Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Tomasz H. Kowalczyk"'
Autor:
David M. Yurek, Anita M. Fletcher, Matthew McShane, Tomasz H. Kowalczyk, Linas Padegimas, Marcy R. Weatherspoon, Michael D. Kaytor, Mark J. Cooper, Assem G. Ziady
Publikováno v:
Molecular Imaging, Vol 10 (2011)
In this study, we used bioluminescence imaging (BLI) to track long-term transgene activity following the transfection of brain cells using a nonviral gene therapy technique. Formulations of deoxyribonucleic acid (DNA) combined with 30-mer lysine poly
Externí odkaz:
https://doaj.org/article/74fd1eaad961438ba622f377fd517651
Autor:
David M. Yurek Ph.D., Anita M. Flectcher, Tomasz H. Kowalczyk, Linas Padegimas, Mark J. Cooper
Publikováno v:
Cell Transplantation, Vol 18 (2009)
Previously it was established that infusion of glial cell line-derived neurotrophic factor (GDNF) protein into grafts of embryonic dopamine cells has a neurotrophic effect on the grafted cells. In this study we used a nonviral technique to transfer t
Externí odkaz:
https://doaj.org/article/4e8e06173631474999fb88f01cec4ca2
Autor:
Assem G. Ziady, Kim B. Seroogy, Tomasz H. Kowalczyk, Anita M. Fletcher, Joseph Molter, Linas Padegimas, David M. Yurek, Mark J. Cooper, George M. Smith
Publikováno v:
Molecular Therapy. 17(4):641-650
This study demonstrates proof of concept for delivery and expression of compacted plasmid DNA in the central nervous system. Plasmid DNA was compacted with polyethylene glycol substituted lysine 30-mer peptides, forming rod-like nanoparticles with di
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cadac0c75cd08acc6ed026e83806c23e
Autor:
Robert C. Moen, Christopher R. Gedeon, Osman Muhammad, Jennifer M. Payne, Pamela B. Davis, Assem G. Ziady, Will Quan, Angela Peischl, Tamara L. Fink, Virginia Stillwell, Susannah L. Hyatt, Sharon M. Oette, Tomasz H. Kowalczyk, J. E. Seng, Mark J. Cooper
Publikováno v:
Molecular Therapy. 8:948-956
Nanoparticles containing DNA compacted with poly-l-lysine modified on an N-terminal cysteine with polyethylene glycol can effectively transfect cells of the airway epithelium when applied by the luminal route. To evaluate the toxicity of these nanopa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e6ae1e8c42068e303b4995495818ceaf
https://doi.org/10.1016/j.ymthe.2003.09.002
https://doi.org/10.1016/j.ymthe.2003.09.002
Publikováno v:
Analytical Biochemistry. 212:194-205
A method has been developed for determining endopolyphosphatase (polyphosphate depolymerase, EC 3.6.1.10) activity. The enzyme catalyzes the hydrolysis of inorganic polyphosphates [poly(Ps)] by cleaving internal phosphoanhydride bonds without removal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b77c447f14452eea46e5625c05767e94
https://doi.org/10.1006/abio.1993.1312
https://doi.org/10.1006/abio.1993.1312
Publikováno v:
Cell Transplantation, Vol 18 (2009)
Previously it was established that infusion of glial cell line-derived neurotrophic factor (GDNF) protein into grafts of embryonic dopamine cells has a neurotrophic effect on the grafted cells. In this study we used a nonviral technique to transfer t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a481ac26fe4e6f27f83b4ebfadea66fb
https://doaj.org/article/4e8e06173631474999fb88f01cec4ca2
https://doaj.org/article/4e8e06173631474999fb88f01cec4ca2
Autor:
Nancy J. Meilander, Tomasz H. Kowalczyk, Murali K Pasumarthy, Ravi V. Bellamkonda, Mark J. Cooper
Publikováno v:
Journal of controlled release : official journal of the Controlled Release Society. 88(2)
Non-viral gene therapy typically results in low transfection efficiencies and transient gene expression. To address these limitations, two sustained delivery systems capable of releasing functional, compacted DNA for over 50 days were designed. A luc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a798258e74d401e90f5a8786bad8ab89
https://pubmed.ncbi.nlm.nih.gov/12628338
https://pubmed.ncbi.nlm.nih.gov/12628338
Publikováno v:
Biochemistry. 35(30)
Polyphosphate glucokinase from Mycobacterium tuberculosis catalyzes the phosphorylation of glucose using inorganic polyphosphates [poly(P)] or ATP. The steady-state kinetic mechanisms of the poly(P)- and ATP-dependent glucokinase reactions were inves
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3cad15f5ebd84f144a59c7d7bf870db3
https://pubmed.ncbi.nlm.nih.gov/8703950
https://pubmed.ncbi.nlm.nih.gov/8703950
Publikováno v:
Biochemistry. 35(21)
Polyphosphate glucokinase [EC 2.7.1.63] catalyzes the phosphorylation of glucose using either inorganic polyphosphate [poly(P)] or ATP as the phosphoryl donor. Both activities purified from Propionibacterium shermanii are the functional properties of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a71cdfa885c38e005ad02f6fb6c7638
https://pubmed.ncbi.nlm.nih.gov/8639629
https://pubmed.ncbi.nlm.nih.gov/8639629
Autor:
Michael W. Konstan, Pamela B. Davis, Jeffrey S. Wagener, Kathleen A. Hilliard, Robert C. Stern, Laura J.H. Milgram, Tomasz H. Kowalczyk, Susannah L. Hyatt, Tamara L. Fink, Christopher R. Gedeon, Sharon M. Oette, Jennifer M. Payne, Osman Muhammad, Assem G. Ziady, Robert C. Moen, Mark J. Cooper
Publikováno v:
Human Gene Therapy; Dec2004, Vol. 15 Issue 12, p1255-1269, 15p