Structural basis of rapid actin dynamics in the evolutionarily divergent Leishmania parasite

Autor: Tommi Kotila, Hugo Wioland, Muniyandi Selvaraj, Konstantin Kogan, Lina Antenucci, Antoine Jégou, Juha T. Huiskonen, Guillaume Romet-Lemonne, Pekka Lappalainen

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-18 (2022)

The authors report here the structure-function analysis of highly divergent actin from Leishmania parasite. The study reveals remarkably rapid dynamics of parasite actin as well as the underlying molecular basis, thus providing insight into evolution

Externí odkaz: https://doaj.org/article/b51e8c37b37c487a80e957a5cb198fc3

Mechanism of Borrelia immune evasion by FhbA-related proteins.

Autor: Konstantin Kogan, Karita Haapasalo, Tommi Kotila, Robin Moore, Pekka Lappalainen, Adrian Goldman, Taru Meri

Publikováno v: PLoS Pathogens, Vol 18, Iss 3, p e1010338 (2022)

Immune evasion facilitates survival of Borrelia, leading to infections like relapsing fever and Lyme disease. Important mechanism for complement evasion is acquisition of the main host complement inhibitor, factor H (FH). By determining the 2.2 Å cr

Externí odkaz: https://doaj.org/article/e2881d03e0494f5d88d863c96523e8bc

Structural basis of actin monomer re-charging by cyclase-associated protein

Autor: Tommi Kotila, Konstantin Kogan, Giray Enkavi, Siyang Guo, Ilpo Vattulainen, Bruce L. Goode, Pekka Lappalainen

Publikováno v: Nature Communications, Vol 9, Iss 1, Pp 1-12 (2018)

Depolymerized ADP-actin monomers must be recharged with ATP for new rounds of filament assembly. Here the authors show that cyclase-associated protein (CAP) catalyzes actin nucleotide exchange in vivo and their CAP–actin complex structure reveals t

Externí odkaz: https://doaj.org/article/0d7ef003bbce4a12bf5c7b11adc2fba6

Unusual actin-binding mechanism and the role of profilin in actin dynamics of trypanosomatid parasites

Autor: Andrea Vizcaíno-Castillo, Tommi Kotila, Konstantin Kogan, Ryuji Yanase, Juna Como, Lina Antenucci, Alphee Michelot, Jack D. Sunter, Pekka Lappalainen

Diseases caused byLeishmania, andTrypanosomaparasites, such as leishmaniasis and African sleeping sickness, are a major health problem in tropical countries. Due to their complex life cycle involving both vertebrate and insect hosts, and > 1 billion

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b00e2c0e81ad5f2a78c253309653a4b4
https://doi.org/10.1101/2023.01.06.522972

A functional family of fluorescent nucleotide analogues to investigate actin dynamics and energetics

Autor: Jenna Elliott, Jessica Colombo, Pekka Lappalainen, Konstantin Kogan, Alphée Michelot, Tommi Kotila, Audrey Guillotin, Adrien Antkowiak

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
Nature Communications
Nature Communications, Nature Publishing Group, 2021, 12 (1), ⟨10.1038/s41467-020-20827-4⟩
Nature Communications, Nature Publishing Group, 2021, 12, pp.548. ⟨10.1038/s41467-020-20827-4⟩
Nature Communications, 2021, 12 (1), ⟨10.1038/s41467-020-20827-4⟩
Nature Communications, 2021, 12, pp.548. ⟨10.1038/s41467-020-20827-4⟩

Actin polymerization provides force for vital processes of the eukaryotic cell, but our understanding of actin dynamics and energetics remains limited due to the lack of high-quality probes. Most current probes affect dynamics of actin or its interac

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6dc536806899363eef32858a0c0e224
https://doaj.org/article/887eda43f8084479b43243044a31eee3