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Autor:
Meir Fischer, Ilana Liefer, Marian Gorecki, Ruth Lifshitz, Elisha Zeelon, Amos Panet, Tova Katz, Hana Ben-Artzi
Publikováno v:
Protein expression and purification. 3(4)
To study the subunit structure and the active site of human immunodeficiency virus reverse transcriptase (RT), the enzyme was expressed in E. coli and purified to homogeneity in large quantities. The recombinant enzyme consists of two major polypepti