Výsledky vyhledávání - "Tomasz Krusiński"

Equilibrium Analysis of the DNA Binding Domain of the Ultraspiracle Protein Interaction with the Response Element from the hsp27 Gene Promoter—the Application of Molecular Beacon Technology

Autor: Iwona Grad, Tomasz Krusiński, Piotr Dobryszycki, Marta Wietrzych, Andrzej Ożyhar

Publikováno v: Journal of Fluorescence. 18:1-10

Ecdysteroids initiate molting and metamorphosis in insects via a receptor which belongs to the superfamily of nuclear receptors. The ecdysone receptor consists of two proteins: the ecdysone receptor (EcR) and the ultraspiracle (Usp). The EcR-Usp dime

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc0f318cac7d82db7a1152e566303f6e
https://doi.org/10.1007/s10895-007-0285-y

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The DNA-Binding Domain of the Ultraspiracle Drives Deformation of the Response Element Whereas the DNA-Binding Domain of the Ecdysone Receptor Is Responsible for a Slight Additional Change of the Preformed Structure

Autor: Marek Orłowski, Dorota Sawicka, Piotr Michaluk, Tomasz Krusiński, Grzegorz Rymarczyk, Michał Jakób, Marian Kochman, Piotr Dobryszycki, Iwona Grad, Andrzej Ożyhar, Agnieszka Kowalska

Publikováno v: Biochemistry. 45:668-675

Ecdysteroids control molting and metamorphosis in insects via a heterodimeric complex of two nuclear receptors, the ecdysone receptor (EcR) and ultraspiracle protein (Usp). We used fluorescence resonance energy transfer (FRET) to study the topology o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a953fd97e6d644485a9deeda5961b140
https://doi.org/10.1021/bi051354b

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The application of an immobilized molecular beacon for the analysis of the DNA binding domains from the ecdysteroid receptor proteins Usp and EcR's interaction with the hsp27 response element

Autor: Tomasz Krusiński, Andrzej Ożyhar, Piotr Dobryszycki, Anna Laskowska

Publikováno v: Journal of biomolecular screening. 13(9)

The nonstandard molecular beacon described in this article consists of 2 fragments, each built of a short single-stranded oligonucleotide sequence and a double-stranded sequence. One of these hybridization probes, labeled with a fluorescence donor (f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52b44284622fbf21439067db40f040c9
https://pubmed.ncbi.nlm.nih.gov/18812572

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Dual FRET assay for detecting receptor protein interaction with DNA

Autor: Andrzej Ożyhar, Tomasz Krusiński, Piotr Dobryszycki

Publikováno v: Nucleic Acids Research

We present here a new assay that is based on the idea of the molecular beacon. This assay makes it possible to investigate two proteins interacting with DNA at two binding sites that are close to each other. The effectiveness of the test depends on t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5b0969acb49fc375725fc33ea1cb148
https://doi.org/10.1093/nar/gkq049

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