Zobrazeno 1 - 10
of 30
pro vyhledávání: '"Tomoya Kitatani"'
Autor:
Tsuyoshi Inoue, Ryota Murai, Yusuke Mori, Gen Sazaki, Tomoya Kitatani, Hiroshi Masuhara, Seiichiro Nakabayashi, Hiroaki Adachi, Hiroyoshi Matsumura, Yoichiroh Hosokawa, Hiroshi Yoshikawa, Satoshi Murakami, Kazufumi Takano
Publikováno v:
Crystal Growth & Design. 12:4334-4339
We developed a spatially precise, soft microseeding method for the production of single protein crystals that are suitable for X-ray crystallographic studies. We used focused femtosecond laser pulses to produce, via multiphoton absorption processes,
Autor:
Kazufumi Takano, Yoshinori Takahashi, Mika Hirose, Hiroyoshi Matsumura, Shigeru Sugiyama, Yusuke Mori, Tsuyoshi Inoue, Hiroaki Adachi, Satoshi Murakami, Tomoya Kitatani, Noriko Shimizu, Hitoshi Hasenaka
Publikováno v:
Journal of Crystal Growth. 312:73-78
Manual manipulation of protein crystals is often required in order to obtain X-ray diffraction (XRD) data, but the success of the manual operation depends on the experience and fortuity of the operators. Here, we demonstrated the processing of protei
Autor:
Yusuke Mori, Tomoya Kitatani, Hiroaki Adachi, Tsuyoshi Inoue, Hiroyoshi Matsumura, Ryota Murai, Yoshinori Takahashi, Hiroshi Yoshikawa, Gen Sazaki, Shigeru Sugiyama, Kazufumi Takano, Satoshi Murakami
Publikováno v:
Journal of Crystal Growth. 311(No. 3):956-959
We have performed femtosecond laser-induced nucleation of protein in agarose gel to study the crystallization dynamics. The use of agarose gel facilitates the monitoring of laser-induced crystallization from the focal point, because the gel forms a h
Autor:
Tooru Kimura, Taro Tamada, Sho Maki, Koushi Hidaka, Ryota Kuroki, Yusuke Mori, Hiroshi Yoshikawa, Yoshio Hayashi, Hiroaki Adachi, Shigeru Sugiyama, Hiroyoshi Matsumura, Yoshiaki Kiso, Satoshi Murakami, Tomoya Kitatani, Motoyasu Adachi, Shino Okada, Megumi Yamakami, Tsuyoshi Inoue, Kazufumi Takano
Publikováno v:
Acta Crystallographica Section F Structural Biology and Crystallization Communications. 64:1003-1006
This paper reports the crystallization and preliminary neutron diffraction measurements of HIV-1 protease, a potential target for anti-HIV therapy, complexed with an inhibitor (KNI-272). The aim of this neutron diffraction study is to obtain structur
Autor:
Shigeru Sugiyama, Syou Maki, Hiroaki Adachi, Yusuke Mori, Hisato Kawahara, Satoshi Murakami, Kazufumi Takano, Ryota Murai, Hiroshi Yoshikawa, Hiroyoshi Matsumura, Tsuyoshi Inoue, Takatomo Sasaki, Tomoya Kitatani
Publikováno v:
Journal of Crystal Growth. 310:2168-2172
We investigated the relationship between the flow produced by a rotary shaker and protein crystallization. Lysozyme was crystallized in a solution stirred by a rotary shaker at 50 rpm. The number of crystals grown in the stirring environment was less
Autor:
Syou Maki, Shigeru Sugiyama, Tsutomu Sasaki, Tsuyoshi Inoue, Hiroyoshi Matsumura, Hisato Kawahara, A. Kobayashi, Hitoshi Hasenaka, Tomoya Kitatani, Kazufumi Takano, Ryota Murai, Yusuke Mori, S. Okada, Hiroaki Adachi, S. Nakata, Hiroshi Yoshikawa, Satoshi Murakami
Publikováno v:
Journal of Synchrotron Radiation
To investigate quantitatively the effects of stirring on protein crystallization, a new stirring system which can agitate a protein solution, ∼100 nl, by providing Hagen–Poiseuille flow has been successfully developed.
To investigate quantit
To investigate quantit
Autor:
Hiroyoshi Matsumura, Tomoya Kitatani, Takatomo Sasaki, Hiroaki Adachi, Ryota Murai, Yusuke Mori, Syou Maki, Gen Sazaki, Satoshi Murakami, Shigeru Sugiyama, Kazufumi Takano, Hiroshi Yoshikawa, Tsuyoshi Inoue
Publikováno v:
Applied Physics a-Materials Science & Processing. 93(No. 4):911-915
We have investigated femtosecond laser-induced nucleation of hen egg-white lysozyme (HEWL) as a function of the laser pulse energy and pulse time width. This is the first recorded study to confirm that the femtosecond laser-induced nucleation of HEWL
Autor:
Tomoya Kitatani, Shigeru Shigeoka, Yoshihiro Nakamura, Kei Wada, Toshiji Tada, Takayoshi Kinoshita, Masahiro Tamoi
Publikováno v:
Acta Crystallographica Section F Structural Biology and Crystallization Communications. 62:727-730
The crystal structure of NADP-dependent apo-glyceraldehyde-3-phosphate dehydrogenase (apo-GAPDH) from Synechococcus PCC 7942 is reported. The crystal structure was solved by molecular replacement and refined to an R of 21.7% and R(free) of 27.5% at 2
Autor:
Shigeru Sugiyama, Ryota Murai, Tomoya Kitatani, Hiroyoshi Matsumura, Satoshi Murakami, Kazufumi Takano, Hiroaki Adachi, Tsuyoshi Inoue, Yoshinori Takahashi, Yusuke Mori
Publikováno v:
Japanese Journal of Applied Physics. 47:8995-8997
We previously developed Crystal Catcher CT-100, a tool for manipulating protein crystals that enables the direct capture of a crystal from solution using an adhesive. Here, we demonstrate the effectiveness of this tool in cryocrystallography with ins
Autor:
Kumsun Paku, Hiroyoshi Matsumura, Yusuke Mori, Tomoya Kitatani, Koji Tomoo, Tsuyoshi Inoue, Hiroaki Adachi, Satoshi Murakami, Shigeru Sugiyama, Masashi Mima, Syou Maki, Takahiro Iwamoto, Satomi Kita, Toshimasa Ishida, Hiroshi Yoshikawa, Chika Kawai, Kazufumi Takano
Publikováno v:
Acta Crystallogr Sect F Struct Biol Cryst Commun. 64(No. Pt 12):1125-7
The plasma-membrane Na(+)/Ca(2+) exchanger (NCX) regulates intracellular Ca(2+) levels in cardiac myocytes. Two Ca(2+)-binding domains (CBD1 and CBD2) exist in the large cytosolic loop of NCX. The binding of Ca(2+) to CBD1 results in conformational c