Centre for Free Radical Research, Department of Pathology and Biomedical Science, University of Otago Christchurch, P.O. Box 4345, Christchurch, New Zealand., Magon NJ; Centre for Free Radical Research, Department of Pathology and Biomedical Science, University of Otago Christchurch, P.O. Box 4345, Christchurch, New Zealand., Sevcnikar B; Department of Chemistry, Institute of Biochemistry, University of Natural Resources and Life Sciences, 1190, Vienna, Austria., Furtmüller PG; Department of Chemistry, Institute of Biochemistry, University of Natural Resources and Life Sciences, 1190, Vienna, Austria., Traxlmayr MW; Department of Chemistry, Institute of Biochemistry, University of Natural Resources and Life Sciences, 1190, Vienna, Austria., Obinger C; Department of Chemistry, Institute of Biochemistry, University of Natural Resources and Life Sciences, 1190, Vienna, Austria., Eccles MR; Department of Pathology, Dunedin School of Medicine, University of Otago, 270 Great King Street, Dunedin, 9054, New Zealand., Winterbourn CC; Centre for Free Radical Research, Department of Pathology and Biomedical Science, University of Otago Christchurch, P.O. Box 4345, Christchurch, New Zealand.

Publikováno v: Redox biology [Redox Biol] 2021 Oct; Vol. 46, pp. 102090. Date of Electronic Publication: 2021 Aug 04.