Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Touho Yoshida"'
Autor:
Kazumi Ono, Yutaka Ozawa, Masao Takeuchi, Motonobu Satoh, Hiroshi Mizusawa, Hidekazu Sawada, Touho Yoshida, Arihiro Kohara
Publikováno v:
In Vitro Cellular & Developmental Biology - Animal. 43:168-175
We developed a highly sensitive and convenient method of nested polymerase chain reaction (PCR) targeted to mitochondrial deoxyribonucleic acid (DNA) to identify animal species quickly in cultured cells. Fourteen vertebrate species, including human,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::452b3e3acccb2da5b68356a0098637a4
https://doi.org/10.1007/s11626-007-9033-5
https://doi.org/10.1007/s11626-007-9033-5
Publikováno v:
Cytotechnology. 29:221-227
Reverse transcriptase (RT) is a good diagnostic tool for the detection of retroviruses. We have developed a simple and rapid assay for RT activity in culture supernatants. A 370-base RNA sequence from the tetracycline-resistance gene in pBR322 plasmi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2c2c9482a57af6344868ca6e07133292
https://doi.org/10.1023/a:1008000210125
https://doi.org/10.1023/a:1008000210125
Autor:
Motonobu Satoh, Akira Hakura, Masao Takeuchi, Toshi Iwama, Haruhiko Kuno, Yumiko Nakagaito, Touho Yoshida
Publikováno v:
In Vitro Cellular & Developmental Biology - Animal. 34:585-592
We have established a multipotent clonal cell line, named MEB5, from embryonic mouse forebrains after the infection of a retrovirus carrying E7 oncogene of human papillomavirus type 16. MEB5 cells proliferated in serum-free, epidermal growth factor (
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3262bd1135def9dedbce2488d23a2d0
https://doi.org/10.1007/s11626-998-0119-5
https://doi.org/10.1007/s11626-998-0119-5
Publikováno v:
Developmental Brain Research. 87:220-223
The effects of leukemia inhibitory factor (LIF) on the differentiation and proliferation of astrocyte progenitor cells in an enriched culture were studied. The astrocyte progenitor cells obtained from embryonic mouse cerebral hemispheres were enriche
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a194fc2ebc26aadb4a1ea1cc43f0b21
https://doi.org/10.1016/0165-3806(95)00063-j
https://doi.org/10.1016/0165-3806(95)00063-j
Publikováno v:
Developmental Brain Research. 76:147-150
The effects of various cytokines on survival and differentiation of an astrocyte progenitor cell line (AP-16) were examined. Epidermal growth factor (EGF) deprivation caused death of AP-16 cells by apoptosis. Transforming growth factor-alpha (TGF-alp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29c56eddf842580fa50f6aa8d0b4e78f
https://doi.org/10.1016/0165-3806(93)90132-t
https://doi.org/10.1016/0165-3806(93)90132-t
Autor:
Touho Yoshida
Publikováno v:
Nihon yakurigaku zasshi. Folia pharmacologica Japonica. 134(6)
ヒューマンサイエンス研究資源バンクは，厚生労働省支援のもとに1995年に開設され，（独）医薬基盤研究所で収集，品質管理した細胞，遺伝子を産学官の研究者に有償で分譲している．
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d41229bb901eb961c3c71a47a1548405
https://pubmed.ncbi.nlm.nih.gov/20009364
https://pubmed.ncbi.nlm.nih.gov/20009364
Autor:
Touho Yoshida, Masao Takeuchi
Publikováno v:
Cytotechnology. 7:187-196
The expression of fibronectin and laminin by cultured glial cells was studied. The glial culture from neonatal mouse cerebra maintained in a chemically defined, serum-free medium consisted of type-1 astrocytes, oligodendrocyte-type-2 astrocyte (O-2A)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72ad6e8ba496ce2df40aa3f85f04cdd4
https://doi.org/10.1007/bf00365930
https://doi.org/10.1007/bf00365930
Autor:
Masao Takeuchi, Touho Yoshida
Publikováno v:
Cytotechnology. 5:99-106
The methods of primary culture and cryopreservation of mouse astrocytes under serum-free conditions were examined. Cerebra from newborn C3H/He mice were employed as the source of astrocytes. The cultured cells were able to grow in a serum-free, chemi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::37847d18f9d851da6503fbe2775e1b5b
https://doi.org/10.1007/bf00365426
https://doi.org/10.1007/bf00365426
Autor:
Touho Yoshida, Motonobu Satoh
Publikováno v:
Brain research. Developmental brain research. 121(2)
A cytokeratin (CK)(+), horizontal basal cell (HBC)-like cell line from murine olfactory epithelium, DBC1.2, was characterized. Histochemically, it is CK14(+), CK18(-), neuron-specific tubulin (NST)(+) and capable of binding Bandeiraea simplicifolia l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::69c662cb149b389b2855700655391eef
https://pubmed.ncbi.nlm.nih.gov/10876035
https://pubmed.ncbi.nlm.nih.gov/10876035
Publikováno v:
Neuroscience letters. 284(3)
The effects of activin A were investigated on the development of a multipotent neural stem cell line (MEB5) and an astrocyte progenitor cell line (AP-16) that were established from murine central nervous system (CNS). Reverse transcriptase-polymerase
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91eceb1a0de1a05bf6f5365334f803f5
https://pubmed.ncbi.nlm.nih.gov/10773419
https://pubmed.ncbi.nlm.nih.gov/10773419