Zobrazeno 1 - 10
of 145
pro vyhledávání: '"Toshifumi Inada"'
Autor:
Ken Ikeuchi, Nives Ivic, Robert Buschauer, Jingdong Cheng, Thomas Fröhlich, Yoshitaka Matsuo, Otto Berninghausen, Toshifumi Inada, Thomas Becker, Roland Beckmann
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-15 (2023)
Abstract In actively translating 80S ribosomes the ribosomal protein eS7 of the 40S subunit is monoubiquitinated by the E3 ligase Not4 and deubiquitinated by Otu2 upon ribosomal subunit recycling. Despite its importance for translation efficiency the
Externí odkaz:
https://doaj.org/article/399c964c1fa34db4b2366a5130139ce8
Autor:
Katharina Best, Ken Ikeuchi, Lukas Kater, Daniel Best, Joanna Musial, Yoshitaka Matsuo, Otto Berninghausen, Thomas Becker, Toshifumi Inada, Roland Beckmann
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-12 (2023)
Ribosome collisions serve as proxy for aberrant translation to initiate rescue and quality control pathways. Here, authors elucidate the molecular mechanism of collided ribosome clearance by the ribosome quality control trigger complex.
Externí odkaz:
https://doaj.org/article/5436ac774d974902b0037e430ff18f6a
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-12 (2023)
The colliding ribosomes are ubiquitinated by the sensor protein Hel2, leading to noncanonical subunit dissociation by the ribosome associated quality control trigger (RQT) complex. Here the authors reveal the decoding mechanism of the ribosome ubiqui
Externí odkaz:
https://doaj.org/article/6cec7fce5f374befb8da3f894d3a96fd
Autor:
Ryosuke Ishimura, Afnan H. El-Gowily, Daisuke Noshiro, Satoko Komatsu-Hirota, Yasuko Ono, Mayumi Shindo, Tomohisa Hatta, Manabu Abe, Takefumi Uemura, Hyeon-Cheol Lee-Okada, Tarek M. Mohamed, Takehiko Yokomizo, Takashi Ueno, Kenji Sakimura, Tohru Natsume, Hiroyuki Sorimachi, Toshifumi Inada, Satoshi Waguri, Nobuo N. Noda, Masaaki Komatsu
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-16 (2022)
The UFM1 system, a ubiquitin-like conjugation system is crucial for endoplasmic reticulum (ER) homeostasis. Here, authors found that CYB5R3 is covalently conjugated with UFM1, which becomes a signal for ER-phagy, a selective autophagy of ER.
Externí odkaz:
https://doaj.org/article/fedd7858566a4c3695542e6f98801031
Autor:
Momoko Narita, Timo Denk, Yoshitaka Matsuo, Takato Sugiyama, Chisato Kikuguchi, Sota Ito, Nichika Sato, Toru Suzuki, Satoshi Hashimoto, Iva Machová, Petr Tesina, Roland Beckmann, Toshifumi Inada
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-16 (2022)
Collided ribosomes are marked by ubiquitination to induce quality control mechanisms. Here, authors show that mammalian disomes form a distinct structural interface, in which uS10 K63-linked polyubiquitination is critical for ribosome dissociation.
Externí odkaz:
https://doaj.org/article/fdef5afc077647cc8ee2226266299005
Autor:
Yoshitaka Matsuo, Toshifumi Inada
Publikováno v:
Biomolecules, Vol 13, Iss 2, p 317 (2023)
Genetic mutations, mRNA processing errors, and lack of availability of charged tRNAs sometimes slow down or completely stall translating ribosomes. Since an incomplete nascent chain derived from stalled ribosomes may function anomalously, such as by
Externí odkaz:
https://doaj.org/article/ffcbbddf380f43ea9c53ba4ac3bebe3d
Publikováno v:
STAR Protocols, Vol 2, Iss 3, Pp 100615- (2021)
Summary: Here, we describe a protocol for tRNA identification in the 60S ribosome-nascent peptide complex co-purified with Nuclear Export Mediator Factor (NEMF), a responsible factor for C-terminal alanine and threonine tailing of the nascent peptide
Externí odkaz:
https://doaj.org/article/e7ceb7b86cff4beba9b91b6e1cde93db
Autor:
Yuka Takehara, Hideki Yashiroda, Yoshitaka Matsuo, Xian Zhao, Akane Kamigaki, Tetsuo Matsuzaki, Hidetaka Kosako, Toshifumi Inada, Shigeo Murata
Publikováno v:
iScience, Vol 24, Iss 3, Pp 102145- (2021)
Summary: Ubiquitination is a major post-translational modification of ribosomal proteins. The role of ubiquitination in the regulation of ribosome functions is still being elucidated. However, the importance of ribosome deubiquitination remains uncle
Externí odkaz:
https://doaj.org/article/b429015d98e745319f25a5a4cdd66d49
Autor:
Yoshitaka Matsuo, Toshifumi Inada
Publikováno v:
Cell Reports, Vol 34, Iss 12, Pp 108877- (2021)
Summary: Ribosome collision because of translational stalling is recognized as a problematic event in translation by the E3 ubiquitin ligase Hel2, leading to non-canonical subunit dissociation followed by targeting of the faulty nascent peptides for
Externí odkaz:
https://doaj.org/article/12e36acbbfbf4fbb8ed823f4b352bfbe
Autor:
Peixun Han, Yuichi Shichino, Tilman Schneider-Poetsch, Mari Mito, Satoshi Hashimoto, Tsuyoshi Udagawa, Kenji Kohno, Minoru Yoshida, Yuichiro Mishima, Toshifumi Inada, Shintaro Iwasaki
Publikováno v:
Cell Reports, Vol 31, Iss 5, Pp - (2020)
Summary: Ribosome movement is not always smooth and is rather often impeded. For ribosome pauses, fundamental issues remain to be addressed, including where ribosomes pause on mRNAs, what kind of RNA/amino acid sequence causes this pause, and the phy
Externí odkaz:
https://doaj.org/article/931f5d3326cc4313b94bc11e5a4171bb