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pro vyhledávání: '"Toshiaki Hiratsuka"'
Autor:
Toshiaki Hiratsuka
Publikováno v:
European Journal of Biochemistry. 270:3479-3485
Fluorescent and colored trinitrophenylated (TNP) analogs of ATP and GTP can interact with nucleotide-requiring enzymes and proteins as a substitute for the parent nucleotide. These analogs have strong binding affinities for most nucleotide-requiring
Autor:
Tsuyoshi Katoh, Toshiaki Hiratsuka
Publikováno v:
Journal of Biological Chemistry. 278:31891-31894
The esterification reagent 9-anthroylnitrile (ANN) reacts with a serine residue in the NH2-terminal 23-kDa peptide segment of myosin subfragment-1 heavy chain to yield a fluorescent S1 derivative labeled by the anthroyl group (Hiratsuka, T. (1989) J.
Autor:
Toshiaki Hiratsuka
Publikováno v:
Journal of Biological Chemistry. 274:29156-29163
To obtain a consistent view of the nucleotide-induced conformational changes around Cys697 (SH2) and Cys707 (SH1) in skeletal myosin subfragment-1 (S-1), the two thiols were labeled with the same environmentally sensitive fluorophore, 6-acyl-2-dimeth
Autor:
Toshiaki Hiratsuka
Publikováno v:
Biochemistry. 37:7167-7176
The noncovalent fluorescent probe 6-propionyl-2-(dimethylamino)naphthalene (prodan) binds stoichiometrically to myosin subfragment-1 (S-1) without affecting the ATPase and actin-binding properties of S-1. Neither ATP nor actin interferes with the pro
Autor:
Toshiaki Hiratsuka
Publikováno v:
Biophysical Journal. 72:843-849
A pyrene-labeled ATP (Pyr-ATP) in which a pyrene fluorophore is linked to the ribose moiety of ATP with a butyryl chain has been synthesized, together with the corresponding analog of ADP. The spectroscopic properties of two fluorescent analogs were
Autor:
Toshiaki Hiratsuka
Publikováno v:
Seibutsu Butsuri. 35:89-94
Myosin is one of the key protein components of biological force generating systems. The globular head region of the myosin molecule, subfragment-1 (S-1), contains the site for ATP binding and hydrolysis and the site for actin binding. Although the op
Autor:
Toshiaki Hiratsuka
Publikováno v:
Journal of Biological Chemistry. 268:24742-24750
4-Fluoro-7-sulfamoylbenzofurazan (ABDF) has a rather small fluorophore that is linked directly to the sulfur atom of thiols without a flexible alkyl chain (Imai, K., and Toyo'oka, T. (1987) Methods Enzymol. 143, 67-75). In the present study I examine
Publikováno v:
Neuroscience Research Supplements. 12:S135-S144
Autor:
Toshiaki Hiratsuka
Publikováno v:
Biochemistry. 45(4)
The fluorescent probe 3-[4-(3-phenyl-2-pyrazolin-1-yl)benzene-1-sulfonyl amido]phenylboronic acid (PPBA) acts as a fluorescent inhibitor for the ATPases of skeletal [Hiratsuka (1994) J. Biol. Chem. 269, 27251-27257] and Dictyostelium discoideum [Bobk
Autor:
Toshiaki, Hiratsuka
Publikováno v:
European journal of biochemistry. 270(17)
Fluorescent and colored trinitrophenylated (TNP) analogs of ATP and GTP can interact with nucleotide-requiring enzymes and proteins as a substitute for the parent nucleotide. These analogs have strong binding affinities for most nucleotide-requiring