Zobrazeno 1 - 10
of 1 291
pro vyhledávání: '"Transient transfection"'
Autor:
Pol Pérez-Rubio, Meritxell Vendrell-Flotats, Elianet Lorenzo Romero, Kasper Enemark-Rasmussen, Laura Cervera, Francesc Gòdia, Jesús Lavado-García
Publikováno v:
Biomedicine & Pharmacotherapy, Vol 176, Iss , Pp 116893- (2024)
Polymer-cationic mediated gene delivery is a well-stablished strategy of transient gene expression (TGE) in mammalian cell cultures. Nonetheless, its industrial implementation is hindered by the phenomenon known as cell density effect (CDE) that limi
Externí odkaz:
https://doaj.org/article/e77ee55ac09d45a6aeded7d245d74ce9
Autor:
Thomas Williams-Fegredo, Lee Davies, Carol Knevelman, Kyriacos Mitrophanous, James Miskin, Qasim A. Rafiq
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 12 (2024)
Both cell surface and soluble extracellular glycosaminoglycans have been shown to interfere with the exogenous nucleic acid delivery efficiency of non-viral gene delivery, including lipoplex and polyplex-mediated transfection. Most gene therapy viral
Externí odkaz:
https://doaj.org/article/30f18ce1f17f438ab64d69f10643272c
Autor:
Thomas Williams-Fegredo, Lee Davies, Carol Knevelman, Kyriacos Mitrophanous, James Miskin, Qasim A. Rafiq
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 32, Iss 2, Pp 101260- (2024)
Large-scale transient transfection has advanced significantly over the last 20 years, enabling the effective production of a diverse range of biopharmaceutical products, including viral vectors. However, a number of challenges specifically related to
Externí odkaz:
https://doaj.org/article/3197910bb8934f0bb12e559c15fef757
Autor:
Yizong Hu, Brendan A. Eder, Jinghan Lin, Sixuan Li, Yining Zhu, Tza-Huei Wang, Ting Guo, Hai-Quan Mao
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 32, Iss 1, Pp 101194- (2024)
The transfection efficiency and stability of the delivery vehicles of plasmid DNA (pDNA) are critical metrics to ensure high-quality and high-yield production of viral vectors. We previously identified that the optimal size of pDNA/poly(ethylenimine)
Externí odkaz:
https://doaj.org/article/15de10c460aa4bb3a0fa8b2a57e0f9f7
Autor:
Wenhui Yang, Jiaqin Ren, Wanrong Liu, Dan Liu, Kaidong Xie, Fei Zhang, Pengwei Wang, Wenwu Guo, Xiaomeng Wu
Publikováno v:
Horticultural Plant Journal, Vol 9, Iss 3, Pp 425-436 (2023)
Protoplast has been widely used in biotechnologies to circumvent the breeding obstacles in citrus, including long juvenility, polyembryony, and male/female sterility. The protoplast-based transient gene expression system is a powerful tool for gene f
Externí odkaz:
https://doaj.org/article/b9909edd8fd5453d8b5b2c6936e6ce9d
Autor:
Laura P. van Lieshout, Miranda Rubin, Katrina Costa-Grant, Stacy Ota, Diane Golebiowski, Troy Panico, Eli Wiberg, Klaudia Szymczak, Richard Gilmore, Marissa Stanvick, Brenda Burnham, Jeff Gagnon, Ifeyinwa Iwuchukwu, Guang Yang, Iraj Ghazi, Alex Meola, Ryan Dickerson, Thomas Thiers, Luke Mustich, April Hayes, Israel Rivas, Jason Lotterhand, Nancy Avila, James McGivney, Jin Yin, Tim Kelly
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 29, Iss , Pp 426-436 (2023)
Transient transfection of mammalian cells using plasmid DNA is a standard method to produce adeno-associated virus (AAV) vectors allowing for flexible and scalable manufacture. Typically, three plasmids are used to encode the necessary components to
Externí odkaz:
https://doaj.org/article/25fd1b8281394ec9b15853de27025cb5
Autor:
Vaneet K. Sharma, Sergey Menis, Evan T. Brower, Eddy Sayeed, Jim Ackland, Angela Lombardo, Christopher A. Cottrell, Jonathan L. Torres, Thomas Hassell, Andrew B. Ward, Vadim Tsvetnitsky, William R. Schief
Publikováno v:
Pharmaceutics, Vol 16, Iss 6, p 742 (2024)
We describe the current Good Manufacturing Practice (cGMP) production and subsequent characterization of eOD-GT8 60mer, a glycosylated self-assembling nanoparticle HIV-1 vaccine candidate and germline targeting priming immunogen. Production was carri
Externí odkaz:
https://doaj.org/article/a5c660dbcce146b78053fb5ed221bb13
Publikováno v:
International Journal of Nanomedicine, Vol Volume 17, Pp 4469-4479 (2022)
Kathryn M Luly,1,2 Huilin Yang,2,3 Stephen J Lee,2,3 Wentao Wang,1,2 Seth D Ludwig,2,3 Haley E Tarbox,4 David R Wilson,1,2,5 Jordan J Green,1– 3,5– 10 Jamie B Spangler1– 3,6,8 1Department of Biomedical Engineering, Johns Hopkins University Scho
Externí odkaz:
https://doaj.org/article/8630b56c0d8344299d0604b9574d0f19
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