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of 3
pro vyhledávání: '"Tommi Timoharju"'
Autor:
Juha Rouvinen, Sasikala Anbarasan, Tommi Timoharju, Matti Leisola, Ossi Turunen, Janice Barthomeuf, Ossi Pastinen
Publikováno v:
Journal of Molecular Catalysis B: Enzymatic. 118:62-69
Sulfolobus acidocaldarius β-glycosidase (BGAL_SULAC) was used as an extremophilic enzyme model to study the effect of mutations close to the catalytic residues on the enzyme activity and the pH-activity profile. We report here the results for three
Autor:
Johanna Kallio, Nina Hakulinen, Tommi Timoharju, Mirko M. Maksimainen, Ossi Turunen, Juha Rouvinen
Publikováno v:
Acta Crystallographica Section F Structural Biology and Crystallization Communications. 65:767-769
An extracellular beta-galactosidase from Trichoderma reesei was crystallized from sodium cacodylate buffer using polyethylene glycol (PEG) as a precipant. Crystals grown by homogenous streak-seeding belonged to space group P1, with unit-cell paramete
Autor:
Mirko M. Maksimainen, Johanna Kallio, Tommi Timoharju, Juha Rouvinen, Ossi Turunen, Nina Hakulinen
Publikováno v:
Journal of structural biology. 174(1)
We have determined the crystal structure of Trichoderma reesei (Hypocrea jecorina) β-galactosidase (Tr-β-gal) at a 1.2A resolution and its complex structures with galactose, IPTG and PETG at 1.5, 1.75 and 1.4A resolutions, respectively. Tr-β-gal i