Zobrazeno 1 - 10
of 40
pro vyhledávání: '"Toshitaka Akisaka"'
Autor:
Toshitaka Akisaka
Publikováno v:
Journal of Oral Biosciences. 63:307-318
Background Preserving the cellular structure at the highest possible resolution is a prerequisite for morphological studies to deepen our understanding of cellular functions. A revival of interest in rapid-freezing methods combined with breaking-open
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bfb5a82c9dc5fbb51e7c39b08300087b
https://doi.org/10.1016/j.job.2021.09.006
https://doi.org/10.1016/j.job.2021.09.006
Autor:
Toshitaka Akisaka, Atsushi Yoshida
Publikováno v:
Experimental Cell Research. 399:112433
Osteoclasts seeded on either glass coverslips or apatite pellets have at least two morphologically distinct substrate adhesion sites: actin-based adhesion structures including podosome belts and sealing zones, and adjacent clathrin sheets. Clathrin-c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30e12ee7e4327c24eeb31650ac6a230f
https://doi.org/10.1016/j.yexcr.2020.112433
https://doi.org/10.1016/j.yexcr.2020.112433
Autor:
Toshitaka Akisaka, Atsushi Yoshida
Publikováno v:
Cytoskeleton (Hoboken, N.J.). 76(4)
Osteoclasts (OCs) can adhere to a variety of substrate surfaces by highly dynamic actin-based cytoskeletal structures termed podosomes. This tight attachment is established by a sealing zone (SZ), which is made of interconnected individual podosomes.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aadafd838e748dcbdab16b419981bcbb
https://pubmed.ncbi.nlm.nih.gov/31162808
https://pubmed.ncbi.nlm.nih.gov/31162808
Autor:
Toshitaka Akisaka, Astushi Yoshida
Publikováno v:
Micron (Oxford, England : 1993). 88
Osteoclasts in culture are non-transformed cell types that spontaneously develop specific cell-adhesion devices such as podosomes. An individual podosome is a complex network of filamentous actin (F-actin) unit structure that collectively, with other
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63940dc387bc1c2116df6d568e1f3d23
https://pubmed.ncbi.nlm.nih.gov/27323283
https://pubmed.ncbi.nlm.nih.gov/27323283
Publikováno v:
Journal of Bone and Mineral Research. 3:19-25
The ultrastructural distribution of Ca++-ATPase in bone cells of growing chick tibia was investigated by a cytochemical method in order to gain insight into possible sites of calcium ion translocation. Both osteoclasts and osteoblasts showed a polar
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f48953d22b7ed3de8644c5472bc42cb
https://doi.org/10.1002/jbmr.5650030105
https://doi.org/10.1002/jbmr.5650030105
Publikováno v:
Journal of Electron Microscopy. 55:53-61
The aim of our present research was to visualize how the plasma membrane is modified and how the cytoskeleton interacts with the attachment and ruffled border regions of resorbing osteoclasts. In order to view the surface modification of membranes an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d466446917e0dadd334c723ec847b156
https://doi.org/10.1093/jmicro/dfl012
https://doi.org/10.1093/jmicro/dfl012
Publikováno v:
Journal of Electron Microscopy. 52:535-543
Physical cell-shearing resulted in various degrees of disruption of the basolateral (upper) membranes, cytoskeletons or cell organelles and exposed the protoplasmic surface of ventral (adhesion) membranes of osteoclasts that were attached to the unde
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11655a9e0b51c13fe6fa60339b7c7a53
https://doi.org/10.1093/jmicro/52.6.535
https://doi.org/10.1093/jmicro/52.6.535
Publikováno v:
Tissue and Cell. 35:189-197
Osteocytes are released from the osteocytic lacunae when osteoclasts resorb the bone matrix during bone modeling and remodeling. It remains unknown how osteoclasts react when releasing osteocytes during bone modeling, and the fate of these released o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::682f3defcb676c09f487c82ddf85ba0b
https://doi.org/10.1016/s0040-8166(03)00020-x
https://doi.org/10.1016/s0040-8166(03)00020-x
Autor:
Toshitaka Akisaka, Atsushi Yoshida
Publikováno v:
Cell and tissue research. 360(2)
Osteoclasts are highly polarized cells from both morphological and functional points of view. Using quick-freeze, rotary-replication methods combined with cell-shearing, we clarified the variability of cytoplasmic surface of the polarized membranes o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::141d797b058fdc011c9bc2173cc8008c
https://pubmed.ncbi.nlm.nih.gov/25582780
https://pubmed.ncbi.nlm.nih.gov/25582780
Publikováno v:
European Journal of Protistology. 36:303-315
Summary For better preservation of Amoeba proteus ultrastructure, we have applied the quick-freezing and freeze-substitution method to them which are difficult to fix optimally for conventional electron microscopy. This method provided a greatly impr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2da6cc48c7e395af9f7d4a68c9184d64
https://doi.org/10.1016/s0932-4739(00)80006-1
https://doi.org/10.1016/s0932-4739(00)80006-1