EspH is a hypervirulence factor for Mycobacterium marinum and essential for the secretion of the ESX-1 substrates EspE and EspF.

Autor: Trang H Phan, Lisanne M van Leeuwen, Coen Kuijl, Roy Ummels, Gunny van Stempvoort, Alba Rubio-Canalejas, Sander R Piersma, Connie R Jiménez, Astrid M van der Sar, Edith N G Houben, Wilbert Bitter

Publikováno v: PLoS Pathogens, Vol 14, Iss 8, p e1007247 (2018)

The pathogen Mycobacterium tuberculosis employs a range of ESX-1 substrates to manipulate the host and build a successful infection. Although the importance of ESX-1 secretion in virulence is well established, the characterization of its individual c

Externí odkaz: https://doaj.org/article/168aa6b95d0e4e7c9b73b8e47bd923a8

Overproducing the BAM complex improves secretion of difficult-to-secrete recombinant autotransporter chimeras

Autor: Coen Kuijl, Peter van Ulsen, Trang H Phan, Joen Luirink, Dung T Huynh, Wouter S. P. Jong

Publikováno v: Microbial Cell Factories, 20(1):176. BioMed Central
Microbial Cell Factories
Microbial Cell Factories, Vol 20, Iss 1, Pp 1-17 (2021)
Phan, T H, Kuijl, C, Huynh, D T, Jong, W S P, Luirink, J & van Ulsen, P 2021, ' Overproducing the BAM complex improves secretion of difficult-to-secrete recombinant autotransporter chimeras ', Microbial Cell Factories, vol. 20, no. 1, 176 . https://doi.org/10.1186/s12934-021-01668-2
Microbial Cell Factories, 20(1):176. BioMed Central Ltd.
Phan, T H, Kuijl, CP, Huynh, D, Jong, W S P, Luirink, J & van Ulsen, JP 2021, ' Overproducing the BAM complex improves secretion of difficult-to-secrete recombinant autotransporter chimeras ', Microbial Cell Factories, vol. 20, no. 1, 176 . https://doi.org/10.1186/s12934-021-01668-2

Monomeric autotransporters have been used extensively to transport recombinant proteins or protein domains to the cell surface of Gram-negative bacteria amongst others for antigen display. Genetic fusion of such antigens into autotransporters has yie

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb5eb0d601d0c9fa89200956987e856a
https://research.vu.nl/en/publications/72599a24-d465-4485-a0f9-62848b52708a

Rerouting of an Esx substrate pair from the ESX-1 type VII secretion system to ESX-5 by modifying a PE/PPE substrate pair

Autor: Merel P.M. Damen, Trang H Phan, Edith N.G. Houben, Alba Rubio-Canalejas, Roy Ummels, Wilbert Bitter

Type VII secretion systems (T7SSs) secrete a wide range of extracellular proteins that play important roles in bacterial viability and in host-pathogen interactions of pathogenic mycobacteria. There are five subtypes of mycobacterial T7SSs, called ES

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f1a5964dbcbf1e0595e1e9b6bbf9d55c

EspH is a hypervirulence factor for Mycobacterium marinum and essential for the secretion of the ESX-1 substrates EspE and EspF

Autor: Lisanne M van Leeuwen, Roy Ummels, Sander R. Piersma, Trang H Phan, Coen Kuijl, Gunny van Stempvoort, Alba Rubio-Canalejas, Connie R. Jimenez, Edith N.G. Houben, Astrid M. van der Sar, Wilbert Bitter

Publikováno v: Phan, T H, van Leeuwen, L M, Kuijl, C, Ummels, R, van Stempvoort, G, Rubio-Canalejas, A, Piersma, S R, Jiménez, C R, van der Sar, A M, Houben, E N G & Bitter, W 2018, ' EspH is a hypervirulence factor for Mycobacterium marinum and essential for the secretion of the ESX-1 substrates EspE and EspF ', PLoS Pathogens, vol. 14, no. 8, pp. e1007247 . https://doi.org/10.1371/journal.ppat.1007247
PLoS Pathogens, 14(8). Public Library of Science
PLoS Pathogens, Vol 14, Iss 8, p e1007247 (2018)
Phan, T H, van Leeuwen, L M, Kuijl, C, Ummels, R, van Stempvoort, G, Rubio-Canalejas, A, Piersma, S R, Jiménez, C R, van der Sar, A M, Houben, E N G & Bitter, W 2018, ' EspH is a hypervirulence factor for Mycobacterium marinum and essential for the secretion of the ESX-1 substrates EspE and EspF ', Plos Pathogens, vol. 14, no. 8, e1007247, pp. 1-26 . https://doi.org/10.1371/journal.ppat.1007247
Plos Pathogens, 14(8):e1007247, 1-26. Public Library of Science
PLoS Pathogens

The pathogen Mycobacterium tuberculosis employs a range of ESX-1 substrates to manipulate the host and build a successful infection. Although the importance of ESX-1 secretion in virulence is well established, the characterization of its individual c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8fd7b8d7fc3307c387fb76b5576a30ad
https://doi.org/10.1371/journal.ppat.1007247

Structure of theMycobacterium tuberculosistype VII secretion system chaperone EspG5in complex with PE25-PPE41 dimer

Autor: Roy Ummels, Christopher C. Creekmore, Annabel H. A. Parret, Trang H. Phan, Wilbert Bitter, Matthias Wilmanns, Diana Freire, Timothy J. Evans, Edith N.G. Houben, Konstantin V. Korotkov, Natalia Korotkova

Publikováno v: Molecular Microbiology. 94:367-382

The growth or virulence of Mycobacterium tuberculosis bacilli depends on homologous type VII secretion systems, ESX-1, ESX-3 and ESX-5, which export a number of protein effectors across membranes to the bacterial surface and environment. PE and PPE p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::59cca80a0530ff9d94eb47ee235a4e80
https://doi.org/10.1111/mmi.12770