High-level production of animal-free recombinant transferrin from saccharomyces cerevisiae

Autor: Goldenberg Hans, Evans Robert W, Tooth David J, Saxton Malcolm J, Morton Philip, Wilkinson Diane, Dodsworth Neil, Hay Joanna, Payne Tom, Finnis Christopher JA, Scheiber-Mojdehkar Barbara, Ternes Nina, Sleep Darrell

Publikováno v: Microbial Cell Factories, Vol 9, Iss 1, p 87 (2010)

Abstract Background Animal-free recombinant proteins provide a safe and effective alternative to tissue or serum-derived products for both therapeutic and biomanufacturing applications. While recombinant insulin and albumin already exist to replace t

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Method for the Purification of Endogenous Unanchored Polyubiquitin Chains.

Autor: Scott D; School of Life Sciences, Queen's Medical Centre, University of Nottingham, Nottingham, NG7 2UH, UK., Strachan J; Institute of Cell Biology, University of Edinburgh, Edinburgh, UK., Krishna VG; School of Life Sciences, Queen's Medical Centre, University of Nottingham, Nottingham, NG7 2UH, UK., Shaw B; School of Life Sciences, Queen's Medical Centre, University of Nottingham, Nottingham, NG7 2UH, UK., Tooth DJ; School of Life Sciences, Queen's Medical Centre, University of Nottingham, Nottingham, NG7 2UH, UK., Searle MS; School of Chemistry, University of Nottingham, University Park, Nottingham, NG7 2RD, UK.; Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK., Oldham NJ; School of Chemistry, University of Nottingham, University Park, Nottingham, NG7 2RD, UK., Layfield R; School of Life Sciences, Queen's Medical Centre, University of Nottingham, Nottingham, NG7 2UH, UK. robert.layfield@nottingham.ac.uk.

Publikováno v: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2016; Vol. 1449, pp. 203-13.