Zobrazeno 1 - 10
of 53
pro vyhledávání: '"Tomohiko Akiyama"'
Autor:
Keisuke Noguchi, Hidefumi Suzuki, Ryota Abe, Keiko Horiuchi, Rena Onoguchi-Mizutani, Nobuyoshi Akimitsu, Shintaro Ogawa, Tomohiko Akiyama, Yoko Ike, Yoko Ino, Yayoi Kimura, Akihide Ryo, Hiroshi Doi, Fumiaki Tanaka, Yutaka Suzuki, Atsushi Toyoda, Yuki Yamaguchi, Hidehisa Takahashi
Publikováno v:
Cell Reports, Vol 43, Iss 9, Pp 114734- (2024)
Summary: Membrane-less subcellular compartments play important roles in various cellular functions. Although techniques exist to identify components of cellular bodies, a comprehensive method for analyzing both static and dynamic states has not been
Externí odkaz:
https://doaj.org/article/ff790441bf2945d7928f5e867e56e1c7
Autor:
Chie Kobori, Ryo Takagi, Ryo Yokomizo, Sakie Yoshihara, Mai Mori, Hiroto Takahashi, Palaksha Kanive Javaregowda, Tomohiko Akiyama, Minoru S. H. Ko, Kazuo Kishi, Akihiro Umezawa
Publikováno v:
Stem Cell Research & Therapy, Vol 14, Iss 1, Pp 1-10 (2023)
Abstract Background Melanocytes are an essential part of the epidermis, and their regeneration has received much attention because propagation of human adult melanocytes in vitro is too slow for clinical use. Differentiation from human pluripotent st
Externí odkaz:
https://doaj.org/article/fc63b996e0084d08a3e7fc3cc031bf5d
Autor:
Yuki Horisawa-Takada, Chisato Kodera, Kazumasa Takemoto, Akihiko Sakashita, Kenichi Horisawa, Ryo Maeda, Ryuki Shimada, Shingo Usuki, Sayoko Fujimura, Naoki Tani, Kumi Matsuura, Tomohiko Akiyama, Atsushi Suzuki, Hitoshi Niwa, Makoto Tachibana, Takashi Ohba, Hidetaka Katabuchi, Satoshi H. Namekawa, Kimi Araki, Kei-Ichiro Ishiguro
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-17 (2021)
The authors add to our knowledge of the transcriptional regulation of the meiotic program in mice spermatocytes, showing ZFP541 regulates meiotic prophase and transition to the division phase by being the target for upstream factors MEIOSIN/STRA8.
Externí odkaz:
https://doaj.org/article/ae1c01342b7a40449dbc60cd3eaf9267
Autor:
Sho Tanosaki, Tomohiko Akiyama, Sayaka Kanaami, Jun Fujita, Minoru S.H. Ko, Keiichi Fukuda, Shugo Tohyama
Publikováno v:
STAR Protocols, Vol 3, Iss 2, Pp 101360- (2022)
Summary: Here we describe a protocol to obtain highly pure cardiomyocytes and neurons from human induced pluripotent stem cells (hiPSCs) via metabolic selection processes. Compared to conventional purification protocols, this approach is easier to pe
Externí odkaz:
https://doaj.org/article/f83f4663619c40cd975bf6e5d53aa444
Autor:
Tomohiko Akiyama, Saeko Sato, Shigeru B. H. Ko, Osamu Sano, Sho Sato, Masayo Saito, Hiroaki Nagai, Minoru S. H. Ko, Hidehisa Iwata
Publikováno v:
Stem Cells Translational Medicine, Vol 10, Iss 4, Pp 572-581 (2021)
Abstract Gaucher disease, the most prevalent metabolic storage disorder, is caused by mutations in the glucocerebrosidase gene GBA1, which lead to the accumulation of glucosylceramide (GlcCer) in affected cells. Gaucher disease type 1 (GD1), although
Externí odkaz:
https://doaj.org/article/78f55af7e0164003bcf214e45756fb37
Autor:
Koji Nishihara, Takahiro Shiga, Eri Nakamura, Tomohiko Akiyama, Takashi Sasaki, Sadafumi Suzuki, Minoru S.H. Ko, Norihiro Tada, Hideyuki Okano, Wado Akamatsu
Publikováno v:
Stem Cell Reports, Vol 12, Iss 2, Pp 305-318 (2019)
Summary: Although pluripotent stem cells can generate various types of differentiated cells, it is unclear why lineage-committed stem/progenitor cells derived from pluripotent stem cells are decelerated and why the differentiation-resistant propensit
Externí odkaz:
https://doaj.org/article/40748e62b3d34c28a682ce70c8b24703
Autor:
Sho Tanosaki, Shugo Tohyama, Jun Fujita, Shota Someya, Takako Hishiki, Tomomi Matsuura, Hiroki Nakanishi, Takayo Ohto-Nakanishi, Tomohiko Akiyama, Yuika Morita, Yoshikazu Kishino, Marina Okada, Hidenori Tani, Yusuke Soma, Kazuaki Nakajima, Hideaki Kanazawa, Masahiro Sugimoto, Minoru S.H. Ko, Makoto Suematsu, Keiichi Fukuda
Publikováno v:
iScience, Vol 23, Iss 9, Pp 101535- (2020)
Summary: The role of lipid metabolism in human pluripotent stem cells (hPSCs) is poorly understood. We have used large-scale targeted proteomics to demonstrate that undifferentiated hPSCs express different fatty acid (FA) biosynthesis-related enzymes
Externí odkaz:
https://doaj.org/article/97fb6446db2549fc8be5d95c5a409f8f
Autor:
Yuhki Nakatake, Shigeru B.H. Ko, Alexei A. Sharov, Shunichi Wakabayashi, Miyako Murakami, Miki Sakota, Nana Chikazawa, Chiaki Ookura, Saeko Sato, Noriko Ito, Madoka Ishikawa-Hirayama, Siu Shan Mak, Lars Martin Jakt, Tomoo Ueno, Ken Hiratsuka, Misako Matsushita, Sravan Kumar Goparaju, Tomohiko Akiyama, Kei-ichiro Ishiguro, Mayumi Oda, Norio Gouda, Akihiro Umezawa, Hidenori Akutsu, Kunihiro Nishimura, Ryo Matoba, Osamu Ohara, Minoru S.H. Ko
Publikováno v:
Cell Reports, Vol 31, Iss 7, Pp - (2020)
Summary: Transcription factors (TFs) play a pivotal role in determining cell states, yet our understanding of the causative relationship between TFs and cell states is limited. Here, we systematically examine the state changes of human pluripotent em
Externí odkaz:
https://doaj.org/article/5e01196c36404cffa69b4492e2cf07ae
Autor:
Hideomi Ida, Tomohiko Akiyama, Keiichiro Ishiguro, Sravan K. Goparaju, Yuhki Nakatake, Nana Chikazawa-Nohtomi, Saeko Sato, Hiromi Kimura, Yukihiro Yokoyama, Masato Nagino, Minoru S. H. Ko, Shigeru B. H. Ko
Publikováno v:
Stem Cell Research & Therapy, Vol 9, Iss 1, Pp 1-10 (2018)
Abstract Background Transplantation of pancreatic β cells generated in vitro from pluripotent stem cells (hPSCs) such as embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) has been proposed as an alternative therapy for diabetes.
Externí odkaz:
https://doaj.org/article/2d82226ca15a41c395522aebc3a4c712
Autor:
Tomohiko Akiyama, Saeko Sato, Nana Chikazawa-Nohtomi, Atsumi Soma, Hiromi Kimura, Shunichi Wakabayashi, Shigeru B. H. Ko, Minoru S. H. Ko
Publikováno v:
Scientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
Abstract Direct generation of skeletal muscle cells from human pluripotent stem cells (hPSCs) would be beneficial for drug testing, drug discovery, and disease modelling in vitro. Here we show a rapid and robust method to induce myogenic differentiat
Externí odkaz:
https://doaj.org/article/5d62cce54ee541739436beee7b392604