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Autor:
Katsutoshi Suetomi, Zhijian Lu, Tonia Heck, Javier Navarro, Karen J. Dunn, Thomas G. Wood, Deborah Prusak
Publikováno v:
Journal of Biological Chemistry. 274:11768-11772
We have probed an epitope sequence (His18-Pro19-Lys20-Phe21) in interleukin-8 (IL-8) by site-directed mutagenesis. This work shows that single and double Ala substitutions of His18 and Phe21 in IL-8 reduced up to 77-fold the binding affinity to IL-8