Zobrazeno 1 - 10
of 43
pro vyhledávání: '"Tomislav Maricic"'
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-8 (2022)
Some DNA sequences are refractory to CRISPR-Cas9 cleavage, partially due to gRNA misfolding. Here the authors engineer gRNAs to prevent misfolding and further enhanced their stability by chemical modifications allowing robust genome editing regardles
Externí odkaz:
https://doaj.org/article/3c3aabf79278436ca06ea285e96b069d
Autor:
Lukas Bokelmann, Olaf Nickel, Tomislav Maricic, Svante Pääbo, Matthias Meyer, Stephan Borte, Stephan Riesenberg
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-8 (2021)
Current SARS-CoV-2 diagnostic methods are sensitive yet poorly suited to testing whole communities on a regular basis. Here the authors present Cap-iLAMP that tests gargle lavage samples with an improved colorimetric RT-LAMP.
Externí odkaz:
https://doaj.org/article/232860e3bc4b45058d30368f379f2efe
Autor:
Tomás Suchý, Isabell Kaczmarek, Tomislav Maricic, Christian Zieschang, Torsten Schöneberg, Doreen Thor, Ines Liebscher
Publikováno v:
Adipocyte, Vol 10, Iss 1, Pp 631-645 (2021)
Cell lines recapitulating physiological processes can represent alternatives to animal or human studies. The 3T3-L1 cell line is used to mimic adipocyte function and differentiation. Since transfection of 3T3-L1 cells is difficult, we used a modified
Externí odkaz:
https://doaj.org/article/55f47f5971ea489b9fdb752081c1730e
Autor:
Vita Stepanova, Kaja Ewa Moczulska, Guido N Vacano, Ilia Kurochkin, Xiangchun Ju, Stephan Riesenberg, Dominik Macak, Tomislav Maricic, Linda Dombrowski, Maria Schörnig, Konstantinos Anastassiadis, Oliver Baker, Ronald Naumann, Ekaterina Khrameeva, Anna Vanushkina, Elena Stekolshchikova, Alina Egorova, Anna Tkachev, Randall Mazzarino, Nathan Duval, Dmitri Zubkov, Patrick Giavalisco, Terry G Wilkinson, David Patterson, Philipp Khaitovich, Svante Pääbo
Publikováno v:
eLife, Vol 10 (2021)
We analyze the metabolomes of humans, chimpanzees, and macaques in muscle, kidney and three different regions of the brain. Although several compounds in amino acid metabolism occur at either higher or lower concentrations in humans than in the other
Externí odkaz:
https://doaj.org/article/fd1a3282c2934c4483b85c46f214b2b5
Autor:
Tomislav Maricic, Olaf Nickel, Ayinuer Aximu-Petri, Elena Essel, Marie Gansauge, Philipp Kanis, Dominik Macak, Julia Richter, Stephan Riesenberg, Lukas Bokelmann, Hugo Zeberg, Matthias Meyer, Stephan Borte, Svante Pääbo
Publikováno v:
PLoS ONE, Vol 15, Iss 12, p e0244824 (2020)
SARS-CoV-2 causes substantial morbidity and mortality in elderly and immunocompromised individuals, particularly in retirement homes, where transmission from asymptomatic staff and visitors may introduce the infection. Here we present a cheap and fas
Externí odkaz:
https://doaj.org/article/8a1c1150aec14acf837e3851ebb86c05
Autor:
Stephan Riesenberg, Tomislav Maricic
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
Small molecule inhibitors can influence the choice of repair pathways, enhancing nucleotide substitution and gene integration in CRISPR-mediated genome editing. Here the authors introduce CRISPY, a mix of small molecules that can enhance precise edit
Externí odkaz:
https://doaj.org/article/f237a600df754fcf880c21624126704e
Autor:
Felipe Mora-Bermúdez, Farhath Badsha, Sabina Kanton, J Gray Camp, Benjamin Vernot, Kathrin Köhler, Birger Voigt, Keisuke Okita, Tomislav Maricic, Zhisong He, Robert Lachmann, Svante Pääbo, Barbara Treutlein, Wieland B Huttner
Publikováno v:
eLife, Vol 5 (2016)
Human neocortex expansion likely contributed to the remarkable cognitive abilities of humans. This expansion is thought to primarily reflect differences in proliferation versus differentiation of neural progenitors during cortical development. Here,
Externí odkaz:
https://doaj.org/article/7176d3b52d8e4e29b0c7c168e0dae321
Autor:
Tomislav Maricic, Svante Pääbo
Publikováno v:
BioTechniques, Vol 46, Iss 1, Pp 51-57 (2009)
To increase the yield of DNA sequence generated by the 454 technology from small amounts of starting DNA, we investigated the efficiency of each step in the 454 library preparation process. We find that the last step, when the single-stranded library
Externí odkaz:
https://doaj.org/article/d16a8267852e498381e306cb4bfffbc9
Autor:
Hernán A Burbano, Richard E Green, Tomislav Maricic, Carles Lalueza-Fox, Marco de la Rasilla, Antonio Rosas, Janet Kelso, Katherine S Pollard, Michael Lachmann, Svante Pääbo
Publikováno v:
PLoS ONE, Vol 7, Iss 3, p e32877 (2012)
Several previous comparisons of the human genome with other primate and vertebrate genomes identified genomic regions that are highly conserved in vertebrate evolution but fast-evolving on the human lineage. These human accelerated regions (HARs) may
Externí odkaz:
https://doaj.org/article/9dad5171832240a8a5ab6a82beb1d0e8
Publikováno v:
PLoS ONE, Vol 5, Iss 11, p e14004 (2010)
BACKGROUND: To utilize the power of high-throughput sequencers, target enrichment methods have been developed. The majority of these require reagents and equipment that are only available from commercial vendors and are not suitable for the targets t
Externí odkaz:
https://doaj.org/article/ee2d43ed63d44b1d89518882da98892a