Zobrazeno 1 - 10
of 87
pro vyhledávání: '"Tomasz Heyduk"'
Autor:
Lydia C. Koehler, Zachary R. Grese, Alliny C. S. Bastos, Lohany D. Mamede, Tomasz Heyduk, Yuna M. Ayala
Publikováno v:
Frontiers in Neuroscience, Vol 16 (2022)
Loss of TDP-43 protein homeostasis and dysfunction, in particular TDP-43 aggregation, are tied to amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). TDP-43 is an RNA binding protein tightly controlling its own expression levels th
Externí odkaz:
https://doaj.org/article/19cb82a47dad4510a086a80a181564fb
Autor:
Carl Frieden, Robert Flaumenhaft, Tomasz Heyduk, Nicola Pozzi, Mathivanan Chinnaraj, David A. Barrios
Publikováno v:
Chembiochem
Folding of newly synthesized proteins in the endoplasmic reticulum is assisted by several families of enzymes. One such family is the protein disulfide isomerases (PDIs). PDIs are oxidoreductases, capable of forming new disulfide bonds or breaking ex
Autor:
Ewa Heyduk, Tomasz Heyduk
Publikováno v:
Nucleic Acids Research
Promoter escape involves breaking of the favourable contacts between RNA polymerase (RNAP) and the promoter to allow transition to an elongation complex. The sequence of DNA template that is transcribed during promoter escape (ITS; Initially Transcri
Detection of specific antibodies has numerous research, therapeutic and diagnostic applications. Short peptide ligands that bind specifically to antibodies with continuous epitopes can be derived from epitope mapping experiments. Short peptide ligand
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01648f470b96b44a4e6a657fcf76e000
https://europepmc.org/articles/PMC6185743/
https://europepmc.org/articles/PMC6185743/
Autor:
Jesse Hauver, Robert Landick, Andrey Feklistov, Karl-Heinz Altmann, Brian Bae, Agnieszka Lass-Napiorkowska, Tomasz Heyduk, Markus Kalesse, Seth A. Darst, Florian Glaus
Trapping RNA polymerase in the act The enzyme RNA polymerase (RNAP) finds promoter elements in the genome, separates (or “melts”) the DNA strands, and transcribes the template DNA strand to give RNA. A mobile clamp in RNAP plays a key role in ini
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3bfbf24a2beab4a068844a56151e3b3
https://europepmc.org/articles/PMC5696265/
https://europepmc.org/articles/PMC5696265/
Autor:
Tomasz Heyduk, Je Ko
Publikováno v:
Biochemical Journal. 463:135-144
Promoter escape by RNA polymerase, the transition between the initiation and elongation, is a critical step that defines transcription output at many promoters. In the present study we used a real-time fluorescence assay for promoter melting and esca
RNA polymerase (RNAP) backtracking is a backward sliding of the enzyme along DNA and RNA. It plays important roles in many essential processes in bacteria and in eukaryotes. We describe here a fluorescence-based approach that allows a real-time obser
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c53b7b83af3c88921300a7727655747
https://europepmc.org/articles/PMC5921838/
https://europepmc.org/articles/PMC5921838/
Publikováno v:
Biophysical Chemistry. 159:73-81
Bacterial RNA polymerase (RNAP) interacts with conserved − 10 and − 35 promoter elements to recognize the promoter and to form an open complex in which DNA duplex around transcription start site melts. Using model DNA constructs (fork junction DN
Publikováno v:
Diabetes
OBJECTIVE Glucose-stimulated islet insulin or C-peptide secretion experiments are a fundamental tool for studying and assessing islet function. The goal of this work was to develop Ab-based fluorescent homogenous sensors that would allow rapid, inexp
Autor:
Tomasz Heyduk, Ling Tian
Publikováno v:
Analytical Chemistry. 81:5218-5225
The homogeneous immunosensor design described here utilizes the bivalent nature of the antibody. Antigen peptide is conjugated using flexible linkers with short complementary oligonucleotides (signaling oligonucleotides), each of which containing a f