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pro vyhledávání: '"Tomohito Shimegi"'
Autor:
Satoko Noda, Sadaharu Ui, Tomohito Shimegi, Takashi Ohtsuki, Yuhsuke Takusagawa, Masami Kusunoki, Genji Kurisu
Publikováno v:
Protein & Peptide Letters. 18:825-830
The development of a stable L-BDH chimera was attempted by exchanging whole domains between two native structural analogs, L-BDH and meso-BDH, because the S-configuration specificity of L-BDH is valuable from the standpoint of its application but its
Autor:
Kaito Mochizuki, Sadaharu Ui, Takuji Oyama, Tomohito Shimegi, Masami Kusunoki, Takashi Ohtsuki
Publikováno v:
Protein and peptide letters. 22(3)
A chimeric (2S, 3S)-butanediol dehydrogenase (cLBDH) was engineered to have the strict (S)-configuration specificity of the (2S, 3S)-BDH (BsLBDH) derived from Brevibacterium saccharolyticum as well as the enzymatic stability of the (2R, 3S)-BDH (KpMB