Výsledky vyhledávání - "Toshiaki Takezawa"

Akademický článek

Heat Shock-Induced Three-Dimensional-Like Proliferation of Normal Human Fibroblasts Mediated by Pressed Silk

Autor: Shigeki Inoue, Yoshio Kano, Yuji Wakatake, Chiyuki Takabayashi, Toshiaki Takezawa, Hirotoshi Motoda, Fukumi Hiragami

Publikováno v: International Journal of Molecular Sciences, Vol 10, Iss 11, Pp 4963-4976 (2009)

The aim of this study was to determine the optimal heat treatment conditions for enhancement of pressed silk-mediated 3D-like proliferation of normal human dermal fibroblasts, as well as to determine the responses to heat shock of cells and intracell

Externí odkaz: https://doaj.org/article/962cc43a1121417599b897fa6e390489

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Akademický článek

Cryopreservation in Situ of Cell Monolayers on Collagen Vitrigel Membrane Culture Substrata: Ready-to-Use Preparation of Primary Hepatocytes and ES Cells

Autor: Yoshitaka Miyamoto, Shin Enosawa, Tomoyo Takeuchi, Toshiaki Takezawa

Publikováno v: Cell Transplantation, Vol 18 (2009)

Cryopreservation is generally performed on cells in suspension. In the case of adherent cells such as hepatocytes, a loss of their ability to attach is a more serious problem than a decreased viability after cryopreservation. We herein report a novel

Externí odkaz: https://doaj.org/article/c675f864d82f4c82974b99e80c69bfa2

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Akademický článek

Collagen Vitrigel: A Novel Scaffold that can Facilitate a Three-Dimensional Culture for Reconstructing Organoids

Autor: Toshiaki Takezawa Ph.D., Katsuyuki Ozaki, Aya Nitani, Chiyuki Takabayashi, Tadashi Shimo-Oka

Publikováno v: Cell Transplantation, Vol 13 (2004)

Three-dimensional reconstructed organoids in vitro are valuable for not only regenerative medicine but also drug development. However, the manipulation of conventional three-dimensional cultures is not simple. We describe a nylon membrane ring-embedd

Externí odkaz: https://doaj.org/article/e729342e3e06424cba07afc145bd54c5

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Transplantation of Human Induced Pluripotent Stem Cell-Derived Airway Cells on Vitrigel Membrane into Rat Nasal Cavity

Autor: Yasuyuki Hayashi, Takeshi Tada, Toshiaki Takezawa, Masayoshi Kobayashi, Hiroe Ohnishi, Hideaki Okuyama, Fumihiko Kuwata, Koichi Omori, Masayoshi Yoshimatsu, Norio Yamamoto, Yuji Kitada, Masaru Yamashita

Publikováno v: Tissue Engineering Part A. 28:586-594

The nasal mucosa functions as a frontline biological defense against various foreign substances and pathogens. Maintaining homeostasis of the nasal epithelium is necessary to promote good health. Nasal epithelia are constantly replaced under normal c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e1e47b96f63e3f3bb4fc2000b534df44
https://doi.org/10.1089/ten.tea.2021.0071

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Collagen Vitrigel Membrane-Coated Nylon Line Prevents Stenosis After Conization of the Cervix Uteri

Autor: Takashi Akutagawa, Shuji Toda, Yasuhisa Sakata, Fumikazu Matsuhisa, Mizuki Tayama-Abe, Maki Futamata, Kei Nagase, Mariko Hashiguchi, Toshiaki Takezawa, Shigehisa Aoki, Masatoshi Yokoyama, Shuji Kitajima, Kotaro Yamaji, Sayuri Morito

Publikováno v: Tissue Engineering Part A. 27:1480-1489

Cervical stenosis is a postoperative complication of conization for uterine cervical malignancy, but a standard method of preventing this complication has yet to be established. Collagen vitrigel is a collagen-based biomaterial that has antifibrotic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a507ae1afc9017d6dbc98febb6c38d10
https://doi.org/10.1089/ten.tea.2020.0374

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Bi-layered carboxymethyl cellulose-collagen vitrigel dual-surface adhesion-prevention membrane

Autor: Yue Wang, Kei Kanie, Toshiaki Takezawa, Miki Horikawa, Kyoshiro Kaneko, Ayako Sugimoto, Aika Yamawaki-Ogata, Yuji Narita, Ryuji Kato

Publikováno v: Carbohydrate polymers. 285

During wound regeneration, both cell adhesion and adhesion-inhibitory functions must be controlled in parallel. We developed a membrane with dual surfaces by merging the properties of carboxymethyl cellulose (CMC) and collagen using vitrification. A

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67f4177b976cee4ebc46b8b16fc2cbde
https://pubmed.ncbi.nlm.nih.gov/35287853

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Multi-laboratory Validation Study of the Vitrigel-Eye Irritancy Test Method as an Alternative to In Vivo Eye Irritation Testing

Autor: Hajime Kojima, Takayuki Fukuda, Toshiaki Takezawa, Wannhsin Chen, Lim Chae-Hyung, Mika Watanabe, Hiroyuki Yamaguchi, Kunihiko Yamashita, Nicole Kleinstreuer, Takashi Sozu

Publikováno v: SC40202003260017
NARO成果DBa
C40202001310001_7962

Collagen vitrigel membranes (CVMs) comprising high-density collagen fibrils equivalent to in vivo connective tissues have been widely used in cell culture applications. A human corneal epithelium (hCE) model was previously developed by the Takezawa g

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c8935319718e446fa25020fbd30395a
https://doi.org/10.1177/0261192919886665

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Fabrication of a Corneal Model Composed of Corneal Epithelial and Endothelial Cells via a Collagen Vitrigel Membrane Functioned as an Acellular Stroma and Its Application to the Corneal Permeability Test of Chemicals

Autor: Hiroyuki Yamaguchi, Toshiaki Takezawa

Publikováno v: SC30201902210009
NARO成果DBa
OA
CC BY

A collagen vitrigel membrane (CVM) we developed can function as both a scaffold for cells and a pathway for chemicals. To extrapolate the corneal permeability of chemicals in vivo, we proposed six corneal models using the CVM. Thin and thick CVMs wer

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::211c2bb9a19495ffbf0251fe6a32a2ed
https://doi.org/10.1124/dmd.118.080820

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