Zobrazeno 1 - 10
of 288
pro vyhledávání: '"Tomohisa Hasunuma"'
Autor:
Shanga Chu, Yuji Haraguchi, Toru Asahi, Yuichi Kato, Akihiko Kondo, Tomohisa Hasunuma, Tatsuya Shimizu
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-10 (2024)
Abstract Large-scale production of cultured meat requires bulk culture medium containing growth-promoting proteins from animal serum. However, animal serum for mammalian cell culture is associated with high costs, ethical concerns, and contamination
Externí odkaz:
https://doaj.org/article/fa938b36270f45c197c7109277d5a8fb
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-9 (2024)
Abstract Cellobiose has received increasing attention in various industrial sectors, ranging from food and feed to cosmetics. The development of large-scale cellobiose applications requires a cost-effective production technology as currently used met
Externí odkaz:
https://doaj.org/article/10b3d00e060b47929825b92ac04d6647
Autor:
Musashi Takenaka, Kouhei Kamasaka, Kim Daryong, Keiko Tsuchikane, Seiha Miyazawa, Saeko Fujihana, Yoshimi Hori, Christopher J. Vavricka, Akira Hosoyama, Hiroko Kawasaki, Tomokazu Shirai, Michihiro Araki, Akira Nakagawa, Hiromichi Minami, Akihiko Kondo, Tomohisa Hasunuma
Publikováno v:
Microbial Cell Factories, Vol 23, Iss 1, Pp 1-12 (2024)
Abstract Background Computational mining of useful enzymes and biosynthesis pathways is a powerful strategy for metabolic engineering. Through systematic exploration of all conceivable combinations of enzyme reactions, including both known compounds
Externí odkaz:
https://doaj.org/article/eec63415caf84260b34d1edf12f160e1
Autor:
Yuichi Kato, Ryota Hidese, Mami Matsuda, Ryudo Ohbayashi, Hiroki Ashida, Akihiko Kondo, Tomohisa Hasunuma
Publikováno v:
Communications Biology, Vol 7, Iss 1, Pp 1-9 (2024)
Abstract Glycogen serves as a metabolic sink in cyanobacteria. Glycogen deficiency causes the extracellular release of distinctive metabolites such as pyruvate and 2-oxoglutarate upon nitrogen depletion; however, the mechanism has not been fully eluc
Externí odkaz:
https://doaj.org/article/014f918e275f452594e9c39949cd3c15
Autor:
Ryota Hidese, Ryudo Ohbayashi, Yuichi Kato, Mami Matsuda, Kan Tanaka, Sousuke Imamura, Hiroki Ashida, Akihiko Kondo, Tomohisa Hasunuma
Publikováno v:
Communications Biology, Vol 6, Iss 1, Pp 1-12 (2023)
Abstract The cyanobacterium Synechococcus elongatus PCC 7942 accumulates alarmone guanosine tetraphosphate (ppGpp) under stress conditions, such as darkness. A previous study observed that artificial ppGpp accumulation under photosynthetic conditions
Externí odkaz:
https://doaj.org/article/b952a86b94da4bc0864e8993c55dcc74
Autor:
Mikiko Uemura, Noriaki Maeshige, Atomu Yamaguchi, Xiaoqi Ma, Mami Matsuda, Yuya Nishimura, Tomohisa Hasunuma, Taketo Inoue, Jiawei Yan, Ji Wang, Hiroyo Kondo, Hidemi Fujino
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-10 (2023)
Abstract Macrophages play an important role as effector cells in innate immune system. Meanwhile, macrophages activated in a pro-inflammatory direction alter intracellular metabolism and damage intact tissues by increasing reactive oxygen species (RO
Externí odkaz:
https://doaj.org/article/e31eee905c444821ac20df8492565d92
l-Lactate treatment by photosynthetic cyanobacteria expressing heterogeneous l-lactate dehydrogenase
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-8 (2023)
Abstract l-Lactate is a major waste compound in cultured animal cells. To develop a sustainable animal cell culture system, we aimed to study the consumption of l-lactate using a photosynthetic microorganism. As genes involved in l-lactate utilizatio
Externí odkaz:
https://doaj.org/article/696e2a04ba8d4e68b2ab2ec9594ec598
Autor:
Hirotaka Kudo, Sho Ono, Kenta Abe, Mami Matsuda, Tomohisa Hasunuma, Tomoyasu Nishizawa, Munehiko Asayama, Hirofumi Nishihara, Shigeru Chohnan
Publikováno v:
Microbial Cell Factories, Vol 22, Iss 1, Pp 1-13 (2023)
Abstract Background Coenzyme A (CoA) is a carrier of acyl groups. This cofactor is synthesized from pantothenic acid in five steps. The phosphorylation of pantothenate is catalyzed by pantothenate kinase (CoaA), which is a key step in the CoA biosynt
Externí odkaz:
https://doaj.org/article/5375cfa8eb5c4f3d86d31981d33055bc
Autor:
Tomoki Oyama, Yuichi Kato, Ryota Hidese, Mami Matsuda, Minenosuke Matsutani, Satoru Watanabe, Akihiko Kondo, Tomohisa Hasunuma
Publikováno v:
Biotechnology for Biofuels and Bioproducts, Vol 15, Iss 1, Pp 1-14 (2022)
Abstract Background Microalgal lipid production has attracted global attention in next-generation biofuel research. Nitrogen starvation, which drastically suppresses cell growth, is a common and strong trigger for lipid accumulation in microalgae. We
Externí odkaz:
https://doaj.org/article/c55227422c3e481bb4aea150b7f89c43
Autor:
Yoichiro Ito, Misa Ishigami, Noriko Hashiba, Yasuyuki Nakamura, Goro Terai, Tomohisa Hasunuma, Jun Ishii, Akihiko Kondo
Publikováno v:
Microbial Biotechnology, Vol 15, Iss 9, Pp 2364-2378 (2022)
Summary In our previous study, we serendipitously discovered that protein secretion in the methylotrophic yeast Pichia pastoris is enhanced by a mutation (V50A) in the mating factor alpha (MFα) prepro‐leader signal derived from Saccharomyces cerev
Externí odkaz:
https://doaj.org/article/9ab13543e8bf4a56b68f62305629a389